| Project/Area Number |
23780273
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
HAGI Tatsuro 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所・畜産物研究領域, 研究員 (00510257)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 微生物 / ウシ乳腺上皮細胞 / マイクロアレイ / 牛乳 / 乳腺上皮細胞 |
|---|
| Research Abstract |
In this study, we investigated the effect of predominant bacteria in bovine milk on bovine mammary gland epithelial cells (BMEC) which could recognize bacteria. Microarray analysis of gene expression in BMEC in response to the most predominant milk bacteria Lactococcus lactis showed that change in gene expression related to intracellular signaling pathway was observed after treatment of L. lactis. The real time PCR analysis of gene expression in BMEC in response to gram-positive bacteria (3 strains including L. lactis) and gram-negative bacteria (4 strains) showed that gram-negative bacteria strongly enhanced gene expression of inflammatory cytokines. Furthermore, the effect of predominant milk bacteria on signaling pathway in BMEC was different among species.
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