Protective role of flavonoids in the stress-induced intestinal barrier dysfunction

• Project/Area Number: 23780135
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Hiroshima University
• Principal Investigator: SUZUKI Takuya 広島大学, 大学院・生物圏科学研究科, 准教授 (30526695)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: フラボノイド / ストレス / 消化管バリア / 消化管バリア機能

Research Abstract

In this study, we investigated the ameliorative effects of flavonoids on the stress-induced intestinal barrier defect. Two flavonoids, quercetin and hesperetin, affect the expression and distribution of tight junction proteins, resulting in the increased integrity of intestinal barrier. These findings indicate the novel function of dietary flavonoids and could contribute to the intestinal health.

Research Products

• [Journal Article] Dietary polyphenols modulate intestinal barrier defects 3 and inflammation in a murine model of colitis (2013)
  • Author(s): Mizuki Shigeshiro
  • Journal Title: J Functional Foods Volume: 印刷中 Issue: 2 Pages: 949-955
  • DOI: 10.1016/j.jff.2013.02.008
  • Peer Reviewed
• [Journal Article] Supplemental Naringenin Prevents Intestinal Barrier Defects and Inflammation in Colitic Mice (2013)
  • Author(s): Tomoyo Azuma
  • Journal Title: J Nutr Volume: 印刷中 Issue: 6 Pages: 827-834
  • DOI: 10.3945/jn.113.174508
  • Peer Reviewed
• [Journal Article] Regulation of intestinal epithelial permeability by tight junctions. (2013)
  • Author(s): Takuya Suzuki
  • Journal Title: Cell Mol Life Scie Volume: 70 Issue: 4 Pages: 631-659
  • DOI: 10.1007/s00018-012-1070-x
  • Peer Reviewed
• [Journal Article] Regulation of Intestinal Barrier Function by Dietary Polyphenols (2013)
  • Author(s): Takuya Suzuki
  • Journal Title: Current Nutrition & Food Science Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Repeated exposure to water immersion stress reduces the Muc2 gene level in the rat colon via two distinct mechanisms (2012)
  • Author(s): Shigeshiro M, Tanabe S, and Suzuki T
  • Journal Title: Brain Behav Immun Volume: 26 Pages: 1061-1065
  • Peer Reviewed
• [Journal Article] Differential effects of flavonoids on barrier integrity in human intestinal Caco-2 cells (2012)
  • Author(s): Noda S, Tanabe S, and Suzuki T
  • Journal Title: J Agric Food Chem Volume: 60 Pages: 4628-4633
  • Peer Reviewed
• [Journal Article] Effect of D-alanine in teichoic acid from the Streptococcus thermophilus cell wall on the barrier-protection of intestinal epithelial cells (2012)
  • Author(s): Miyauchi E, Morita M, Rossi M, Morita H, Suzuki T, and Tanabe S
  • Journal Title: Biosci Biotechnol Biochem Volume: 76 Pages: 283-288
  • NAID: 10030399670
  • Peer Reviewed
• [Journal Article] Glycochenodeoxycholic acid promotes proliferation of intestinal epithelia via reduction of cyclic AMP and increase in H2AX phosphorylation after exposure to gamma-rays (2012)
  • Author(s): Ishizuka S, Shiwaku M, Hagio M, Suzuki T, Hira T, and Hara H
  • Journal Title: Biomed Res Volume: 33 Pages: 159-165
  • Peer Reviewed
• [Journal Article] Repeated exposure to water immersion stress reduces the Muc2 gene level in the rat colon via two distinct mechanisms. (2012)
  • Author(s): Mizuki Shigeshiro
  • Journal Title: Brain Behav Immun Volume: 26 Issue: 7 Pages: 1061-1065
  • DOI: 10.1016/j.bbi.2012.05.016
  • Peer Reviewed
• [Journal Article] Differential Effects of Flavonoids on Barrier Integrity in Human Intestinal Caco-2 Cells. (2012)
  • Author(s): Noda S, Tanabe S, Suzuki T.
