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Risk of multi-drug resistance transfer via plasmids from aquaculture environment to human

Research Project

Project/Area Number 23780202
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General fisheries
Research InstitutionDokkyo Medical University

Principal Investigator

NONAKA Lisa  獨協医科大学, 医学部, 助教 (70363265)

Co-Investigator(Renkei-kenkyūsha) MARUYAMA Fumito  京都大学, 医学部, 准教授 (30423122)
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
KeywordspAQU group / 薬剤耐性 / 多剤耐性 / プラスミド / 遺伝子伝達 / マクロライド / mef(C)-mph(G) / 養殖場 / マクロライド耐性遺伝子 / リン酸化酵素 / 排出ポンプ / mef(C) / mph(G) / 多剤耐性菌 / 耐性遺伝子 / 伝達性プラスミド / 養殖環境 / pAQU2 / tet(M) / ICE / 薬剤耐性遺伝子 / 遺伝子伝播 / トランスポゾン
Outline of Final Research Achievements

It was found 4 strains isolated from aquaculture environment possessed transferable multiple drug resistance plasmid which were similar to autonomously transferable plasmid, pAQU1, carried by a strain of Photobacterium damselae subsp. damselae 04Ya311. They ranged 150-350 kb and shared a ~100-kbp highly conserved region, suggesting that these plasmids constituted “pAQU group”. The results of the present study indicate that the pAQU group plasmids may play an important role in dissemination of ARGs in the marine environment.
Additionally, mef(C) and mph(G) which had similarity to efflux pump and phosphotransferase, respectively were found to be novel macrolide resistance genes. These genes were encoded head-on-tail on pAQU1 and it was conserved on plasmids ranging 240-350 kb of the 22 erythromycin-resistant strains belonging to Vibrio and Photobacteirum. This is a first report of macrolide-resistance genes originating from a marine environment.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (9 results)

All 2015 2014 2013 2012 2011

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Novel macrolide resistance genes, mef(C) and mph(G) carried by plasmids from Vibrio and Photobacterium isolated from sediment and seawater of a coastal aquaculture site2015

    • Author(s)
      Lisa Nonaka, Fumito Maruyama, Satoru Suzuki, Michiaki Masuda
    • Journal Title

      Letters in Applied Microbiology

      Volume: 印刷中

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Various pAQU plasmids possibly contribute to disseminate tetracycline resistance gene tet(M) among marine bacterial community.2014

    • Author(s)
      Nonaka, L., Maruyama, F., Onishi, Y., Kobayashi, T., Ogura, Y., Hayashi, T., Suzuki, S. and Masuda, M.
    • Journal Title

      Frontiers in Microbiology

      Volume: 5 Pages: 1-12

    • DOI

      10.3389/fmicb.2014.00152

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel Conjugative Transferable Multiple Drug Resistance Plasmid pAQU1 from <i>Photobacterium damselae</i> subsp. <i>damselae</i> Isolated from Marine Aquaculture Environment2012

    • Author(s)
      Lisa Nonaka
    • Journal Title

      Microbes and Environments

      Volume: 27 Issue: 3 Pages: 263-272

    • DOI

      10.1264/jsme2.ME11338

    • NAID

      10030869338

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Novel conjugative transferable multiple drug resistance plasmid pAQU1 from Photobacterium damselae subsp. damselae isolated from marine aquaculture environment2011

    • Author(s)
      Lisa Nonaka
    • Journal Title

      Microbes and environments

      Volume: in press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 養殖環境における抗菌薬耐性菌2014

    • Author(s)
      野中里佐
    • Organizer
      日本防菌防黴学会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-24 – 2014-09-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Photobacterium damselae subsp. damselaeが保有する多剤耐性プラスミド由来、新規マクロライド耐性遺伝子mef(C), mph(G)2014

    • Author(s)
      野中里佐、丸山史人、鈴木 聡、増田道明
    • Organizer
      日本感染症学会、日本化学療法学会合同学会
    • Place of Presentation
      福岡
    • Year and Date
      2014-06-18 – 2014-06-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Novel macrolide resistance operon, mef(C)-mph(G), carried by plasmids isolated from Vibrio and Photobacterium species from an aquaculture site2013

    • Author(s)
      Lisa Nonaka, Fumito Maruyama, Satoru Suzuki, Michiaki Masuda
    • Organizer
      日本微生物生態学会
    • Place of Presentation
      鹿児島
    • Related Report
      2013 Research-status Report
  • [Presentation] 多剤耐性Photobacterium damselae subsp. damselae由来新規マクロライド耐性遺伝子mef(C)およびmph(G)のクローニングと沿岸養殖環境細菌における分布2013

    • Author(s)
      野中 里佐、丸山史人、鈴木聡、増田道明
    • Organizer
      第42回薬剤耐性菌研究会
    • Place of Presentation
      静岡
    • Related Report
      2013 Research-status Report
  • [Presentation] 養殖環境に分布する多剤耐性伝達性プラスミドの比較解析2011

    • Author(s)
      野中里佐
    • Organizer
      日本微生物生態学会第27回大会
    • Place of Presentation
      京都
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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