Structural elucidation of the sequential signal transduction mechanism in two-component system
| Project/Area Number |
23790042
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
UEDA Takumi 東京大学, 大学院・薬学系研究科, 助教 (20451859)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 構造生物学 / NMR / 膜蛋白質 / 転移交差飽和法 / 過渡的複合体 / ニ成分制御系 / マルチドメイン蛋白質 / 過渡的相互作用 / 膜タンパク質 / タンパク質―タンパク質相互作用 / 交差飽和法 / Protein Trans-Splicing / Segmental labeling |
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| Research Abstract |
Our NMR study based on cross-saturation method revealed that the CheA-CheY complex, which regulates bacterial chemotaxis, exists in equilibrium between catalytic and non-catalytic states, in which their catalytic sites are close to and away from each other, respectively. This binding mode allows CheA to perform two signal transduction reactions with one catalytic site.
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Report
(3 results)
Research Products
(15 results)
