Study for development of a novel therapy for canine atopic dermatitis focused on signaling proteins

• Project/Area Number: 23780321
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Clinical veterinary science
• Research Institution: Gifu University
• Principal Investigator: SHIBATA Sanae 岐阜大学, 応用生物科学部, 准教授 (20588917)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: canine / keratinocytes / HB-EGF / 犬アトピー性皮膚炎 / ケラチノサイト / イヌアトピー性皮膚炎

Outline of Final Research Achievements

We focused on EGF family in order to develop a novel therapy for canine atopic dermatitis. In this study, we studied about HB-EGF which mainly expresses in epidermis. We succeeded partial cloning of canine HB-EGF mRNA. In addition, the HB-EGF expression in canine keratinocytes cell line didn't change after stimulation with inflammatory cytokine.