Enzymatic synthesis of polyphenol-glycosides with stimulatory activity on Treg differentiation

• Project/Area Number: 23780129
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Shinshu University
• Principal Investigator: KATAYAMA Shigeru 信州大学, 農学部, 助教 (30443922)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 食品機能 / 免疫寛容 / ポリフェノール / 配糖体 / アレルギー

Research Abstract

We synthesized newly polyphenol glycosides via a rutinase-catalyzed transglycosylation reaction. We then prepared a newly in vitro assay system using DCs differentiated from human monocytic THP-1 for the screening of anti-allergic compounds. Significant decrease in HLA-DR expression was observed in the apple procyanidins and caffeic acid-rutinosides-treated cells in the stimulation of OVA. These suggest that the newly developed polyphenols glycosides should be contribute to anti-allergic agents with suppressive effect on antigen presentation.

Research Products

• [Journal Article] Apple polyphenols suppress antigen presentation of ovalbumin by THP-1-derived dendritic cells (2013)
  • Author(s): Katayama S, Kukita T, Ishikawa E, Nakashima S, Masuda S, Kanda T, Akiyama H, Teshima R, Nakamura S
  • Journal Title: Food Chem Volume: 138 Issue: 2-3 Pages: 757-761
  • DOI: 10.1016/j.foodchem.2012.10.076
  • Peer Reviewed
• [Presentation] THP-1由来樹状細胞を用いた食品成分のアレルゲン性および抗アレルギー作用 の評価 (2013)
  • Author(s): 片山茂
  • Organizer: 日本動物細胞工学会2013年度大会
  • Place of Presentation: ホテルフジタ福井（福井）
  • Invited
• [Presentation] Development of human dendritic cell-based in vitro assays for assessment of the potential allergenicity of foods and its anti-allergic compounds (2012)
  • Author(s): Katayama S.
  • Organizer: ISNFF2012
  • Place of Presentation: USA
  • Year and Date: 2012-12-05
• [Presentation] ルチン分解酵素ルチナーゼを用いた新規フェノール化合物配糖体ルチノシド合成の最適化 (2012)
  • Author(s): 山内雄貴、片山茂、加藤幸、真壁秀文、中村宗一郎
  • Organizer: 日本農芸化学会中部支部第165回例会
  • Place of Presentation: 愛知
  • Year and Date: 2012-10-27
• [Presentation] THP-1由来樹状細胞の確立とアレルゲン性評価への応用 (2012)
  • Author(s): 片山茂、久木田卓弥、穐山浩、中村宗一郎
  • Organizer: 日本食品化学会2012年度大会
  • Place of Presentation: 北海道
  • Year and Date: 2012-06-22
• [Presentation] リンゴ由来プロシアニジンの抗原提示抑制効果 (2012)
  • Author(s): 久木田卓弥、片山茂、中島翔平、舛田晋、神田智正、穐山浩、中村宗一郎
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] THP-1由来樹状細胞の確立とアレルゲン性評価への応用 (2012)
  • Author(s): 片山茂
  • Organizer: 日本食品化学会2012年度大会
  • Place of Presentation: 五島軒本店（北海道）
• [Presentation] Development of human dendritic cell-based in vitro assays for assessment of the potential allergenicity of foods and its anti-allergic compounds (2012)
  • Author(s): 片山茂
  • Organizer: ISNFF2012
  • Place of Presentation: Courtyard King Kamehameha's Kona Beach Hotel（USA）
• [Presentation] ルチン分解酵素ルチナーゼを用いた新規フェノール化合物配糖体ルチノシド合成の最適化 (2012)
  • Author(s): 山内雄貴
  • Organizer: 日本農芸化学会中部支部第165回例会
  • Place of Presentation: 名古屋大学（愛知）
• [Presentation] リンゴ由来プロシアニジンの抗原提示抑制効果 (2012)
  • Author(s): 久木田卓弥，片山茂，中島翔平，舛田晋，神田智正，穐山浩，中村宗一郎
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学
• [Presentation] 樹状細胞様THP-1細胞を用いた抗原提示能抑制物質の探索 (2011)
  • Author(s): 久木田卓弥、片山茂、石川えり、中島翔平、舛田晋、神田智正、穐山浩、中村宗一郎
  • Organizer: 第4回食品薬学シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2011-10-29
• [Presentation] 樹状細胞様THP-1細胞を用いた抗原提示能抑制物質の探索 (2011)
  • Author(s): 久木田卓弥，片山茂，石川えり，中島翔平，舛田晋，神田智正，穐山浩，中村宗一郎
  • Organizer: 第4回食品薬学シンポジウム
  • Place of Presentation: 慶応義塾大学薬学部