Establishment of the analytical method of phosphoinositide by using tandem mass spectrometry.

• Project/Area Number: 23790074
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Akita University
• Principal Investigator: NAKANISHI Hiroki 秋田大学, 生体情報研究センター, 助教 (10466740)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: リピドミクス / ホスホイノシタイド / 高感度分析法 / 質量分析法 / 高感度分析 / 質量分析

Research Abstract

We developed a sensitive and robust method to determine PIPs with different fatty acid compositions by using tandem mass spectrometry. This established analytical method was applicable to various biological samples such as cells and mouse tissues. In this research, we found that fatty acid compositions of PIPs had diversity.

Research Products

• [Journal Article] 酸性リゾリン脂質を量る (2013)
  • Author(s): 中西広樹
  • Journal Title: 実験医学別冊 Pages: 118-123
• [Journal Article] The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza (2013)
  • Author(s): Morita M, Kuba K, Ichikawa A, Nakayama M, Katahira J, Iwamoto R, Watanebe T, Sakabe S, Daidoji T, Nakamura S, Kadowaki A, Ohto T, Nakanishi H, Taguchi R, Nakaya T, Murakami M, Yoneda Y, Arai H, Kawaoka Y, Penninger JM, Arita M, Imai Y.
  • Journal Title: Cell Volume: 153 Issue: 1 Pages: 112-125
  • DOI: 10.1016/j.cell.2013.02.027
  • Peer Reviewed
• [Journal Article] Global metabolomic analysis of heart tissue in a hamster model for dilated cardiomyopathy. (2013)
  • Author(s): Maekawa K, Hirayama A, Iwata Y, Tajima Y, Nishimaki-Mogami T, Sugawara S, Ueno N, Abe H, Ishikawa M, Murayama M, Matsuzawa Y, Nakanishi H, Ikeda K, Arita M, Taguchi R, Minamino N, Wakabayashi S, Soga T, Saito Y.
  • Journal Title: J Mol Cell Cardiol Volume: 59 Pages: 76-85
  • DOI: 10.1016/j.yjmcc.2013.02.008
  • Peer Reviewed
• [Journal Article] Novel regulation pf cardiac metabolism and homeostasis by the adrenomedullin- receptor activity-modifying protein 2 system (2013)
  • Author(s): Takahiro Yoshizawa, Takayuki Sakurai, Akiko Kamiyoshi, Yuka Ichikawa-Shindo, Hisaka Kawate, Yasuhiro Iesato, Teruhide Koyama, Ryuichi Uetake, Lei Yang, Akihiro Yamauchi, Megumu Tanaka, Yuichi Toriyama, Kyoko Igarashi, Tsutomu Nakada, Toshihide Kashihara, Mitsuhiko Yamada, Hayato Kawakami, Hiroki Nakanishi, Ryo Taguchi, Tsuyoshi Nakanishi, Hiroshi Akazawa, Takayuki Shindo
  • Journal Title: Hypertension Volume: 61 Issue: 2 Pages: 341-351
  • DOI: 10.1161/hypertensionaha.111.00647
  • Peer Reviewed
• [Journal Article] Identification and structure determination of a novel anti-inflammatory mediator resolvin E3 : 17, 18-dihydroxy-eicosapentaenoic acid. (2012)
  • Author(s): Isobe Y, * Arita M, Matsueda S, Iwamoto R, Fujihara T, Nakanishi H, Taguchi R, Masuda K, Sasaki K, Urabe D, Inoue M, Arai H
  • Journal Title: J. Biol. Chem Volume: 287 Issue: 13 Pages: 10525-10534
  • DOI: 10.1074/jbc.m112.340612
  • Peer Reviewed
• [Journal Article] LYCAT, a homologue of C. elegans acl-8, acl-9, and acl-10, determines the fatty acid composition of phosphatidylinositol in mice (2012)
  • Author(s): Rieko Imae, Takao Inoue, Yasuko Nakasaki, Yasunori Uchida, Yohsuke Ohba, Nozomu Kono, Hiroki Nakanishi, Takehiko Sasaki, Shohei Mitani and Hiroyuki Arai
