Role of a novel photoproduct found in sensory rhodopsin II in the function and its characterization

• Project/Area Number: 23790059
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Matsuyama University
• Principal Investigator: TAMOGAMI Jun 松山大学, 薬学部, 助教 (30580089)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: センサリーロドプシンII / 光受容タンパク質 / 光情報伝達 / 走光性 / 分子間相互作用

Research Abstract

Sensory rhodopsin II (SRII) is a photophobic receptor protein in halobacteria. In this study, we attempted to characterize a long-lived photoproduct (Mlike intermediate) that was formed in SRII from Halobacterium salinarum under continuous illumination and reveal the physiological role of it. Through the experiment using hydroxylamine, a water-soluble reagent, the hydrophilic environmental change in the protein interior at Mlike state was observed. In addition, the effects of the mutation of two amino acid residues (Asp103 and Ser201) located in the vicinity of the retinal on the phototaxis were investigated.

Research Products

• [Journal Article] A study of the interaction of drugs with liposomes with isothermal titration calorimetry (2013)
  • Author(s): Osanai, H., Ikehara, T., Miyauchi, S., Shimono, K., Tamogami, J., Nara, T.&Kamo, N
  • Journal Title: J. Biophys. Chem Volume: 4 Pages: 11-21
  • Peer Reviewed
• [Journal Article] Thermodynamic parameters of anion binding to halorhodopsin from Natronomonas pharaonis by isothermal titration calorimetry (2013)
  • Author(s): Hayashi, S., Tamogami, J., Kikukawa, T., Okamoto, H., Shimono, K., Miyauchi, S., Demura, M., Nara, T. & Kamo, N
  • Journal Title: Biophys. Chem Volume: 172 Pages: 61-67
  • Peer Reviewed
• [Journal Article] Thermodynamic parameters of anion binding to halorhodopsin from Natronomonas pharaonis by isothermal titration calorimetry (2013)
  • Author(s): Hayashi, S., J. Tamogami, T. Kikukawa, H. Okamoto, K. Shimono, S. Miyauchi, M. Demura, T. Nara, N. Kamo
  • Journal Title: Biophys. Chem. Volume: 172 Pages: 61-67
  • Peer Reviewed
• [Journal Article] A study of the interaction of drugs with liposomes with isothermal titration calorimetry (2013)
  • Author(s): Osanai, H., T. Ikehara, S. Miyauchi, K. Shimono, J. Tamogami, T. Nara, N. Kamo
  • Journal Title: Journal of Biophysical Chemistry Volume: 4 Pages: 11-21
  • Peer Reviewed
• [Journal Article] Photoinduced proton release in proteorhodopsin at low pH : The possibility of a decrease in the pK_a of Asp227 (2012)
  • Author(s): Tamogami, J., Kikukawa, T., Nara, T., Shimono, K., Demura, M. & Kamo, N
  • Journal Title: Biochemistry Volume: 51 Pages: 9290-9301
  • Peer Reviewed
• [Journal Article] Photo-induced bleaching of sensory rhodopsin II (phoborhodopsin) from Halobacterium salinarum by hydroxylamine : Identification of responsible intermediates (2012)
  • Author(s): Tamogami, J., Kikukawa, T., Ikeda, Y., Demura, M., Nara, T. & Kamo, N
  • Journal Title: J. Photochem. Photobiol. B. Volume: 106 Pages: 87-94
  • Peer Reviewed
• [Journal Article] Photoinduced proton release in proteorhodopsin at low pH: The possibility of a decrease in the pKa of Asp227 (2012)
  • Author(s): Tamogami, J., T. Kikukawa, T. Nara, K. Shimono, M. Demura, N. Kamo
  • Journal Title: Biochemistry Volume: 51 Pages: 9290-9301
  • Peer Reviewed
• [Journal Article] Photo-induced bleaching of sensory rhodopsin II (phoborhodopsin) from Halobacterium salinarum by hydroxylamine: Identification of responsible intermediates (2012)
  • Author(s): Tamogami, J., T. Kikukawa, Y. Ikeda, M. Demura, T. Nara, N. Kamo
  • Journal Title: J. Photochem. Photobiol. B. Volume: 106 Pages: 87-94
  • Peer Reviewed
