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Identification and analysis of chondroitin sulfates which regulate bone remodeling, and control of bone mass

Research Project

Project/Area Number 23780293
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionTottori University

Principal Investigator

HOSAKA Yoshinao  鳥取大学, 農学部, 教授 (00337023)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords破骨細胞 / 骨芽細胞 / コンドロイチン硫酸 / 骨リモデリング / 分化 / RANKL / RANK / 骨吸収能 / 骨形成と骨分解 / 骨粗鬆症 / OVXマウス / M-CSF / 水晶電子マイクロバランス法 / 細胞の分化 / CS-E / CS-0 / ハウシップ窩
Research Abstract

In present study, I investigated the osteoclast inhibition ability in CS and the mechanism how CS inhibit osteoclast differentiation in vitro. CS was significantly inhibited the number of tartrate-resistance acid phosphatase (TRAP)-positive multinucleated cells and bone resorption activity in pit formation assay.In quartz-crystal microbalance method, CS revealed the binding ability to RANKL and interrupted binding of RANK to RANKL. Furthermore, CS reduced the phosphorylation of extracellular ERK in pre-osteoclast cells. These results indicate that CS suppress osteoclast size and osteoclastogenesis by binding to RANKL and inhibited RANK signal pathway.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (11 results)

All 2013 2012 2011 Other

All Journal Article (1 results) Presentation (8 results) Remarks (2 results)

  • [Journal Article] Diamond squid (Thysanoteuthis rhombus)-derived chondroitin sulfate stimulates bone healing within a rat calvarial defect2013

    • Author(s)
      Hosaka YZ, Iwai Y, Tamura J, Uehara M
    • Journal Title

      Marine Drugs

      Volume: 11 Pages: 5024-5035

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 破骨細胞の分化と骨吸収能をコンドロイチン硫酸は抑制する2013

    • Author(s)
      近藤竜明、保坂善真、田村純一、上原正人
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜市
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] コンドロイチン硫酸の骨形成能生体内での検証2013

    • Author(s)
      朝倉ひとみ、保坂善真、田村純一、上原正人
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜市
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] コンドロイチン硫酸の骨形成能:生体内での検証2013

    • Author(s)
      朝倉ひとみ、保坂善真、田村純一、上原正人
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学(岐阜市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 破骨細胞の分化と骨吸収能をコンドロイチン硫酸は抑制する2013

    • Author(s)
      近藤竜明、保坂善真、田村純一、上原正人
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学(岐阜市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Sulfate group on chondroitin sulfate makes a different influence on osteoclast differentiation and function2012

    • Author(s)
      KONDO Tatsuaki, HOSAKA Yoshinao Z, UEHARA Masato
    • Organizer
      The 4th Congress of the Asian Association of Veterinary Anatomists
    • Place of Presentation
      Phuket, Thailand
    • Related Report
      2012 Research-status Report
  • [Presentation] Osteogenic potential of chondroitin sulfate-E with collagen gel scaffolds2012

    • Author(s)
      ASAKURA Hitomi, HOSAKA Yoshinao Z, UEHARA Masato
    • Organizer
      The 4th Congress of the Asian Association of Veterinary Anatomists
    • Place of Presentation
      Phuket, Thailand
    • Related Report
      2012 Research-status Report
  • [Presentation] コンドロイチン硫酸の糖鎖構造が決定する細胞分化の方向性2012

    • Author(s)
      保坂善真、田村純一、一柳剛、上原正人
    • Organizer
      第153回日本獣医学会学術集会(招待講演)
    • Place of Presentation
      大宮ソニックシティ(大宮市)
    • Related Report
      2011 Research-status Report
  • [Presentation] コンドロイチン硫酸の骨形成能について2011

    • Author(s)
      岩井勇次、保坂善真、田村純一、上原正人
    • Organizer
      第152回日本獣医学会学術集会
    • Place of Presentation
      大阪府立大学(堺市)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://staff.muses.tottori-u.ac.jp/

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://staff.muses.tottori-u.ac.jp/y-hosa/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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