Study on the pathogenesis of canine inflammatory bowel disease: investigation of the intestinal mucosal barrier function and intestinal mucosal cytokine expression.
| Project/Area Number |
23780315
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Clinical veterinary science
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| Research Institution | Hokkaido University |
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Principal Investigator |
OHTA Hiroshi 北海道大学, (連合)獣医学研究科, 助教 (50431333)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 犬 / 腸炎 / サイトカイン / インターロイキン－１７ / 粘膜バリア / インターロイキンー１７ / クローディン / カドヘリン / Ｔ細胞 / マクロファージ |
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| Research Abstract |
We examined the expression of tight junction and adherens junction protein in duodenal mucosa sample of dogs with inflammatory bowel disease (IBD). The expression of TJ proteins (claudin-1, -2, -3, -4, -5, -7 and -8), and AJ proteins (E-cadherin and beta-catenin) were determined by means of immunoblotting. As a result, expression of E-cadherin protein was significantly lower in duodenal mucosa samples of dogs with IBD than it was in samples obtained from healthy control dogs. We speculated that T helper cells have important roles in change in expression of E-cadherin in duodenal mucosa of IBD dogs. Thus, we evaluated the gene expression of cytokines of T cell subsets (IL-17, IFN-gamma, IL-4, and IL-10) in duodenal mucosa from dogs with IBD. As a result, there was no significant difference in each cytokine mRNA transcription level between groups. Thus, there is no clear evidence for the involvement of distinct T helper cytokine in the pathogenesis of canine IBD.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] CD4(+) T Cell Cytokine Gene and Protein Expression in Duodenal Mucosa of Dogs with Inflammatory Bowel Disease2014
Author(s)
Ohta H, Takada K, Sunden Y, Tamura Y, Osuga T, Lim SY, Murakami M, Sasaki N, Wickramasekara Rajapakshage BK, Nakamura K, Yamasaki M, Takiguchi M
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Journal Title
J Vet Med Sci
Volume: 76(3)
Pages: 409-414
NAID
Related Report
Peer Reviewed
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[Journal Article] Markedly increased expression of interleukin-8 in the colorectal mucosa of inflammatory colorectal polyps in miniature dachshunds.2013
Author(s)
Tamura Y, Ohta H, Torisu S, Yuki M, Yokoyama N, Murakami M, Lim SY, Osuga T, Morishita K, Nakamura K, Yamasaki M, Takiguchi M
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Journal Title
Veterinary Immunology and Immunopathology
Volume: 156
Issue: 1-2
Pages: 32-42
DOI
Related Report
Peer Reviewed
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[Journal Article] Expression of CD4+ T cell cytokine genes in the colorectal mucosa of inflammatory colorectal polyps in miniature dachshunds.2013
Author(s)
Ohta H, Takada K, Torisu S, Yuki M, Tamura Y, Yokoyama N, Osuga T, Lim SY, Murakami M, Sasaki N, Nakamura K, Yamasaki M, Takiguchi M
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Journal Title
Veterinary Immunology and Immunopathology
Volume: 155
Issue: 4
Pages: 259-263
DOI
Related Report
Peer Reviewed
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