| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
Plant cell wall polysaccharides are useful in our daily life as a source of pulp and dietary fiber. The plant cell wall polysaccharides are also considered as important source of biofuel such as bioethanol. However, the mechanism of plant cell wall biosyntehsis has been unknown. In this project, we studied UDP-arabinopyranose mutase (UAM) and UDP-galactopyranose mutase (UGM) that are the enzymes involved in the cell wall biosynthesis in order to clear the mechanism of plant cell wall biosynthesis. Japan has a lot of species of algae which possess an ability of CO2 fixation higher than land plants do and become a source of bioenergy. Thus, we focused on algae cell wall, and used Chlamydomonas reinhardtii as a smple in this project. In order to clarify the function of these enzymes in the Chlamydomonas cell, we analyzed the characterization of Chlamydomonas UAM and detected Chlamydomonas UGM activity. We also analyzed the UAM and UGM gene expression on cell cycle.
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