| Project/Area Number |
23780195
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General fisheries
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| Research Institution | Ehime University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAYAMA Kei 愛媛大学, 沿岸環境科学研究センター, 助教 (80380286)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | リンホシスチス病 / リンホシスチスウイルス |
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| Research Abstract |
Lymphocystis disease virus (LCDV) is the causative agent of lymphocystis disease (LCD). In this study, we investigated the mechanisms of lymphocystis cell (LCC) formation in the fin of the fish infected with LCDV by microarray experiments. LCCs firstly appeared in the fish at 21 days post infection (dpi). The microarray detected a few gene expression changes until 28 dpi. However, the number of expression changed genes dramatically increased between 28 and 42 dpi in which LCCs formation was active. From the microarray data analyses, apoptosis-related genes and cyclin-dependent kinase (CDK) 1 gene were down-regulated, whereas cell fusion and collagen related genes were up-regulated at 42 dpi. Together with the observation of morphological changes of LCCs in previous reports, it is suggested that the following steps are involved in LCC formation: the virus infected cells(1) experienced inhibited apoptotic death before enlargement,(2) experienced inhibited cell division by G2/M cell cycle arrest,(3) were hypertrophied by cell fusion, and(4) were surrounded by a hyaline capsule associated with the alteration of collagen fibers.
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