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Degradation of b-glucan absorbed from intestine and the effect of degraded b-glucan on the intestinal immune function

Research Project

Project/Area Number 23780131
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Food science
Research InstitutionShizuoka University

Principal Investigator

HINO Shingo  静岡大学, (連合)農学研究科(研究院), 助教 (70547025)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsβ-グルカン / マクロファージ / 活性酸素 / 腸上皮細胞 / β-グルカン
Research Abstract

Using the extracellular domain of Dectin-1 as b-glucan-specific probes, we succeeded in detection of soluble and Dectin-1-reactive b-glucan discharged from macrophage cell lines as well as mouse peritoneal macrophages, which had phagocytized insoluble b-glucan particles. The RAW264.7 cell culture-supernatant containing the discharged b-glucan stimulated RAW264.7 cells, resulting in the induction of cytokine expression. Such discharge of Dectin-1-reactive b-glucan from macrophage cells was inhibited by either NADPH oxidase inhibitors or radical scavengers. These results suggest that degraded but Dectin-1-reactive b-glucan is discharged from macrophage cells phagocytizing insoluble b-glucan particles and stimulates the other phagocytes.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (37 results)

All 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (28 results) (of which Invited: 2 results) Remarks (1 results)

  • [Journal Article] Measurement of Barley β-Glucan Concentration in the Plasma by Sandwich ELISA Using Rat Dectin-12013

    • Author(s)
      Shingo Hino, Hikaru Sato, Tsukasa Matsuda, Tatsuya Morita
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 77 Issue: 2 Pages: 413-415

    • DOI

      10.1271/bbb.120788

    • NAID

      10031164754

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Non-Effect of an Antibiotic Treatment on Dietary Fiber-Induced Goblet Cell Proliferation in the Ileum of Rats2013

    • Author(s)
      Hino S, Saita H, Morita T
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 77 Issue: 8 Pages: 1782-1784

    • DOI

      10.1271/bbb.130241

    • NAID

      10031195835

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Slower Fermentation Rate of Potato Starch Relative to High-amylose Cornstarch Contributes to the Higher Proportion of Cecal Butyrate in Rats2013

    • Author(s)
      Morita T, Hino S, Ito A, Han KH , Shimada K, Fukushima M
    • Journal Title

      Bioscience of Microbiota, Food and Health

      Volume: 32 Issue: 4 Pages: 149-156

    • DOI

      10.12938/bmfh.32.149

    • NAID

      130004972270

    • ISSN
      2186-3342
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Commensal microbe-derived butyrate induces colonic regulatory T cells2013

    • Author(s)
      Yukihiro Furusawa, Yuuki Obata, Shinji Fukuda, Takaho A. Endo, Gaku Nakato, Daisuke Takahashi, Yumiko Nakanishi, Chikako Uetake, Keiko Kato, Tamotsu Kato, Masumi Takahashi, Noriko N. Fukuda, Shinnosuke Murakami, Eiji Miyauchi, Shingo Hino, Koji Atarashi, Satoshi Onawa, Yumiko Fujimura, Trevor Lockett, Julie M. Clarke, David L. Topping, Masaru Tomita, Shohei Hori, Osamu Ohara, Tatsuya Morita, Haruhiko Koseki, J un Kikuchi, Kenya Honda, Koji Hase and Hiroshi Ohno
    • Journal Title

      Nature

      Volume: 506 Issue: 7480 Pages: 446-450

    • DOI

      10.1038/nature12721

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Low-Methoxyl Pectin Stimulates Small Intestinal Mucin Secretion Irrespective of Goblet Cell Proliferation and Is Characterized by Jejunum Muc2 Upregulation in Rats.2013

    • Author(s)
      Shingo Hino, Kei Sonoyama, Hiroyuki Bito, Hirokazu Kawagishi, Seiichiro Aoe, Tatsuya Morita
    • Journal Title

      Journal of Nutrition

      Volume: 143(1) Issue: 1 Pages: 34-40

    • DOI

      10.3945/jn.112.167064

    • NAID

      120006732616

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Discharge of solubilized and Dectin-1-reactive β-glucan from macrophage cells phagocytizing insoluble β-glucan particles: involvement of reactive oxygen species (ROS)-driven degradation.2012

    • Author(s)
      Shingo Hino, Aki Kito, Remi Yokoshima, Ryosuke Sugino, Kenzi Oshima, Tatsuya Morita, Tetsuya Okajima, Daita Nadano, Koji Uchida, Tsukasa Matsuda
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 421(2) Issue: 2 Pages: 329-34

    • DOI

      10.1016/j.bbrc.2012.04.009

    • NAID

      120004770876

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Small intestinal goblet cell proliferation induced by ingestion of soluble and insoluble dietary fiber is characterized by an increase in sialylated mucins in rats.2012

    • Author(s)
      Shingo Hino, Naoki Takemura, Kei Sonoyama, Akio Morita, Hirokazu Kawagishi, Seiichiro Aoe, Tatsuya Morita
    • Journal Title