  • Journal Title: J Agric Food Chem. Volume: 60 Issue: 18 Pages: 4628-4633
  • DOI: 10.1021/jf300382h
  • Peer Reviewed
• [Journal Article] Effect of D-Alanine in Teichoic Acid from the Streptococcus thermophilus Cell Wall on the Barrier-Protection of Intestinal Epithelial Cells (2012)
  • Author(s): Miyauchi E, Morita M, Rossi M, Morita H, Suzuki T, Tanabe S
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 2 Pages: 283-288
  • DOI: 10.1271/bbb.110646
  • NAID: 10030399670
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Kaempferol enhances intestinal barrier function through the cytoskeletal association and expression of tight junction proteins in Caco-2 cells (2011)
  • Author(s): Suzuki T, Tanabe S, and Hara H
  • Journal Title: J Nutr Volume: 141 Pages: 87-94
  • Peer Reviewed
• [Journal Article] Interleukin-6 (IL-6) regulates claudin-2 expression and tight junction permeability in intestinal epithelium (2011)
  • Author(s): Suzuki T, Yoshinaga N, and Tanabe S
  • Journal Title: J Biol Chem Volume: 286 Pages: 31263-31271
  • Peer Reviewed
• [Journal Article] Protein kinase Czeta phosphorylates occludin and promotes assembly of epithelial tight junctions (2011)
  • Author(s): Jain S, Suzuki T, Seth A, Samak G, and Rao RK.
  • Journal Title: Biochem J Volume: 437 Pages: 289-299
  • Peer Reviewed
• [Journal Article] Contrasting effects of ERK on tight junction integrity in differentiated and under-differentiated Caco-2 cell monolayers (2011)
  • Author(s): Aggarwal S, Suzuki T, Taylor WL, Bhargava A, and Rao RK
  • Journal Title: Biochem J Volume: 433 Pages: 51-63
  • Peer Reviewed
• [Journal Article] A partially degraded product of phytate suppresses the proliferation of HCT116 colorectal cancer cells (2011)
  • Author(s): Ishizuka S, Saito K, Suzuki T, Lee J, and Hara H
  • Journal Title: Food Chemistry Volume: 125 Pages: 1219-1225
  • NAID: 120002911948
  • Peer Reviewed
• [Journal Article] Streptococcus thermophilus ST28 ameliorates colitis in mice partially by suppression of inflammatory Th17 cells (2011)
  • Author(s): Ogita T, Nakashima M, Morita H, Saito Y, Suzuki T, and Tanabe S
  • Journal Title: J Biomed Biotechnol Pages: 378417-378417
  • Peer Reviewed
• [Journal Article] Suppression of Th17 response by Streptococcus thermophilus ST28 through induction of IFN-gamma (2011)
  • Author(s): Ogita T, Tanii Y, Morita H, Suzuki T, and Tanabe S
  • Journal Title: Int J Mol Med Volume: 28 Pages: 817-822
  • Peer Reviewed
• [Journal Article] Protein kinase Cζ phosphorylates occludin and promotes assembly of epithelial tight junctions (2011)
  • Author(s): Suneet JAIN, Takuya SUZUKI, Ankur SETH, Geetha SAMAK and Radhakrishna RAO
  • Journal Title: Biochem. J. Volume: 437 Issue: 2 Pages: 289-299
