Degradation of b-glucan absorbed from intestine and the effect of degraded b-glucan on the intestinal immune function
| Project/Area Number |
23780131
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
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| Research Institution | Shizuoka University |
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Principal Investigator |
HINO Shingo 静岡大学, (連合)農学研究科(研究院), 助教 (70547025)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | β-グルカン / マクロファージ / 活性酸素 / 腸上皮細胞 / β－グルカン |
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| Research Abstract |
Using the extracellular domain of Dectin-1 as b-glucan-specific probes, we succeeded in detection of soluble and Dectin-1-reactive b-glucan discharged from macrophage cell lines as well as mouse peritoneal macrophages, which had phagocytized insoluble b-glucan particles. The RAW264.7 cell culture-supernatant containing the discharged b-glucan stimulated RAW264.7 cells, resulting in the induction of cytokine expression. Such discharge of Dectin-1-reactive b-glucan from macrophage cells was inhibited by either NADPH oxidase inhibitors or radical scavengers. These results suggest that degraded but Dectin-1-reactive b-glucan is discharged from macrophage cells phagocytizing insoluble b-glucan particles and stimulates the other phagocytes.
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] Commensal microbe-derived butyrate induces colonic regulatory T cells2013
Author(s)
Yukihiro Furusawa, Yuuki Obata, Shinji Fukuda, Takaho A. Endo, Gaku Nakato, Daisuke Takahashi, Yumiko Nakanishi, Chikako Uetake, Keiko Kato, Tamotsu Kato, Masumi Takahashi, Noriko N. Fukuda, Shinnosuke Murakami, Eiji Miyauchi, Shingo Hino, Koji Atarashi, Satoshi Onawa, Yumiko Fujimura, Trevor Lockett, Julie M. Clarke, David L. Topping, Masaru Tomita, Shohei Hori, Osamu Ohara, Tatsuya Morita, Haruhiko Koseki, J un Kikuchi, Kenya Honda, Koji Hase and Hiroshi Ohno
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Journal Title
Nature
Volume: 506
Issue: 7480
Pages: 446-450
DOI
Related Report
Peer Reviewed
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