      Journal of Nutrition

      Volume: 142(8) Issue: 8 Pages: 1429-36

    • DOI

      10.3945/jn.112.159731

    • NAID

      120006729391

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ameliorating Effects of Short-Chain Inulin-Like Fructans on the Healing Stage of Trinitrobenzene Sulfonic Acid-Induced Colitis in Rats2011

    • Author(s)
      Shingo Hino, Hiroyuki Ito, Hiroyuki Bito, Hirokazu Kawagishi, Tatsuya Morita
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Issue: 11 Pages: 2169-2174

    • DOI

      10.1271/bbb.110460

    • NAID

      10030396728

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 難消化性デキストリン類のラット消化管内動態2013

    • Author(s)
      半田 圭, 濱口徳寿,平井宏和, 高橋良輔,尾藤寛之, 日野真吾, 森田達也
    • Organizer
      第67回日本栄養・食糧学会
    • Place of Presentation
      名古屋大学(愛知)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 小腸上皮組織による食物繊維の認識とムチン分泌促進作用2013

    • Author(s)
      日野真吾
    • Organizer
      第67回日本栄養・食糧学会
    • Place of Presentation
      名古屋大学(愛知)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] β-Glucanの免疫修飾作用と腸管吸収2012

    • Author(s)
      日野真吾
    • Organizer
      シーズ&ニーズビジネスマッチング研究発表会-高齢化社会・健康・食品産業-
    • Place of Presentation
      ホテルアソシア静岡
    • Related Report
      2013 Final Research Report
  • [Presentation] 消化管におけるβ-glucanの上皮細胞およびマクロファージによる認識2012

    • Author(s)
      日野真吾
    • Organizer
      第11回Hindgut Club Japanサテライトミーティング(第66回日本栄養・食糧学会大会)
    • Place of Presentation
      東北大学
    • Related Report
      2013 Final Research Report
  • [Presentation] 盲腸内IgAおよびムチン濃度に及ぼす フラクトオリゴ糖の効果は摂取期間によって劇変する2012

    • Author(s)
      福田智紀, 小村美香, 日野真吾, 園山慶, 森田達也
    • Organizer
      第17回日本食物繊維学会学術集会
    • Place of Presentation
      中村学園大学(福岡県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 成分栄養剤の摂取は小腸部位特異的にシアロムチン陽性杯細胞を増加させる2012

    • Author(s)
      日野真吾,伊藤文乃,森田達也:
    • Organizer
      第17回日本食物繊維学会学術集会
    • Place of Presentation
      中村学園大学(福岡県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 食物繊維摂取時の杯細胞数・ムチン増加作用への腸内細菌の関与2012

    • Author(s)
      日野真吾,最田裕善,森田達也
    • Organizer
      第66回日本栄養・食糧学会大会
    • Place of Presentation
      東北大学(宮城県)
    • Related Report
      2012 Research-status Report
  • [Presentation] ペクチンの化学情報は杯細胞からのムチン分泌を促進する2012

    • Author(s)
      福田智紀,尾藤寛之,日野真吾,園山慶,森田達也
    • Organizer
      第66回日本栄養・食糧学会大会
    • Place of Presentation
      東北大学(宮城県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 消化管におけるβ-glucanの上皮細胞およびマクロファージによる認識2011

    • Author(s)
      日野真吾
    • Organizer
      第17回Hindgut Club JAPANシンポジウム
    • Place of Presentation
      専修大学
    • Related Report
      2013 Final Research Report
  • [Presentation] β-グルカンの腸管からの吸収と分解機構2011

    • Author(s)
      日野真吾
    • Organizer
      日本農芸化学会中部支部第163回例会
    • Place of Presentation
      東海軒会館
    • Related Report
      2013 Final Research Report
  • [Presentation] β-Glucan and Its Implication for Health Benefits2011

    • Author(s)
      Hino S
    • Organizer
      where is it incorporated from?、2nd Korea-Japan Joint Symposium and Graduate Students Forum
    • Place of Presentation
      Kyungpook National University (Korea)
    • Related Report
      2013 Final Research Report
  • [Presentation] 成分栄養剤の摂取は十二指腸,空腸部でシアロムチン陽性杯細胞を増加させる

    • Author(s)
      日野真吾,伊藤文乃,森田達也
    • Organizer
      第18回Hind Gut Club Japan
    • Place of Presentation
      専修大学(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] β-Glucanの免疫修飾作用と腸管吸収

    • Author(s)
      日野真吾
    • Organizer
      シーズ&ニーズビジネスマッチング研究発表会-高齢化社会・健康・食品産業-(静岡大学食品・生物産業創出拠点第31回研究会)
    • Place of Presentation
      ホテルアソシア静岡(静岡県)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] βグルカンは腸吸収上皮細胞の経細胞輸送機能により吸収される

