| Project/Area Number |
23780302
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
TAKESHIMA Shin-nosuke 独立行政法人理化学研究所, 分子ウイルス学特別研究ユニット, 基幹研究所研究員 (60342812)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 高活性型Tax 蛋白質 / マイクロアレイ / ウイルス蛋白質 / 牛白血病ウイルス / 転写活性化 / Tax / 高活性型Tax変異体 |
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| Research Abstract |
In this study, we try to develop the new high-sensitivity BLV detection system using high-activity transactivation Tax protein. We construct High-Tax expression vector, and introduced to HeLa cells, and confirmed that the functions such as localization, G1 arrest, apoptosis inducing, did not changed for wild type and high-tax. Additionally, we performed cDNA microarray analysis and determined that Tax protein controlled cell cycle, transactivation, cell proliferation and immunity.
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