| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Research Abstract |
To understand molecular mechanisms behind crystallization processes in cellulose biosynthesis, we analyzed the two cellulose synthases in the marine chordate Oikopleura dioica, those are proposed to be involved in the formation of two polymorphs of cellulose I. With negative results in assessing the embryos injected with mRNAs encoding mutated cellulose synthases, we challenged to express native forms using heterologous system with bacterial, yeast, and insect cell hosts.
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