Imaging of membrane dynamics in specialized differentiated cells by multiphoton super-resolution microscopy
Project/Area Number |
23790039
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Physical pharmacy
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Research Institution | Hokkaido University |
Principal Investigator |
HIBI Terumasa 北海道大学, 電子科学研究所, 助教 (50554292)
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 破骨細胞 / 脂肪細胞 / 小胞輸送 / 蛍光タンパク質 / 多光子顕微鏡 / イメージング / 膜動態 / 膜融合 |
Research Abstract |
In human body, homeostasis is maintained by functions of various specialized differentiated cells. Most of functions are due to vesicular trafficking and membrane fusion. In this work, vesicular trafficking in osteoclasts and adipocytes was analyzed with multiphoton fluorescent 4D microscopy. Additionally, combination of multiphoton microscopy with our previously developed liquid crystal devices realized both high lateral resolution and deep depth-of-field.
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Report
(3 results)
Research Products
(59 results)
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[Presentation] IMPROVEMENT OF THE SPATIAL RESOLUTION IN TWO-PHOTON MICROSCOPY WITH A VECTOR BEAM GENERATED BY LIQUID CRYSTAL DEVICES2012
Author(s)
Sari Ipponjima, Terumasa Hibi, Yuichi Kozawa, Hibiki Horanai, Aya Sato, Makoto Kurihara, Nobuyuki Hashimoto, Hiroyuki Yokoyama, Shunichi Sato, Tomomi Nemoto
Organizer
Focus on Microscopy 2012
Place of Presentation
Suntec Singapore International Convention & Exhibition centre(Singapore, Singapore)
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[Presentation] Improvement of spatial resolution in laser scanning microscopy with higher-order radially polarized beam generated by liquid crystal device2011
Author(s)
Sari Ipponjima, Terumasa Hibi, Yuichi Kozawa, Hibiki Horanai, Aya Sato, Makoto Kurihara, Nobuyuki Hashimoto, Hiroyuki Yokoyama, Shunichi Sato, Tomomi Nemoto
Organizer
12th RIES-Hokudai International Symposium
Place of Presentation
Chateraise Gateaux Kingdom Sapporo (Sapporo, Japan)
Related Report
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