• [Journal Article] 細菌の走光性を解明する (2011)
  • Author(s): 奈良敏文、田母神淳、加茂直樹
  • Journal Title: バイオメカニズム学会誌 Volume: 35 Pages: 224-232
  • NAID: 10030038822
  • Peer Reviewed
• [Journal Article] Photochemistry of Acetabularia rhodopsin II from a marine plant, Acetabularia acetabulum (2011)
  • Author(s): Kikukawa, T., Shimono, K., Tamogami, J., Miyauchi, S., Kim, S-Y., Kimura-Someya, T., Shirouzu, M., Jung, K-H., Yokoyama, S. & Kamo, N
  • Journal Title: Biochemistry Volume: 50 Pages: 8888-8898
  • Peer Reviewed
• [Journal Article] An amino acid residue (S201) in the retinal binding pocket regulates the photoreaction pathway of phoborhodopsin (2011)
  • Author(s): Dai, G., Zhang, Y., Tamogami, J., Demura, M., Kamo, N., Kandori, H. & Iwasa, T
  • Journal Title: Biochemistry Volume: 50 Pages: 7177-7183
  • Peer Reviewed
• [Journal Article] Crystal structure of the eukaryotic light-driven proton pumping rhodopsin, Acetabularia rhodopsin II from marine alga (2011)
  • Author(s): Wada, T., Shimono, K., Kikukawa, T., Hato, M., Shinya, N., Kim, S-Y., Kimura-Someya, T., Shirouzu, M., Tamogami, J., Miyauchi, S., Jung, K-H., Kamo, N. and Yokoyama, S
  • Journal Title: J. Mol. Biol Volume: 411 Pages: 986-998
  • Peer Reviewed
• [Journal Article] Crystal structure of the eukaryotic light-driven proton pumping rhodopsin, Acetabularia rhodopsin II from marine alga (2011)
  • Author(s): Wada, T., K. Shimono, T. Kikukawa, M. Hato, N. Shinya, S-Y. Kim, T. Kimura-Someya, M. Shirouzu, J. Tamogami, S. Miyauchi, K-H. Jung, N. Kamo, S. Yokoyama
  • Journal Title: J. Mol. Biol. Volume: 411 Pages: 986-998
  • Peer Reviewed
• [Presentation] 海藻由来のロドプシン様タンパク質アセタブラリアロドプシンIの光化学的性質の解析 (2014)
  • Author(s): 田母神淳
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本、日本
  • Year and Date: 2014-03-29
• [Presentation] Photochemical reaction in Acetabularia rhodopsin I (2013)
  • Author(s): 田母神淳
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都、日本
  • Year and Date: 2013-10-29
• [Presentation] プロテオロドプシンの低pH条件下で起こるプロトン放出 (2013)
  • Author(s): 田母神淳
  • Organizer: 第5回日本生物物理学会中国四国支部大会
  • Place of Presentation: 香川、日本
  • Year and Date: 2013-05-26
• [Presentation] Natronomonas pharaonis由来のセンサリーロドプシンIIのAsp193近傍への塩化物イオンの結合 (2013)
  • Author(s): 田母神淳
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜、日本
  • Year and Date: 2013-03-29
• [Presentation] The proton release in proteorhodopsin at low pH : Decrease in pKa of Asp227 in the excited state (2012)
  • Author(s): Tamogami, J
  • Organizer: 15^<th> International Conference on Retinal Proteins (ICRP)
  • Place of Presentation: Ascona, Switzerland
  • Year and Date: 2012-10-01
• [Presentation] The role of chloride in the photo-induced proton transfer in sensory rhodopsin II from Natronomonas pharaonis (2012)
  • Author(s): 田母神淳
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋、日本
  • Year and Date: 2012-09-23
• [Presentation] Natronomonas pharaonis由来のセンサリーロドプシンII(NpSRII)の光誘起プロトン放出機構に及ぼす塩化物イオンの影響 (2012)
  • Author(s): 田母神淳
  • Organizer: 第4回日本生物物理学会中国四国支部大会
  • Place of Presentation: 山口、日本
  • Year and Date: 2012-06-02
• [Presentation] 海洋細菌由来のロドプシン様タンパク質プロテオロドプシンの低pH条件下における速いプロトン放出機構 (2012)
  • Author(s): 田母神淳
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌、日本
  • Year and Date: 2012-03-30
• [Presentation] The proton release in proteorhodopsin at low pH: Decrease in pKa of Asp227 in the excited state (2012)
  • Author(s): Jun Tamogami, Takashi Kikukawa, Toshifumi Nara, Kazumi Shimono, Makoto Demura, Naoki Kamo
  • Organizer: 15th International Conference on Retinal Proteins (ICRP)
  • Place of Presentation: Ascona, Switzerland
• [Presentation] 海洋細菌由来のロドプシン様タンパク質プロテオロドプシンの低pH条件下における速いプロトン放出機構 (2012)
