| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
In the present study, I tried to create the peptide which can bind to CCA-treated wood by using phage display library system as a first step for development of CCA-treated wood discriminating method. In the screening of the candidate peptide, the phage which has an ability to bind to CCA-treated wood was obtained. Based on the deduced amino acid sequence, the peptide attached FITC at C-terminal was synthesized chemically, and used for binding assay using CCA-treated and non-treated wood as target substances. As a result, the amount of the peptide bound to CCA-treated wood was significantly larger than that to non-treated wood. Moreover, the peptide was able to bind efficiently to CCA-treated wood, even when 1% CCA-treated wood, which is the concentration of use for building timber, was used. The results clearly suggests that the peptide could be used for the detection of CCA component in wood.
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