The elucidation of the apoptotic mechanism in cryopreserved bovine oocytes and the establishment of the new method for cryopreservation and in vitro culture
Project/Area Number |
23780278
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied animal science
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Research Institution | Yamagata University |
Principal Investigator |
ABE Yasuyuki 山形大学, 理工学研究科, 助教 (80447086)
|
Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
|
Keywords | 発生工学 / ウシ / 卵子 / 凍結保存 / アポトーシス / ミトコンドリア / 卵母細胞 / 卵核胞期卵子 / 細胞骨格 |
Research Abstract |
For the purpose of the improvement of the cryopreservation method in bovine oocytes (germinal vesicle stage), we analyzed the effect of the cryopreservation on oocytes. The cryopreservation changed mitochondrial and microtublar localization, as a result, mitochondrial functional decline and induction of the apoptosis occurred. Then, we were successful in increasing the rates of maturation, fertilization and development in the cryopreserved oocytes by the treatment of the cytoskeleton stabilizer and removal of the cumulus cells. Also, for the purpose of evading a fertilization disorder of the cryopreserved oocytes, we analyzed of the factor which controlled fertility of the sperm. We found that linearity and velocity of moving, and acrosomal normality had influence to fertility of the sperm.
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] 2,6- Bis(arylsulfonyl)anilines as Fluorescent Scaffolds through Intramolecular Hydrogen Bonds : Solid- State Fluorescence Materials and Turn-On-Type Probes Based on Aggregation-Induced Emission2014
Author(s)
Beppu, T., Kawata, S., Aizawa, N., Pu,Y. J., Abe, Y., Ohba, Y., Katagiri, H.
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Journal Title
ChemPlusChem
Volume: 79 (4)
Pages: 536-545
Related Report
Peer Reviewed
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[Journal Article] 2,6-Bis(arylsulfonyl)anilines as Fluorescent Scaffolds through Intramolecular Hydrogen Bonds : Solid-State Fluorescence Materials and Turn-On-Type Probes Based on Aggregation-Induced Emission2014
Author(s)
Beppu, T., Kawata, S., Aizawa, N., Pu, Y. -J., Abe, Y., Ohba, Y., Katagiri
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Journal Title
ChemPlusChem
Volume: 79
Issue: 4
Pages: 536-545
DOI
Related Report
Peer Reviewed
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[Journal Article] Distribution of artificial radionuclides in abandoned cattle in theeva cuation zone of the Fukushima Daiichi nuclear power plant2013
Author(s)
Fukuda T, Kino Y, Abe Y, Yamashiro H, Kuwahara Y, Nihei H, Sano Y, Irisawa A, Shimura T, Fukumoto M, Shinoda H, Obata Y, Saigusa S, Sekine T, Isogai E, Fukumoto M.
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Journal Title
PLoS One
Volume: 8(1)
Issue: 1
Pages: 1-7
DOI
Related Report
Peer Reviewed
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[Journal Article] Dynamics of intracellular phospholipid membrane organization during oocyte maturation and successful vitrification of immature oocytes retrieved by ovum pick-up in cattle2013
Author(s)
Aono, A., Nagatomo, H., Takuma, T., Nonaka, R., Ono, Y., Wada, Y., Abe, Y., Watanabe, T., Kawahara, M.
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Journal Title
Theriogenology
Volume: 79
Issue: 8
Pages: 1146-1152
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Effects of radioactive caesium on bull testes after the Fukushima nuclear plant accident2013
Author(s)
Yamashiro, H., Abe, Y., Fukuda, T., Kino, Y., Kuwahara, Y., Fukumoto, M., Kobayashi, J., Uematsu, E., Tong, B., Sugiyama, T., Yamada, T., Shinoda, H., Sekine, T., Sato, E.
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Journal Title
Sci. Rep.
Volume: (掲載決定)
Issue: 1
Pages: 2850-2856
DOI
Related Report
Peer Reviewed
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[Journal Article] Transcriptional wiring for establishing cell lineage specification at the blastocyst stage in cattle2013
Author(s)
Nagatomo, H., Kagawa, S., Kishi, Y., Takuma, T., Sada, A., Yamanaka, K., Abe, Y., Wada, Y., Takahashi, M., Kono, T., Kawahara, M.
