Why are male flower-specific genes of Cryptomeria japonica not expressed in callus?
| Project/Area Number |
23780174
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Forest science
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| Research Institution | Forestry and Forest Products Research Institute |
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Principal Investigator |
KURITA Manabu 独立行政法人森林総合研究所, 林木育種センター育種部, 室長 (40370829)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | スギ / エピジェネティクス / メチル化 / 遺伝子発現 |
|---|
| Research Abstract |
We tried to clarify the association between gene expression and methylation by analyzing the degree of methylation of the Muka14 promoter using bisulfite sequencing. We found that CpG sequences were methylated more than 1 kb upstream of the translation start point of the Muka14 gene, but were unmethylated downstream. The region required for male flower-specific expression of Muka14 is consistent with the distribution of unmethylated CpG. Our results suggest that the essential domains for the expression of target genes can be predicted using the methylation of promoters as an index.
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Report
(4 results)
Research Products
(8 results)