  • DOI: 10.1042/bj20110587
  • Peer Reviewed
• [Journal Article] Interleukin-6 (IL-6) Regulates Claudin-2 Expression and Tight Junction Permeability in Intestinal Epithelium (2011)
  • Author(s): Takuya Suzuki, Naho Yoshinaga, and Soichi Tanabe
  • Journal Title: J Biol Chem Volume: 286 Issue: 36 Pages: 31263-31271
  • DOI: 10.1074/jbc.m111.238147
  • Peer Reviewed
• [Journal Article] Role of flavonoids in intestinal tight junction regulation (2011)
  • Author(s): Takuya Suzuki, Hiroshi Hara
  • Journal Title: J Nutr Biochem Volume: 22 Issue: 5 Pages: 401-408
  • DOI: 10.1016/j.jnutbio.2010.08.001
  • Peer Reviewed
• [Journal Article] Kaempferol Enhances Intestinal Barrier Function through the Cytoskeletal Association and Expression of Tight Junction Proteins in Caco-2 Cells (2011)
  • Author(s): Takuya Suzuki, Soichi Tanabe, Hiroshi Hara
  • Journal Title: J Nutr Volume: 141 Issue: 1 Pages: 87-94
  • DOI: 10.3945/jn.110.125633
  • Peer Reviewed
• [Journal Article] Streptococcus thermophilus ST28 ameliorates colitis in mice partially by suppression of inflammatory Th17 cells (2011)
  • Author(s): Tasuku Ogita, Megumi Nakashima, Hidetoshi Morita, Yasuo Saito, Takuya Suzuki, Soichi Tanabe
  • Journal Title: J Biomed Biotechnol Volume: 2011 Issue: 1 Pages: 378417-378417
  • DOI: 10.1155/2011/378417
  • Peer Reviewed
• [Journal Article] Suppression of Th17 response by Streptococcus thermopbilus ST28 through induction of IFN-γ (2011)
  • Author(s): Tasuku Ogita, Yusuke Tanii, Hidetoshi Morita, Takuya Suzuki, Soichi Tanabe
  • Journal Title: Int J Mol Med Volume: 28 Pages: 817-822
  • DOI: 10.3892/ijmm.2011.755
  • Peer Reviewed
• [Journal Article] The Effects of chicken collagen hydrolysate on the circulatory system in subjects with mild hypertension or high-normal blood pressure.
  • Author(s): Kouguchi T, Ohmori T, Shimizu M, Takahata Y, Maeyama Y, Suzuki T, Morimatsu F and Tanabe S
  • Journal Title: Biosci Biotechnol Biochem Volume: (in press)
  • Peer Reviewed
• [Journal Article] 市販コラーゲンペプチド摂取による自然発症性高血圧ラットの血圧上昇抑制作用の比較
  • Author(s): 河口 友美, 岩井 浩二, 清水 宗茂, 大森 丘, 高畑 能久, 鈴木 卓弥, 森松 文毅, 田辺 創一.
  • Journal Title: 食品科学工学会誌 Volume: (in press)
  • Peer Reviewed
• [Journal Article] Chicken collagen hydrolysate-derived peptides inhibit tumor necrosis factor-α-induced inflammatory response in endothelial cells
  • Author(s): Kouguchi T, Ito A,Iwai K, Shimizu M, Takahata Y, Suzuki T, Morimatsu F, and Tanabe S.
  • Journal Title: Food Sci Technol Res Volume: (in press)
  • NAID: 10031054535
  • Peer Reviewed
• [Presentation] Intestinal barrier regulation by dietary polyphenols (2012)
  • Author(s): Suzuki T
  • Organizer: International society nutraceuticals and food factors 2012 annual conference.
