| Project/Area Number |
23790051
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Nagasaki University |
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Principal Investigator |
SHIBATA Takayuki 長崎大学, 医歯薬学総合研究科(薬学系), 助教 (10448491)
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| Research Collaborator |
FUJITA Jun-ya
DRAGUSHA Shpend
YIN Sheng
SHIMAMURA Ryosuke
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | DPD欠損症 / ウラシル / ベンズアミドオキシム / 蛍光反応 / 臨床検査 / スクリーニング / シトシン / オロト酸 / 尿中ウラシル定量 / 核酸代謝 / ハイスループット診断 / ウラシル特異性 |
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| Outline of Final Research Achievements |
In this research, a uracil-specific fluorogenic reaction developed by our research group was applied to the quantification of urinary uracil concentration for the development of a facile and rapid diagnostic method of DPD deficiency. As a result, we have succeeded in a simultaneous analysis of urinary uracil level using multiple samples, which requires a simple addition of aqueous reagent solutions and heating for a few minutes. The result suggested that the present method would be applicable to the screening test of DPD deficiency at a clinical site. In addition, by changing the reaction conditions of the uracil-specific fluorogenic reaction, we also have discovered novel fluorescence derivatization methods specific to cytosine and orotic acid, respectively.
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