| Project/Area Number |
23780202
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General fisheries
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| Research Institution | Dokkyo Medical University |
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Principal Investigator |
NONAKA Lisa 獨協医科大学, 医学部, 助教 (70363265)
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| Co-Investigator(Renkei-kenkyūsha) |
MARUYAMA Fumito 京都大学, 医学部, 准教授 (30423122)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | pAQU group / 薬剤耐性 / 多剤耐性 / プラスミド / 遺伝子伝達 / マクロライド / mef(C)-mph(G) / 養殖場 / マクロライド耐性遺伝子 / リン酸化酵素 / 排出ポンプ / mef(C) / mph(G) / 多剤耐性菌 / 耐性遺伝子 / 伝達性プラスミド / 養殖環境 / pAQU2 / tet(M) / ICE / 薬剤耐性遺伝子 / 遺伝子伝播 / トランスポゾン |
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| Outline of Final Research Achievements |
It was found 4 strains isolated from aquaculture environment possessed transferable multiple drug resistance plasmid which were similar to autonomously transferable plasmid, pAQU1, carried by a strain of Photobacterium damselae subsp. damselae 04Ya311. They ranged 150-350 kb and shared a ~100-kbp highly conserved region, suggesting that these plasmids constituted “pAQU group”. The results of the present study indicate that the pAQU group plasmids may play an important role in dissemination of ARGs in the marine environment.
Additionally, mef(C) and mph(G) which had similarity to efflux pump and phosphotransferase, respectively were found to be novel macrolide resistance genes. These genes were encoded head-on-tail on pAQU1 and it was conserved on plasmids ranging 240-350 kb of the 22 erythromycin-resistant strains belonging to Vibrio and Photobacteirum. This is a first report of macrolide-resistance genes originating from a marine environment.
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