Structure-activity relationship of paralytic factors of green lacewings

• Project/Area Number: 23780118
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Ehime University
• Principal Investigator: NISHIWAKI Hisahi 愛媛大学, 農学部, 助教 (30508784)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 生物活性物質 / ニッポンクサカゲロウ / 麻痺活性物質 / 農薬科学 / クサカゲロウ / 昆虫制御物質

Research Abstract

A fraction containing a paralytic substance purified from Chrysoperla nipponensisusing the various column chromatographies was found to show the acute toxicity against houseflies. A target band of the SDS PAGE gel was digested by proteases to elucidate parts of its amino acid sequence using the LC-MS/MS. Some PCR products were then amplified using the degenerated primers, which were designed based on the elucidated amino acid sequences.

Research Products

• [Journal Article] Synthesis of imidacloprid derivatives with a chiral alkylated imidazolidine ring and evaluation of their insecticidal activity and affinity to the nicotinic acetylcholine receptor. (2012)
  • Author(s): Nishiwaki, H., Kuriyama, M., Nagaoka, H., Kato, A., Yamauchi, S., Shuto, Y.
  • Journal Title: Bioorg. Med. Chem. Volume: 20 (21) Pages: 6305-6312
  • Peer Reviewed
• [Journal Article] Synthesis of imidacloprid derivatives with a chiral alkylated imidazolidine ring and evaluation of their insecticidal activity and affinity to the nicotinic acetylcholine receptor (2012)
  • Author(s): Nishiwaki, H.; Kuriyama, M.; Nagaoka, H.; Kato, A.; Akamatsu, M.; Yamauchi, S.; Shuto, Y.
  • Journal Title: Bioorganic and Medicinal Chemistry Volume: 20 Issue: 21 Pages: 3605-6312
  • DOI: 10.1016/j.bmc.2012.09.007
  • Peer Reviewed
• [Presentation] ニッポンクサカゲロウの殺虫活性成分の単離 (2012)
  • Author(s): 松浦茉佑・西脇寿・原有助・久保卓也・山内聡・首藤義博
  • Organizer: 第27回農薬デザイン研究会
  • Place of Presentation: 東京
  • Year and Date: 2012-11-08
• [Presentation] クサカゲロウ由来の麻痺活性物質 (2012)
  • Author(s): 松浦茉佑・西脇寿・原有助・菅原卓也・山内聡・首藤義博
  • Organizer: 日本農芸化学会中四国支部第33回講演会
  • Place of Presentation: 松山
  • Year and Date: 2012-06-02
• [Presentation] 天然由来の昆虫制御物質 (2012)
  • Author(s): 西脇寿
  • Organizer: 日本農芸化学会中四国支部 第18回市民フォーラム
  • Place of Presentation: 松山
  • Year and Date: 2012-05-26
• [Presentation] 天然由来の昆虫制御物質
  • Author(s): 西脇寿
  • Organizer: 日本農芸化学会中四国支部 第１８回市民フォーラム
  • Place of Presentation: 松山
  • Invited
• [Presentation] クサカゲロウ由来の麻痺活性物質
  • Author(s): 松浦茉佑，西脇寿，原有助，菅原卓也，山内聡，首藤義博
  • Organizer: 日本農芸化学会中四国支部第３３回講演会
  • Place of Presentation: 松山