    • Author(s)
      日野真吾、横嶋麗実、鬼頭亜姫、灘野大太、森田達也、松田幹
    • Organizer
      第65回日本栄養・食糧学会
    • Place of Presentation
      お茶の水女子大学(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] キトサン摂取は杯細胞数の変動とは無関係に小腸ムチン分泌量を増加させる

    • Author(s)
      半田 圭、尾藤 寛之、伊藤 弘幸、日野 真吾、森田 達也
    • Organizer
      第65回日本栄養・食糧学会
    • Place of Presentation
      お茶の水女子大学(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] 高体積・高粘度食物繊維の摂取による小腸杯細胞応答は期間非依存的なシアロムチン分泌型の 増加による

    • Author(s)
      福田 智紀、河田 伊織、伊藤 弘幸、日野 真吾、森田 達也
    • Organizer
      第65回日本栄養・食糧学会
    • Place of Presentation
      お茶の水女子大学(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] 小麦グリアジンによる食物アレルギーのマウスモデル - IgE 応答の誘導と脱顆粒およびアナフィラキシーの誘発-

    • Author(s)
      平野 可奈、日野 真吾、灘野 大太、水野 幸子、松田 幹
    • Organizer
      第65回日本栄養・食糧学会
    • Place of Presentation
      お茶の水女子大学(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] 卵白タンパク質の腸管吸収とアナフィラキシー誘発性:Caco-2細胞とマウスを用いた比較解析

    • Author(s)
      秋山 友香、平野 可奈、日野 真吾、松原 毅、灘野 大太、松田 幹
    • Organizer
      第65回日本栄養・食糧学会
    • Place of Presentation
      お茶の水女子大学(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] β-Glucan and Its Implication for Health Benefits: where is it incorporated from?

    • Author(s)
      Shingo HINO
    • Organizer
      2nd Korea-Japan Joint Symposium and Graduate Students Forum "Convergence Technology of Bio and Nano Science"(招待講演)
    • Place of Presentation
      大韓民国慶北大学校(韓国)
    • Related Report
      2011 Research-status Report
  • [Presentation] 除菌ラットにおける食物繊維摂取時の杯細胞数増加作用 ;エンドトキシン関与の解析

    • Author(s)
      日野真吾,最田裕善,森田達也
    • Organizer
      日本食物繊維学会第16回学術集会
    • Place of Presentation
      独立行政法人国立健康・栄養研究所(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] ペクチンの化学情報は,杯細胞からのムチン分泌を促進すると同時に,腸間膜リンパ節のTh-1/Th-2バランスを修飾する

    • Author(s)
      尾藤寛之,日野真吾,園山慶,森田達也
    • Organizer
      日本食物繊維学会第16回学術集会
    • Place of Presentation
      独立行政法人国立健康・栄養研究所(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] 食物繊維による杯細胞増加作用は中心静脈栄養,経管(胃瘻)栄養時にも観察される

    • Author(s)
      伊藤文乃,土井彩友美,河田伊織,日野真吾,森田達也
    • Organizer
      日本食物繊維学会第16回学術集会
    • Place of Presentation
      独立行政法人国立健康・栄養研究所(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] β-Glucan and Its Implication for Health Benefits: where is it incorporated from?

    • Author(s)
      Shingo HINO
    • Organizer
      Shizuoka University International Symposium 2011-Initiatives for Crossing Boundaries within Science and Technology-(招待講演)
    • Place of Presentation
      B-nest (静岡)
    • Related Report
      2011 Research-status Report
  • [Presentation] β-グルカンの腸管からの吸収と分解機構

    • Author(s)
      日野 真吾
    • Organizer
      日本農芸化学会中部 支部第163回例会(招待講演)
    • Place of Presentation
      東海軒会館(静岡)
    • Related Report
      2011 Research-status Report
  • [Presentation] 消化管におけるβ-glucanの上皮細胞およびマクロファージによる認識

    • Author(s)
      日野 真吾
    • Organizer
      第17回  Hindgut Club Japanシンポジウム(招待講演)
    • Place of Presentation
      専修大学(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] イネ種子に含まれるイネ科植物花粉アレルゲンホモログタンパク質はアレルギー患者 IgEと反応する

    • Author(s)
      平野 可奈、日野 真吾、大島 健司、岡島 徹也、灘野 大太、宇理須 厚雄、松田 幹
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学(京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 食物繊維摂取時の杯細胞数・ムチン増加作用への腸内細菌の関与

    • Author(s)
      日野 真吾、最田 裕善、森田 達也
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学(京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 腸管とアレルギー

    • Author(s)
      日野 真吾
    • Organizer
      第13回静岡ライフサイエンスシンポジウム「明日、何を食べますか?-静岡発!!食の安全と健康-」(招待講演)
    • Place of Presentation
      静岡県立大学(静岡)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.agr.shizuoka.ac.jp/abc/morita_t/index.html

    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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