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Journal Title
Biol. Reprod
Volume: Jun 27;88(6)
Issue: 6
Pages: 158-158
DOI
Related Report
Peer Reviewed
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[Journal Article] Distribution of Radioactive Cesium in Ostrich (Struthio camelus) after the Fukushima Daiichi Nuclear Power Plant Accident.2013
Author(s)
Isogai, E., Kino, Y., Abe, Y., Yamashiro, H., Shinoda, H., Fukuda, T., Fukumoto, M., Kuroda, K., Yoneyama, H., Isogai, E., Sekine, T.
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Journal Title
Radiat. Emerg. Med.
Volume: 2
Pages: 68-71
NAID
Related Report
Peer Reviewed
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[Journal Article] 福島第一原子力発電所事故に伴う被災家畜の臓器別放射性セシウム濃度2012
Author(s)
福本学,被災動物放射能評価グループ(磯貝恵美子,福田智一,木野康志,関根勉,篠田壽,桑原義和,志村勉,福本基,阿部靖之,山城秀昭,三枝新,小幡裕一)
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Journal Title
Isotope News
Volume: 696(4)
Pages: 10-12
NAID
URL
Related Report
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[Journal Article] 警戒区域で何が起こっているか?牛における放射性物質の動態2012
Author(s)
福本学,磯貝恵美子,福田智一,木野康志,関根勉,篠田壽,千葉美麗,鈴木敏彦,清水央,高橋温,西村真,桑原義和,志村勉,鈴木正敏,井上和也,高橋慎太郎,福本基,阿部靖之,山城秀昭,小林仁,森本素子,三枝新,小幡裕一
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Journal Title
家畜衛生学雑誌
Volume: 38
Pages: 105-106
Related Report
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[Journal Article] Cryopreservation of canine embryo2011
Author(s)
Abe, Y., Suwa, Y., Asano, T., Yanagimoto- Ueta, Y., Kobayashi, N., Ohshima, N., Shirasuna, S., Abdel-Ghani, M. A., Oi, M., Kobayashi, Y., Miyoshi, M., Miyahara, K., Suzuki, H.
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Journal Title
Biol. Reprod.
Volume: 84(2)(Epub 2010 Oct 6)
Issue: 2
Pages: 363-368
DOI
Related Report
Peer Reviewed
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[Journal Article] Distribution of Radioactive Cesium in Ostrich (Struthio camelus) after the Fukushima Daiichi Nuclear Power Plant Accident
Author(s)
Isogai, E., Kino, Y., Abe, Y., Yamashiro, H., Shinoda, H., Fukuda, T., Fukumoto, M., Kuroda, K., Yoneyama, H., Isogai, E., Sekine, T.
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Journal Title
Radiat. Emerg. Med.
Volume: (掲載決定)
NAID
URL
Related Report
Peer Reviewed
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[Presentation] Effects of radiation on abandoned bull associated with Fukushirna Nuclear Power Plant accident2013
Author(s)
Yamashiro, H., Abe, Y., Kuwahara, Y., Urushihara, Y., Suzuk, M., Inoue, K., Takahash, S., Kino, Y., Fukuda, T., Isogai, E., Fukumoto, M.
Organizer
CABX Workshop and international Symposium
Place of Presentation
(Jeiu,Korea)
Related Report
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[Presentation] 福島第一原発20km圏内の雌ウシにおける卵巣の正常性2013
Author(s)
阿部靖之,山城秀昭,福田智一,木野康志,関根勉,桑原義和,福本基,小林仁,阿部宏之,磯貝恵美子,福本学
Organizer
第56回日本放射線影響学会
Place of Presentation
(青森)(日講演要旨集p37)
Related Report
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[Presentation] 福島第一原発20km圏内に生息する雌ウシの生殖器に対する放射性物質の影響解析2013
Author(s)
阿部靖之,山城秀昭,及川俊徳,福田智一,木野康志,関根勉,桑原義和,福本基,小林仁,阿部宏之,沼邊孝,磯貝恵美子,福本学
Organizer
第54回日本哺乳動物卵子学会
Place of Presentation
(東京)(講演要旨集p13)
Related Report
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[Presentation] Effect of vitrification at GV stage on the mitochondrial and cytoskeletal integrity in bovine oocytes2012
Author(s)
Abe, Y., Takakura, K., Kaito, K., Ogawa, T., Yokoo, M., Abe, H.
Organizer
38th Annual Conference of the IETS
Place of Presentation
(Phoenix, USA)
Related Report
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