Why are male flower-specific genes of Cryptomeria japonica not expressed in callus?

• Project/Area Number: 23780174
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Forest science
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: KURITA Manabu 独立行政法人森林総合研究所, 林木育種センター育種部, 室長 (40370829)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: スギ / エピジェネティクス / メチル化 / 遺伝子発現

Research Abstract

We tried to clarify the association between gene expression and methylation by analyzing the degree of methylation of the Muka14 promoter using bisulfite sequencing. We found that CpG sequences were methylated more than 1 kb upstream of the translation start point of the Muka14 gene, but were unmethylated downstream. The region required for male flower-specific expression of Muka14 is consistent with the distribution of unmethylated CpG. Our results suggest that the essential domains for the expression of target genes can be predicted using the methylation of promoters as an index.

Research Products

• [Journal Article] The promoter of an A9 homolog from the conifer Cryptomeria japonica imparts male strobilus-dominant expression in transgenic trees (2013)
  • Author(s): Manabu Kurita, Ken-ichi Konagaya, Atsushi Watanabe, Toru Taniguchi
  • Journal Title: Plant Cell Reports Volume: 32 Pages: 319-328
  • Peer Reviewed
• [Presentation] 遺伝子組換え技術による無花粉スギの作出と形質評価 (2013)
  • Author(s): 小長谷賢一、栗田学、坪村美代子、平尾知士、渡辺敦史、石井克明、谷口亨
  • Organizer: 森林遺伝育種学会
  • Place of Presentation: 東京大学
  • Year and Date: 2013-11-08
• [Presentation] Barnase-barstarシステムを用いた遺伝子組換え雄性不稔スギの作出と形質評価 (2013)
  • Author(s): 小長谷賢一、栗田学、坪村美代子、平尾知士、渡辺敦史、石井克明、谷口亨
  • Organizer: 植物細胞分子生物学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2013-09-10
• [Presentation] Isolation And Functional Analysis Of The Male Strobilus-Specific Promoters Of Cryptomeria japonica For Development Of The Male Sterility System (2013)
  • Author(s): Manabu Kurita, Ken-ichi Konagaya, Atsushi Watanabe, Toru Taniguchi
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学
  • Year and Date: 2013-03-21
• [Presentation] Barnase-barstarシステムを用いた 遺伝子組換え雄性不稔スギの作出と形質評価 (2013)
  • Author(s): 小長谷 賢一、栗田 学、坪村 美代子、平尾 知士、 渡辺 敦史、石井 克明、谷口 亨
  • Organizer: 植物細胞分子生物学会
  • Place of Presentation: 北海道
• [Presentation] Isolation And Functional Analysis Of The Male Strobilus-Specific Promoters Of Cryptomeria japonica For Development Of The Male Sterility System (2013)
  • Author(s): Manabu Kurita, Ken-ichi Konagaya, Atsushi Watanabe, Toru Taniguchi
  • Organizer: Annual Meeting of JSPP 2013
  • Place of Presentation: 岡山大学（岡山市）
• [Presentation] スギの雄花特異的に発現する遺伝子のプロモーターの機能解析 (2012)
  • Author(s): 栗田学、小長谷賢一、渡辺敦史、近藤禎二、石井克明、谷口亨
  • Organizer: 森林遺伝育種学会
  • Place of Presentation: 東京大学
  • Year and Date: 2012-11-08
• [Presentation] 遺伝子組換え技術による無花粉スギの作出と形質評価
  • Author(s): 小長谷 賢一、栗田 学、坪村 美代子、平尾 知士、渡辺 敦史、石井 克明、谷口 亨
  • Organizer: 森林遺伝育種学会
  • Place of Presentation: 東京