Structure determination and elucidation of transport mechanism of mitochondrial inner membrane transporter

• Project/Area Number: 23790064
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: TAKEUCHI Koh 独立行政法人産業技術総合研究所, 創薬分子プロファイリング研究センター, 主任研究員 (20581284)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 構造生物学 / 核磁気共鳴 / 膜タンパク質 / タンパク質発現 / 立体構造解析

Research Abstract

To contribute the understanding and cure of the human diseases associated with mitochondrial membrane transporters (MTPs), we developed prokaryotic expression systems for 13 types of MTPs for structure determination and elucidation of their transport mech

Research Products

• [Journal Article] A homogeneous, high-throughput assay for phosphatidylinositol 5-phosphate 4-kinase with a novel, rapid substrate preparation (2013)
  • Author(s): Davis MI, Sasaki AT, Shen M, Emerling BM, Thorne N, Michael S, Pragani R, Boxer M, Sumita K, Takeuchi K, Auld DS, Li Z, Cantley LC, Simenov A
  • Journal Title: PLoS One. Volume: 8 Issue: 1 Pages: e54127-e54127
  • DOI: 10.1371/journal.pone.0054127
  • Peer Reviewed
• [Journal Article] Functional dynamics of proteins revealed by solution NMR (2012)
  • Author(s): Osawa M, Takeuchi K, Ueda T, Nishida N, Shimada I.
  • Journal Title: Curr Opin Struct Biol Volume: 22 Issue: 5 Pages: 660-669
  • DOI: 10.1016/j.sbi.2012.08.007
  • Peer Reviewed
• [Journal Article] Structural basis for the Golgi association by the pleckstrin homology domain of the ceramide trafficking protein (CERT) (2012)
  • Author(s): Sugiki T, Takeuchi K, Yamaji T, Takano T, Tokunaga Y, Kumagai K, Hanada K, Takahashi H, and Shimada I
  • Journal Title: J Biol Chem Volume: 287 Issue: 40 Pages: 33706-33718
  • DOI: 10.1074/jbc.m112.367730
  • Peer Reviewed
• [Journal Article] Functional equilibrium of the KcsA structure revealed by NMR (2012)
  • Author(s): Imai S, Osawa M, Mita K, Toyonaga S, Machiyama A, Ueda T, Takeuchi K, Oiki S, Shimada I.
  • Journal Title: J Biol Chem Volume: 287 Issue: 47 Pages: 39634-39641
  • DOI: 10.1074/jbc.m112.401265
  • Peer Reviewed
• [Journal Article] Low g-nuclei detection experiments for bimolecular NMR (2012)
  • Author(s): Takeuchi K, Gal M, Shimada I, Wagner G
  • Journal Title: Recent Developments in Biomolecular NMR Volume: 印刷中
• [Journal Article] Speeding up direct (15) N detection : hCaN 2D NMR experiment (2011)
  • Author(s): Gal M, Edmonds KA, Milbradt AG, Takeuchi K, Wagner G
  • Journal Title: J Biomol NMR Volume: 51 Issue: 4 Pages: 497-504
  • DOI: 10.1007/s10858-011-9580-7
  • Peer Reviewed
• [Journal Article] Structure of the VP16 transactivator target in the Mediator (2011)
  • Author(s): Milbradt AG, Kulkarni M, Yi T, Takeuchi K, Sun ZY, Luna RE, Selenko P, Naar AM, Wagner G
  • Journal Title: Nat Struct Mol Biol Volume: 18 Issue: 4 Pages: 410-415
  • DOI: 10.1038/nsmb.1999
  • Peer Reviewed
• [Journal Article] Protein-ligand docking guided by ligand pharmacophore-mapping experiment by NMR (2011)