  • Place of Presentation: Kona, USA
• [Presentation] ナリンゲニンは、タイトジャンクションタンパク質の局在と発現調節を介して、消化管上皮Caco-2細胞のバリア機能を高める (2012)
  • Author(s): 野田咲乃、田辺創一、鈴木卓弥
  • Organizer: 第66回日本栄養・食糧学会大会
  • Place of Presentation: 東北大学
• [Presentation] ナリンゲニンによる大腸バリア機能保護作用と炎症抑制作用の相互解析 (2012)
  • Author(s): 東知世、重白みづき、田辺創一、鈴木卓弥
  • Organizer: 第66回日本栄養・食糧学会大会
  • Place of Presentation: 東北大学
• [Presentation] ナリンゲニンは、タンパク質の局在と発現調節を介して、消化管上皮Caco-2細胞のバリア機能を高める (2012)
  • Author(s): 鈴木卓弥
  • Organizer: 日本栄養・食糧学会大会
• [Presentation] ナリンゲニンによる大腸バリア機能保護作用と炎症抑制作用の相互解析 (2012)
  • Author(s): 東知世
  • Organizer: 日本栄養・食糧学会大会
• [Presentation] ナリンゲニンによる大腸バリア機能保護作用と炎症抑制作用は、他のポリフェ ノール（ケルセチン、クルクミン、ヘスペレチン）よりも強い (2012)
  • Author(s): 重白みづき、野田咲乃、田辺創一、鈴木卓弥
  • Organizer: 2012年度日本農芸化学会大会
  • Place of Presentation: 京都女子大
• [Presentation] インターロイキン－６はクローディン２の発現上昇を介して 消化管上皮タイトジャンクション透過性を高める (2011)
  • Author(s): 鈴木 卓弥、田辺 創一
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
• [Presentation] INTERLEUKIN-6 REGULATES CLAUDIN-2 EXPRESSION AND TIGHT JUNCTION PERMEABILITY IN INTESTINAL EPITHELIUM (2011)
  • Author(s): Takuya Suzuki, Soichi Tanabe
  • Organizer: United European Gastroenterology Week 2011
  • Place of Presentation: Stockholm, Sweden
• [Book] Kaempferol: Chemistry, Natural Occurrences and Health Benefits. Suppressive effect of kaempferol on the intestinal permeability. (2013)
  • Author(s): Takuya Suzuki
  • Total Pages: 211
  • Publisher: Nova Science Publishers, Inc.
• [Book] Handbook on Flavonoids: Dietary Sources, Properties and Health Benefits. Regulation of intestinal barrier function by dietary flavonoids. (2012)
  • Author(s): Takuya Suzuki
  • Total Pages: 557
  • Publisher: Nova Science Publishers, Inc.
• [Book] 生命・食・環境のサイエンス, 第5章3節 食べものによる病気の予防 (2011)
  • Author(s): 鈴木卓弥
  • Publisher: 共立出版
• [Book] Handbook on Flavonoids: Dietary Sources, Properties and Health Benefits. Regulation of intestinal barrier function by dietary flavonoids. Editors: Kazuya Yamane and Yuudai Kato. (2011)
  • Author(s): Suzuki T.
  • Publisher: Nova Science Publishers, Inc
• [Book] Quercetin: Dietary Sources, Functions and Health Benefits. Supressive effect of quercetin on intestinal tight junction permeability. Editors: Taiki Chikamatsu and Yuudai Hida (2011)
  • Author(s): Suzuki T
  • Publisher: Nova Science Publishers, Inc
• [Book] Suppressive Effect Of Quercetin On Intestinal Tight Junction Permeability (2011)
  • Author(s): Takuya Suzuki
  • Total Pages: 12
  • Publisher: Nova Science Publishers, Inc.
• [Book] Regulation Of Intestinal Barrier Function By Dietary Flavonoids (2011)
  • Author(s): Takuya Suzuki
  • Total Pages: 20
  • Publisher: Nova Science Publishers, Inc.
• [Book] 食品機能学, 第2章1節 ミネラルの吸収促進成分,第2章2節 血糖上昇抑制成分
  • Author(s): 鈴木卓弥
  • Publisher: 三共出版(印刷中)
• [Book] Kaempferol: Chemistry, Natural Occurrences and Health Benefits. Suppressive effect of kaempferol on the intestinal permeability. Editor: Jennifer Ramirez
  • Author(s): Suzuki T
  • Publisher: Nova Science Publishers, Inc(In press)
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/douri/Suzuki%20group/Top%20page.html