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Isolation of the receptor gene to Pine Wood Nematode in Pinus thunbergii

Research Project

Project/Area Number 23780170
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Forest science
Research InstitutionForestry and Forest Products Research Institute

Principal Investigator

HIRAO Tomonori  独立行政法人森林総合研究所, 森林バイオ研究センター, 研究員 (90457763)

Co-Investigator(Renkei-kenkyūsha) WATANABE Atsushi  独立行政法人森林総合研究所, 林木育種センター育種部, 研究室長 (10360471)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsエリシター受容体遺伝子 / クロマツ / マツノザイセンチュウ / 抵抗性遺伝子 / 抵抗性 / 感受性
Research Abstract

To isolate receptor gene (LRR-gene) to pine wood nematode (PWN) in Pinus thunbergii, the comparative genomic approach was conducted using the sequence information of nematode resistance gene (NR-gene) in cultivated crop. The isolated candidate gene was evaluated by QRL (Quantitative Resistance Loci) analysis between genotype and phenotype using the resistant F1 family. As a result, the significant QTL was not detected at receptor genes isolated in this study, but one putative QTL affecting PWN resistance was detected on the linkage group 1.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (6 results)

All 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results)

  • [Journal Article] Characterization of resistance to pine wood nematode infection in Pinus thunbergii using suppression subtractive hybridization2012

    • Author(s)
      Hirao T., Fukatsu E., Watanabe A
    • Journal Title

      BMC Plantb biology

      Volume: 12 Issue: 1

    • DOI

      10.1186/1471-2229-12-13

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Comparison of gene expression profiles between resistance and susceptibility in Pinus thunbergii to pine wood nematode infection2012

    • Author(s)
      Hirao T., Watanabe A
    • Organizer
      IUFRO Working Unit 7.03.12"Alien invasive species and international trade"
    • Place of Presentation
      東京大学(東京都)
    • Related Report
      2012 Final Research Report
  • [Presentation] マツノザイセンチュウ抵抗性解明に向けたクロマツ生体防御に関連する遺伝子発現応答プロファイルの構築2012

    • Author(s)
      平尾知士, 渡辺敦史
    • Organizer
      第123回日本森林学会大会
    • Place of Presentation
      宇都宮大学(栃木県)
    • Related Report
      2012 Final Research Report
  • [Presentation] Defense response of resistance and susceptibility to pine wood nematode infection in Pinus thunbergii2012

    • Author(s)
      Hirao T., Fukatsu E., Watanabe A
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都府)
    • Related Report
      2012 Final Research Report
  • [Presentation] Detection of interspecific introgressive hybridization in Pinus using EST- SSR markers2012

    • Author(s)
      Hirao T., Shirasawa K., Tabata S., Watanabe A
    • Organizer
      Plant and animal genome XX
    • Place of Presentation
      サンディエゴ(アメリカ合衆国)
    • Related Report
      2012 Final Research Report
  • [Presentation] Comparison of gene expression profiles between resistance and susceptibility in Pinus thunbergii to pine wood nematode infection2012

    • Author(s)
      Tomonori Hirao
    • Organizer
      The work of IUFRO Unit 7.03.12 Alien invasive species and international trade
    • Place of Presentation
      東京大学(東京都)
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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