  • Author(s): Fukunishi Y, Mizukoshi Y, Takeuchi K, Shimada I, Takahashi H, Nakamura H.
  • Journal Title: J Mol Graph Model Volume: 31 Pages: 20-27
  • DOI: 10.1016/j.jmgm.2011.08.002
  • Peer Reviewed
• [Presentation] 多剤耐性転写制御因子の揺らぎによる薬剤認識機構 (2012)
  • Author(s): 竹内 恒、嶋田 一夫
  • Organizer: 新学術領域「揺らぎと生体機構」「水和と ATP」合同公開シンポジウム「ゆらぎと水-生命のエネルギーと機能の分子機構を探る」
  • Place of Presentation: 大阪ガーデンパレス(大阪府)
  • Year and Date: 2012-09-14
• [Presentation] NMR approach to study and interfere with blood coagulation responses (2012)
  • Author(s): 竹内 恒
  • Organizer: 難治性疾患統合創薬プロジェクト講演会
  • Place of Presentation: 立命館大学(滋賀県)
  • Year and Date: 2012-01-30
• [Presentation] 多剤耐性転写制御因子の揺らぎによる薬剤認識機構 (2012)
  • Author(s): 竹内 恒、嶋田 一夫
  • Organizer: 新学術領域「揺らぎと生体機構」「水和とATP」合同公開シンポジウム「ゆらぎと水－生命のエネルギーと機能の分子機構を探る」
  • Place of Presentation: 大阪府大阪市・大阪ガーデンパレス
• [Presentation] 酵母K.Lactis発現系を用いた高分子量蛋白質の重水素化およびメチル選択標識 (2012)
  • Author(s): 宮沢-大浪 真由美,竹内 恒, 杉木 俊彦, 高野 等覚, 嶋田 一夫, 高橋 栄夫
  • Organizer: 第51回NMR討論会
  • Place of Presentation: 愛知県名古屋市・ウインクあいち
• [Presentation] NMR Analysis of Functional Fluctuation in Large Molecular Weight Protein (2012)
  • Author(s): 竹内 恒、徳永 裕二、高橋 栄夫、嶋田 一夫
  • Organizer: 新学術領域「揺らぎが機能を決める生命分子の科学」国際シンポジウム(招待講演)
  • Place of Presentation: 奈良県・東大寺
• [Presentation] NMR approach to study and interfere with blood coagulation responses (2012)
  • Author(s): 竹内 恒
  • Organizer: 難治性疾患統合創薬プロジェクト講演会(招待講演)
  • Place of Presentation: 滋賀県・立命館大学
• [Presentation] HNCA-TOCSY-CANH experiments with alternate 13C-12C labeling: a set of 3D experiment with unique supra-sequential information for mainchain resonance assignment (2011)
  • Author(s): Koh Takeuchi, Maayan Gal, Hideo Takahashi, Gerhard Wagner, and Ichio Shimada
  • Organizer: ISNMR2011
  • Place of Presentation: 大桟橋ホール(神奈川県)
• [Presentation] T 細胞レセプターはダイナミックかつ協同的な 4 次元構造変化により活性化する-NMR および光ピンセット法による解析- (2011)
  • Author(s): 竹内 恒、嶋田 一夫
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 姫路工大(兵庫県)
• [Presentation] T細胞活性化のしくみTCRはナノマシーンか？-NMRおよび光ピンセット法による解析- (2011)
  • Author(s): 竹内 恒、嶋田 一夫
  • Organizer: 第６回構造生物学に関する先端技術講演会(招待講演)
  • Place of Presentation: 福岡県・九州大学
• [Presentation] T細胞レセプターはダイナミックかつ協同的な4次元構造変化により活性化する－NMRおよび光ピンセット法による解析－ (2011)
  • Author(s): 竹内 恒、嶋田 一夫
  • Organizer: 第49回日本生物物理学会年会(招待講演)
  • Place of Presentation: 兵庫県・姫路工大
• [Presentation] HNCA-TOCSY-CANH experiments with alternate 13C-12C labeling: a set of 3D experiment with unique supra-sequential information for mainchain resonance assignment (2011)
  • Author(s): Koh Takeuchi1, Maayan Gal, Hideo Takahashi, Gerhard Wagner, and Ichio Shimada
  • Organizer: ISNMR2011
  • Place of Presentation: 神奈川県・大桟橋ホール
• [Book] Royal Society of Chemistry,Recent Developments in Biomolecular NMR (2012)
  • Author(s): Takeuchi K, Gal M, Shimada I, and Wagner G 他
  • Total Pages: 364
• [Remarks]
  • URL: http://unit.aist.go.jp/birc2/
• [Remarks] http://unit.aist.go.jp/birc2/