The mechanism for the hypoglycaemia induced suppression of gonadal function in the pituitary gland

• Project/Area Number: 23780282
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied animal science
• Research Institution: Kinki University
• Principal Investigator: MORIYAMA Ryutaro 近畿大学, 理工学部, 講師 (30411573)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 低血糖 / LH / FSH / AMPK / 下垂体 / 性腺刺激ホルモン産生細胞 / 性腺機能 / 低栄養 / ゴナドトロフ / 性腺刺激ホルモン / AICAR / 栄養 / 血糖値 / 生殖 / GnRH / グルコース

Research Abstract

In the present study, we observed the restriction of glucose availability induced the transcriptional suppression of luteinizing hormone beta subunit (LHbeta) and follicle-stimulating hormone beta subunit (FHSbeta) genes via AMP-activated protein kinase (AMPK) signaling pathway in the gonadotropic LbetaT2 cell line. The result suggests a possibility that gonadotrophs of the pituitary gland participate in nutritional disorders inducing reproductive dysfunction in many mammalian species from cattle to humans.

Research Products

• [Journal Article] Redundancy in Kiss1 Expression Safeguards Reproduction in the Mouse (2013)
  • Author(s): S. M. Popa, R. M. Moriyama, C. S. Caligioni, C. M. Cho, T. L. Concepcion, A. E. Oakley, I. H. Lee, E. Sanz, P. S. Amieux, A. Caraty, R. D. Palmiter, V. M. Navarro, Y.-M. Chan, S. B. Seminara, D. K. Clifton, A. Steiner
  • Journal Title: Endocrinology Volume: Vol. 154, Issue 8 Pages: 2784-2794
  • Peer Reviewed
• [Journal Article] Redundancy in Kiss1 Expression Safeguards Reproduction in the Mouse. (2013)
  • Author(s): Simina M. Popa, Ryutaro M. Moriyama, Claudia S. Caligioni, Caroline M. Cho, Tessa L. Concepcion, Amy E. Oakley, In Hae Lee, Elisenda Sanz, Paul S. Amieux, Alain Caraty, Richard D. Palmiter, Victor M. Navarro, Yee-Ming Chan, Stephanie B. Seminara, Donald K. Clifton, Robert A. Steiner
  • Journal Title: Endocrinology Volume: 154(8) Issue: 8 Pages: 2784-2794
  • DOI: 10.1210/en.2013-1222
  • Peer Reviewed
• [Journal Article] Fukushima, Lysophosphatidic acid induces neurite branch formation through LPA3 (2012)
  • Author(s): D. Furuta, M. Yamane, T. Tsujiuchi, R. Moriyama, and N.
  • Journal Title: Molecular and Cellular Neuroscience Volume: Vol.50, Issue 1 Pages: 21-34
  • Peer Reviewed
• [Journal Article] Lysophosphatidic acid induces neurite branch formation through LPA_3 (2012)
  • Author(s): Furuta, D., et al
  • Journal Title: Mol.Cell.Neurosci. Volume: (印刷中) Issue: 1 Pages: 21-34
  • DOI: 10.1016/j.mcn.2012.03.006
  • Peer Reviewed
• [Presentation] 長鎖脂肪酸への暴露がマウス精子の運動性に与える影響 (2013)
  • Author(s): 森山隆太郎、若狭郁美、中川雅子
  • Organizer: 第106回日本繁殖生物学会大会
  • Place of Presentation: 府中、東京農工大学
  • Year and Date: 2013-09-13
• [Presentation] マウス下垂体のα-subunit含有細胞に局在するリゾホスファチジン酸受容体1 mRNAの発現量は去勢により増加する (2013)
  • Author(s): 森山隆太郎,松本絵美,原玲奈,宮里公子,十河由紀,福嶋伸之
  • Organizer: 第28回下垂体研究会学術集会
  • Place of Presentation: 花巻温泉、ホテル千住閣
  • Year and Date: 2013-08-07
• [Presentation] A Peripherally-Restricted Kappa Agonist (PRKA) as a Novel Approach to the Treatment of Menopausal Hot Flashes (2013)
  • Author(s): A. E. Oakley, R. Million, L. W. Organick, R. Moriyama, K. E. Manbeck, J. R. Levin, D. Reed, C. Chavkin, D. K. Clifton, R.t A. Steiner
  • Organizer: Endocrine Society's 95^<th> Annual Meeting & Expo (ENDO2013)
  • Place of Presentation: San Francisco, California
  • Year and Date: 2013-06-17
• [Presentation] 長鎖脂肪酸への暴露がマウス精子の運動性に与える影響 (2013)
  • Author(s): 森山隆太郎、若狭郁美、中川雅子
  • Organizer: 第106回日本繁殖生物学会大会
  • Place of Presentation: 東京農工大学
• [Presentation] マウス下垂体のα-subunit含有細胞に局在するリゾホスファチジン酸受容体1 mRNAの発現量は去勢により増加する (2013)
  • Author(s): 森山隆太郎，松本絵美，原玲奈，宮里公子，十河由紀，福嶋伸之
  • Organizer: 第28回下垂体研究会学術集会
  • Place of Presentation: 花巻温泉・ホテル千住閣
• [Presentation] A Peripherally-Restricted Kappa Agonist (PRKA) as a Novel Approach to the Treatment of Menopausal Hot Flashes. (2013)
  • Author(s): Amy E. Oakley, Rakeb Million, Lee W. Organick, Ryutaro Moriyama, Katherine E. Manbeck, Jamie R. Levin, Susan D. Reed, Charles Chavkin, Don K. Clifton, Robert A. Steiner
  • Organizer: ENDO2013
  • Place of Presentation: San Francisco
• [Presentation] Metabolic Regulation of Reproduction : The Effects of AgRP Neuronal Ablation on Kiss1 Expression in Leptin-Deficient Mice (2012)