  • Author(s): 田母神淳、菊川峰志、下野和実、奈良敏文、宗行英朗、加茂直樹
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] H. salinarum sensory rhodopsin IIのシグナル伝達：Ser201の関与の有無について (2012)
  • Author(s): 奈良敏文、松波梨佐、石川隼也、田母神淳、加茂直樹
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] The fast proton release of proteorhodopsin at low pH (2011)
  • Author(s): 田母神淳
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫、日本
  • Year and Date: 2011-09-17
• [Presentation] センサリーロドプシンIIのヒドロキシルアミンによるブリーチング反応 (2011)
  • Author(s): 田母神淳
  • Organizer: 第3回日本生物物理学会中国四国支部大会
  • Place of Presentation: 広島、日本
  • Year and Date: 2011-05-14
• [Presentation] センサリーロドプシンIIのヒドロキシルアミンによるブリーチング反応 (2011)
  • Author(s): 田母神淳、菊川峰志、池田陽一、出村誠、奈良敏文、加茂直樹
  • Organizer: 第3回 日本生物物理学会中国四国支部大会
  • Place of Presentation: 広島
• [Presentation] 負の走光性レセプターpPR（NpSRII）と大腸菌走化性レセプターTsrの機能的相互作用 (2011)
  • Author(s): 奈良敏文、田母神淳、加茂直樹
  • Organizer: 第3回 日本生物物理学会中国四国支部大会
  • Place of Presentation: 広島
• [Presentation] 大腸菌走化性受容体Tarと基質との相互作用の等温滴定型熱量計（ITC）による測定： 比較的結合が弱い場合の解析 (2011)
  • Author(s): 角井碧、宮内正二、下野和実、奈良敏文、田母神淳、加茂直樹
  • Organizer: 第3回 日本生物物理学会中国四国支部大会
  • Place of Presentation: 広島
• [Presentation] キサントロドプシンの光誘起プロトン移動の解析 (2011)
  • Author(s): 菊川峰志、佐藤由美、田母神淳、下野和実、神谷昌克、相沢智康、河野敬一、出村誠、加茂直樹
  • Organizer: 第11回 日本蛋白質科学会年会
  • Place of Presentation: 大阪
• [Presentation] The fast proton release of proteorhodopsin at low pH (2011)
  • Author(s): Jun Tamogami, Takashi Kikukawa, Kazumi Shimono, Toshifumi Nara, Eiro Muneyuki, Naoki Kamo
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫
• [Presentation] プロテオロドプシンの低pH条件下で起こるプロトン放出
  • Author(s): 田母神淳、菊川峰志、奈良敏文、下野和実、出村誠、加茂直樹
  • Organizer: 第5回 日本生物物理学会中国四国支部大会
  • Place of Presentation: 香川
• [Presentation] 大腸菌多剤排出系EmrE の結晶構造に関する考察：細胞内外の向きを決める
  • Author(s): 奈良敏文、根来信敬、田母神淳、加茂直樹
  • Organizer: 第5回 日本生物物理学会中国四国支部大会
  • Place of Presentation: 香川
• [Presentation] Photochemical reaction in Acetabularia rhodopsin I
  • Author(s): Jun Tamogami, Takashi Kikukawa, Kazumi Shimono, Tomomi Kimura-Someya, Mikako Shirouzu, Shigeyuki Yokoyama, Naoki Kamo
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都
• [Presentation] 海藻由来のロドプシン様タンパク質アセタブラリアロドプシンIの光化学的性質の解析
  • Author(s): 田母神淳、菊川峰志、下野和実、染谷友美、白水美香子、横山茂之、加茂直樹
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
• [Presentation] Natronomonas pharaonis由来のセンサリーロドプシンII（NpSRII）の光誘起プロトン放出機構に及ぼす塩化物イオンの影響
  • Author(s): 田母神淳、岩野亮宗、松山淳、菊川峰志、出村誠、下野和実、奈良敏文、加茂直樹
  • Organizer: 第4回 日本生物物理学会中国四国支部大会
  • Place of Presentation: 山口
• [Presentation] The role of chloride in the photo-induced proton transfer in sensory rhodopsin II from Natronomonas pharaonis
  • Author(s): Jun Tamogami, Katsunori Iwano, Atsushi Matsuyama, Takashi Kikukawa, Makoto Demura, Kazumi Shimono, Toshifumi Nara, Naoki Kamo
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋
• [Presentation] Natronomonas pharaonis由来のセンサリーロドプシンIIのAsp193近傍への塩化物イオンの結合
  • Author(s): 田母神淳、岩野亮宗、松山淳、菊川峰志、出村誠、下野和実、奈良敏文、加茂直樹
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
• [Presentation] 7回膜貫通型受容体の大腸菌における発現の改善
  • Author(s): 奈良敏文、水本あきな、田母神淳、加茂直樹
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
• [Book] "Photo-induced proton transfers of microbial rhodopsins" Molecular Photochemistry- Various aspects (chapter 5) (2012)
  • Author(s): Kikukawa, T., Tamogami, J., Shimono, K., Demura, M., Nara, T. & Kamo, N
  • Publisher: InTech
• [Book] Molecular Photochemistry- Various aspects (chapter 5) (2012)
  • Author(s): Kikukawa, T., J. Tamogami, K. Shimono, M. Demura, T. Nara, N. Kamo
  • Publisher: InTech
• [Remarks]
  • URL: http://ghp01.matsuyama-u.ac.jp/~yakugaku/laboratory/labo_biophysical-chemistry.html