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Involvement of MARCKS on methylmercury neurotoxicity and application as a marker for neurotoxicity

Research Project

Project/Area Number 23780298
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionKagoshima University

Principal Investigator

SHIRAISHI Mitsuya  鹿児島大学, 獣医学部, 准教授 (20383656)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsメチル水銀 / MARCKS
Research Abstract

Because myristoylated alanine-rich C kinase substrate (MARCKS) plays an essential role in the differentiation and development of neuronal cells, we studied the alteration of MARCKS expression and phosphorylation in MeHg-induced neurotoxicity of neuroblastoma SH-SY5Y cells and in the rat brain. Exposure to MeHg induced a decrease in cell viability of SH-SY5Y cells, which was accompanied by an alteration of MARCKS phosphorylation and expression. In brain tissue from MeHg-treated rats, abnormal MARCKS phosphorylation was observed. The present study may indicate that alteration in MARCKS has consequences for MeHg-induced neurotoxicity.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (15 results)

All 2014 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (12 results)

  • [Journal Article] Alteration in MARCKS phosphorylation and expression by methylmercury in SH-SY5Y cells and rat brain2014

    • Author(s)
      Mitsuya Shiraishi, Makoto Hangai, Megumi Yamamoto, Masanori Sasaki, Atsuhiro Tanabe, Yasuharu Sasaki, Atsushi Miyamoto
    • Journal Title

      Environ Toxicol. Pharmacol.

      Volume: 37 Pages: 1256-1263

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] MARCKS dephosphorylation is involved in bradykinin-induced neurite outgrowth in neuroblastoma SH-SY5Y cells.2012

    • Author(s)
      Tanabe A, Shiraishi M, Negishi M, Saito N, Tanabe M, and Sasaki Y.
    • Journal Title

      J Cell Physiol.

      Volume: 227 Issue: 2 Pages: 618-629

    • DOI

      10.1002/jcp.22763

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Increased expression of aquaporin-4 with methylmercury exposure in the brain of the common marmoset2012

    • Author(s)
      Yamamoto, M., Takeya, M., Ikeshima-Kataoka, H., Yasui, M., Kawasaki, Y., Shiraishi, M., Majima, E., Shiraishi, S., Uezono, Y., Sasaki, M., Eto, K.
    • Journal Title

      The Journal of Toxicological Sciences

      Volume: 37 Issue: 4 Pages: 749-763

    • DOI

      10.2131/jts.37.749

    • NAID

      10030502324

    • ISSN
      0388-1350, 1880-3989
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Analysis of intracellular calcium mobilization induced by heavy metals using fura-2 and TPEN in SH-SY5Y cells2014

    • Author(s)
      大久保正人,宮本篤,白石光也
    • Organizer
      第87回日本薬理学会年会
    • Place of Presentation
      仙台市
    • Year and Date
      2014-03-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Alteration of MARCKS Expression and Phosphorylation Levels by Methylmercury2013

    • Author(s)
      Mitsuya Shiraishi, Makoto Hangai, Megumi Yamamoto, Atsuhiro Tanabe, Atsushi Miyamoto
    • Organizer
      The XIII International Congress of Toxicology
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2013-07-02
    • Related Report
      2013 Final Research Report
  • [Presentation] 重金属による細胞内カルシウム濃度の変化とその特徴2013

    • Author(s)
      大久保正人,宮本篤,白石光也
    • Organizer
      第155回日本獣医学会学術集会
    • Place of Presentation
      東京都
    • Year and Date
      2013-03-30
    • Related Report
      2013 Final Research Report
  • [Presentation] メチル水銀の細胞毒性発現におけるMARCKSの役割2013

    • Author(s)
      白石光也,半谷誠,佐々木眞敬,宮本篤
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡市
    • Year and Date
      2013-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] メチル水銀によるMARCKSリン酸化機序と毒性発現との関連2012

    • Author(s)
      白石光也,半谷誠,佐々木眞敬,宮本篤
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      盛岡市
    • Year and Date
      2012-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Alteration of MARCKS Expression and Phosphorylation Levels by Methylmercury

    • Author(s)
      Mitsuya Shiraishi, Makoto Hangai, Megumi Yamamoto, Atsuhiro Tanabe, Atsushi Miyamoto
    • Organizer
      The XIII International Congress of Toxicology
    • Place of Presentation
      Coex (Seoul), Korea
    • Related Report
      2013 Annual Research Report
  • [Presentation] SH-SY5Y細胞におけるfura-2とTPENを用いた重金属誘導細胞内カルシウム動員の解析

    • Author(s)
      大久保正人,宮本篤,白石光也
    • Organizer
      第87回日本薬理学会年会
    • Place of Presentation
      仙台国際センター(仙台市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] メチル水銀によるMARCKSリン酸化機序と毒性発現との関連.

    • Author(s)
      白石光也,半谷誠,佐々木眞敬,宮本篤.
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手大学(盛岡市)
    • Related Report
      2012 Research-status Report
  • [Presentation] ブラジキニン刺激による神経突起伸長へのMARCKSリン酸化/脱リン酸化の関与.

    • Author(s)
      田辺敦弘, 白石光也, 佐々木泰治.
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Related Report
      2012 Research-status Report
  • [Presentation] メチル水銀の細胞毒性発現におけるMARCKSの役割.

    • Author(s)
      白石光也,半谷誠,佐々木眞敬,宮本篤.
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Related Report
      2012 Research-status Report
  • [Presentation] MPP+誘導性アポトーシスに対するMARCKSの関与.

    • Author(s)
      田辺敦弘,桜井祥太,白石光也,佐々木泰治.
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 重金属による細胞内カルシウム濃度の変化とその特徴.

    • Author(s)
      大久保正人,宮本篤,白石光也.
    • Organizer
      第155回日本獣医学会学術集会
    • Place of Presentation
      東京大学駒場キャンパス(東京都)
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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