  • Author(s): R. Million, A. E. Oakley, R. Moriyama, D.K. Clifton, R.A. Steiner
  • Organizer: 2012 Annual Biomedical Research Conference for Minority Students (ABRCMS)
  • Place of Presentation: San Jose, California(B049)
  • Year and Date: 2012-11-08
• [Presentation] Fertility in the Presence of Minute Levels of Kisspeptin (2012)
  • Author(s): S. M. Popa, R. M. Moriyama, C. Cho, T. L. Concepcion, A. E. Oakley, I. H. Lee, E. Sanz, C. S. Caligioni, Yee-Ming Chan, S. B. Seminara, V. M. Navarro, D. K Clifton, R. A. Steiner
  • Organizer: 2^<nd> World Conference of Kisspeptin signaling in the brain
  • Place of Presentation: University of Tokyo, Tokyo
  • Year and Date: 2012-11-07
• [Presentation] The role of kisspeptin in the restriction of fertility following ablation of Agouti-related peptide (AGRP) neurons in the leptin-deficient mice (2012)
  • Author(s): A. E. Oakley, I. H. Lee, R. Moriyama, R. Million, R. D. Palmiter, D. K. Clifton, R. A. Steine
  • Organizer: 2^<nd> World Conference of Kisspeptin signaling in the brain
  • Place of Presentation: University of Tokyo, Tokyo
  • Year and Date: 2012-11-07
• [Presentation] The requirement of Kiss1 for reproduction is sexually differentiated (2012)
  • Author(s): S. Popa, R. Moriyama, A. Oakley, C. Cho, M. Gottsch, T. Conception, D. Clifton, R. Steiner
  • Organizer: Northwest developmental biology meeting
  • Place of Presentation: Friday harbor laboratories, Washington
  • Year and Date: 2012-03-15
• [Presentation] The Effects of AgRP Neuronal Ablation on Kiss1 Expression in Leptin-Deficient Mice. (2012)
  • Author(s): R. Million, A.E. Oakley, R. Moriyama, D.K. Clifton, R.A. Steiner
  • Organizer: Annual Biomedical Research Conference for Minority Students (ABRCMS)
  • Place of Presentation: San Jose, California
• [Presentation] Fertility in the Presence of Minute Levels of Kisspeptin. (2012)
  • Author(s): S.M. Popa, R.M. Moriyama, C. Cho, T.L. Concepcion, A.E. Oakley, I.H. Lee, E. Sanz, C.S. Caligioni, Yee-Ming Chan, S.B. Seminara, V.M. Navarro, D.K Clifton, R.A. Steiner
  • Organizer: 2nd World Conference of Kisspeptin signaling in the brain
  • Place of Presentation: University of Tokyo, Tokyo
• [Presentation] The role of kisspeptin in the restriction of fertility following ablation of Agouti-related peptide (AGRP) neurons in the leptin-deficient mice. (2012)
  • Author(s): A.E. Oakley, I.H. Lee, R. Moriyama, R. Million, R.D. Palmiter, D.K. Clifton, R.A. Steiner
  • Organizer: 2nd World Conference of Kisspeptin signaling in the brain
  • Place of Presentation: University of Tokyo, Tokyo
• [Presentation] The requirement of Kiss1 for reproduction is sexually differentiated. (2012)
  • Author(s): S.M. Popa, R. Moriyama, A.E. Oakley, C. Cho, M. Gottsch, T.L. Conception, D.K. Clifton, R.E. Steiner
  • Organizer: Northwest developmental biology meeting
  • Place of Presentation: Friday harbor laboratories, Washington
• [Presentation] Kiss1 for reprod is sexually differentiated. (2012)
  • Author(s): S. Popa
  • Organizer: Northwest Developmental Biology Meeting
  • Place of Presentation: Friday Harbor Laboratories
• [Presentation] Gonadal steroids down-regulate the long-chain fatty acid receptor GPR120 mRNA expression levels in gonadotrophs of the mouse anterior pituitary gland (2011)
  • Author(s): R. Moriyama, M. Toyonaga, T. Miyazato, Y. Sogo, N. Fukushima
  • Organizer: 44th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Oregon Convention Center, Portland, Oregon, U.S.A.
  • Year and Date: 2011-08-02
• [Presentation] Lysophosphatidic acid signaling is involved in neurite branch formation (2011)
  • Author(s): N. Fukushima, D. Furuta, M. Yamane, T. Tsujiuchi, R. Moriyama
  • Organizer: The 10th International conference on Alzheimer's and Parkinson's diseases (AD/PD 2011)
  • Place of Presentation: Barcelona, Spain
• [Presentation] Gonadal steroids regulate GPR120 expression. (2011)
  • Author(s): R. Moriyama
  • Organizer: The Society for the Study of Reproduction
  • Place of Presentation: Oregon Convention Center
• [Remarks]
  • URL: http://www.life.kindai.ac.jp/~mory/index.html