  • Journal Title: Journal of Lipid Research Volume: 53 Issue: 3 Pages: 335-347
  • DOI: 10.1194/jlr.m018655
  • Peer Reviewed
• [Journal Article] 酸性リン脂質の高感度分析法とその臨床的有意性 (2011)
  • Author(s): 中西広樹
  • Journal Title: JSB MS Letters・日本医用マススペクトル学会誌 Volume: 36 Pages: 24-30
• [Journal Article] p125/Sec23-interacting protein (Sec23ip) is required for spermiogenesis (2011)
  • Author(s): Arimitsu Nagiza
  • Journal Title: FEBS Lett. Volume: 585 Issue: 14 Pages: 2171-2176
  • DOI: 10.1016/j.febslet.2011.05.050
  • Peer Reviewed
• [Journal Article] Hair follicular expression and function of group X secreted phospholipase A_2 in mouse skin (2011)
  • Author(s): Yamamoto, K., Taketomi, T., Isogai, Y., Miki, Y., Sato, H., Masuda, S., Nishito, Y., Morioka, K., Ishimoto, Y., Suzuki, N., Yokoya, Y., Hanasaki, K., Ishikawa, Y., Ishii, T., Kobayashi, T., Fukami, K., Ikeda, K., Nakanishi, H., Taguchi, R., and Murakami, M.
  • Journal Title: J. Biol. Chem Volume: 286 Issue: 13 Pages: 11616-11631
  • DOI: 10.1074/jbc.m110.206714
  • Peer Reviewed
• [Journal Article] 酸性リン脂質の高感度分析法とその臨床的有意性. (2011)
  • Author(s): 中西広樹
  • Journal Title: JSB MS Letters・日本医用マススペクトル学会誌 Volume: 36 Pages: 24-30
• [Presentation] 質量分析計を用いたイノシトールリン脂質の一斉定量分析系の開発 (2012)
  • Author(s): 中西広樹
  • Organizer: 第54回日本脂質生化学会
  • Place of Presentation: 九州大学(福岡)
  • Year and Date: 2012-06-07
• [Presentation] 脂質メタボロミクスから理解する炎症性疾患 (2011)
  • Author(s): 中西広樹
  • Organizer: 第48回シグナル伝達医学グローバルCOE 学術講演会
  • Place of Presentation: 神戸大学(神戸市)
  • Year and Date: 2011-09-07
• [Presentation] Analyses of lipid localization/lipid metabolism on mouse retina using laser microdissection (2011)
  • Author(s): Hiroki Nakanishi
  • Organizer: The 30^<th> Naito Conference
  • Place of Presentation: シャトレーゼ ガトーキングダム サッポロ(札幌市)
  • Year and Date: 2011-06-29
• [Presentation] 質量分析計を用いたイノシトールリン脂質の高感度測定 (2011)
  • Author(s): 中西広樹
  • Organizer: 第10回生命科学研究会
  • Place of Presentation: ホテルメトロポリタン高崎(高崎市)
  • Year and Date: 2011-06-24
• [Presentation] 質量分析計を用いたイノシトールリン脂質の高感度測定 (2011)
  • Author(s): 中西広樹
  • Organizer: 第10回生命科学研究会
  • Place of Presentation: ホテルメトロポリタン高崎（高崎市）
• [Presentation] Analyses of lipid localization/lipid metabolism on mouse retina using laser microdissection. (2011)
  • Author(s): Hiroki Nakanishi
  • Organizer: The 30th Naito Conference
  • Place of Presentation: シャトレーゼ ガトーキングダム サッポロ（札幌市）
• [Presentation] 脂質メタボロミクスから理解する炎症性疾患 (2011)
  • Author(s): 中西広樹
  • Organizer: 第48回シグナル伝達医学グローバルCOE学術講演会(招待講演)
  • Place of Presentation: 神戸大学（神戸市）
• [Presentation] 質量分析計を用いたイノシトールリン脂質の一斉定量分析系の開発
  • Author(s): 中西広樹
  • Organizer: 第５４回日本脂質生化学会
  • Place of Presentation: 九州大学医学部百年講堂（福岡）
• [Book] Lipidomics, Technologies and Applications (2012)
  • Author(s): Taguchi R., Ikeda K., Nakanishi H
  • Publisher: Lipidomics for elucidation of Metabolic Syndrome and related Lipid Metabolic Disorder
• [Book] 試料分析講座・脂質分析 (2011)
  • Author(s): 中西広樹
  • Publisher: リン脂質およびリン脂質酸化物の分析
• [Book] 試料分析講座・脂質分析 (2011)
  • Author(s): 中西広樹
  • Publisher: MS イメージングによる脂質の局在解析
• [Book] 試料分析講座・脂質分析 (2011)
  • Author(s): 中西広樹
  • Publisher: 丸善出版株式会社
• [Remarks]