Elucidation of the selective lignin degradation mechanism in a white rot fungus and the biosynthetic pathway of its key metabolites through a proteomic approach
| Project/Area Number |
23780183
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Wood science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | リグニン / 白色腐朽菌 / 脂質関連代謝物 / プロテオーム |
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| Research Abstract |
A white-rot fungus, Ceriporiospsis subvermispora, can selectively degrade lignin without serious damage to cellulose. In order to elucidate the molecular mechanism of the selective lignin degradation, we tried to investigate the cellular response of C. subvermispora to a lignin fragment, vanillin. We obtained expression profiles of C. subvermispora in the presence and absence of vanillin through a proteomics-based approach, and detected vanillin-induced and repressed proteins. Using suppression subtractive hybridization, moreover, we also obtained many genes clones that are up-regulated in the presence of vanillin.
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Report
(4 results)
Research Products
(11 results)
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[Presentation] Cellular response of a selective lignin-degrading fungus to vanillin. Lignobiotech II Symposium2012
Author(s)
Watanabe, T., Mori, K., Kido, A., Izumi, C., Hasegawa, T., Tsuda, S., Nishimura, H., Honda, Y., Watanabe, T
Organizer
2nd Symposium on Biotechnology Applied to Ligonocelluloses
Place of Presentation
Fukuoka
Related Report
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