• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional analysis of novel transporter involved in intra-cellular transport of nicotine biosynthetic intermediates.

Research Project

Project/Area Number 23780109
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Research InstitutionKobe Pharmaceutical University

Principal Investigator

SHITAN Nobukazu  神戸薬科大学, 薬学部, 講師 (20547880)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords植物 / アルカロイド / トランスポーター / ニコチン / 転流 / 物質生産 / タバコ / 二次代謝
Research Abstract

Plants produce a large number of secondary metabolites. Some metabolites are often highly accumulated in particular organs of medicinal plants. The movement of biosynthetic intermediates within the organelles in a cell is also suggested. However, little is known how metabolites are transported across membranes and how these movements are involved in metabolite production. Tobacco T408 is a plastid-localized transporter which might regulate intermediate transport in a cell. We altered expression level of T408 by producing stable transformants of tobacco plants and cultured cells. Transgenic cultured cells produced higher level of alkaloids than control cells under jasmonate treatment. These data suggested that productions of secondary metabolites are increased by regulating the expression level of specific transporter in a cell.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (12 results)

All 2013 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (9 results) Remarks (1 results)

  • [Journal Article] Functional Analysis of Jasmonic Acid-Responsive Secondary Metabolite Transporters2013

    • Author(s)
      士反 伸和
    • Journal Title

      Methods in Molecular Biology

      Volume: 印刷中

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional Analysis of JasmonicAcid-Responsive Secondary Metabolite

    • Author(s)
      Shitan N., Sugiyama A., Yazaki K.
    • Journal Title

      Transporters Methods in Molecular Biology

      Volume: (印刷中)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Alain Goossens、DirkInze、守安正恭、矢崎一史タバコNCS1型トランスポーターNt-T408のクローニングと機能解析2013

    • Author(s)
      士反伸和、南翔太、宮坂萌菜、林田南帆
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] アルカロイド輸送能の改変植物を用いた環境適応機構の解明と物質生産2013

    • Author(s)
      士反伸和、矢崎一史、杉山暁史
    • Organizer
      第223回生存圏シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2013-03-13
    • Related Report
      2012 Final Research Report
  • [Presentation] タバコNCS1 型トランスポーターNt-T408 のクローニングと機能解析2013

    • Author(s)
      士反 伸和
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] アルカロイド輸送能の改変植物を用いた環境適応機構の解明と物質生産2013

    • Author(s)
      士反 伸和
    • Organizer
      第223回生存圏シンポジウム
    • Place of Presentation
      京都大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] タバコ新規トランスポーターNt-T408のクローニング及び発現解析2012

    • Author(s)
      塩見裕子、南翔太、横田美咲、士反伸和、江口咲百合、Alain Goossens、Dirk Inze、矢崎一史、守安正恭
    • Organizer
      第7回トランスポーター研究会
    • Place of Presentation
      京都
    • Year and Date
      2012-06-09
    • Related Report
      2012 Final Research Report
  • [Presentation] タバコ新規トランスポーターNt-T408のクローニング及び発現解析2012

    • Author(s)
      塩見 裕子 (士反 伸和)
    • Organizer
      第7回トランスポーター研究会
    • Place of Presentation
      京都大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 「植物が医薬品原料を生産する仕組み」~タバコにおけるニコチン転流機構の解明~2011

    • Author(s)
      士反 伸和
    • Organizer
      第26回静岡大学GRLバイオサイエンスセミナー(招待講演)
    • Place of Presentation
      静岡
    • Related Report
      2011 Research-status Report
  • [Presentation] 植物の生体防御を担うアルカロイド輸送2011

    • Author(s)
      士反 伸和
    • Organizer
      第84回日本生化学会大会シンポジウム(招待講演)
    • Place of Presentation
      京都
    • Related Report
      2011 Research-status Report
  • [Presentation] ニコチン生産と転流に関わるタバコ新規トランスポーターの解析2011

    • Author(s)
      士反 伸和 他
    • Organizer
      第6回トランスポーター研究会
    • Place of Presentation
      仙台
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.kobepharma-u.ac.jp/rsch/rsch_04b.html

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi