| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Research Abstract |
We examined the expression of tight junction and adherens junction protein in duodenal mucosa sample of dogs with inflammatory bowel disease (IBD). The expression of TJ proteins (claudin-1, -2, -3, -4, -5, -7 and -8), and AJ proteins (E-cadherin and beta-catenin) were determined by means of immunoblotting. As a result, expression of E-cadherin protein was significantly lower in duodenal mucosa samples of dogs with IBD than it was in samples obtained from healthy control dogs. We speculated that T helper cells have important roles in change in expression of E-cadherin in duodenal mucosa of IBD dogs. Thus, we evaluated the gene expression of cytokines of T cell subsets (IL-17, IFN-gamma, IL-4, and IL-10) in duodenal mucosa from dogs with IBD. As a result, there was no significant difference in each cytokine mRNA transcription level between groups. Thus, there is no clear evidence for the involvement of distinct T helper cytokine in the pathogenesis of canine IBD.
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