Transcriptional regulation of nuclear long noncoding RNA in mammalian cells

• Project/Area Number: 23790077
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: AKIMITSU Nobuyoshi 東京大学, アイソトープ総合センター, 准教授 (40294962)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ノンコーディング RNA / がん / 転写 / 53 / RNA / ノンコーディングＲＮＡ / 非コードＲＮＡ / がん抑制遺伝子

Research Abstract

In this study, I examined the function of MALAT1, a nuclear long noncoding RNA, in transcriptional regulation. I found that MALAT1 negatively regulates the promoter activity of p53, a well-known anti-cancer gene. This result can well-explain the relationship between MALAT1 and cancer based on gene regulation of p53.

Research Products

• [Journal Article] Psammaplin Ainhibits hepatitis C virus NS3 helicase, J.Natur. Med., in press. (2013)
  • Author(s): Salam KA., Furuta A., Noda N., Tsuneda S.Sekiguchi Y., Yamashita A., Moriishi K.,Nakakoshi M., Tsubuki M., Tani H.
  • Journal Title: TanakaJ. and Akimitsu N.,
  • Peer Reviewed
• [Journal Article] Akimitsu N, Tanaka J, Tani H, Yamashita A, Moriishi K, Nakakoshi M, Tsubuki M, Sekiguchi Y, Tsuneda S, Noda N. (2013) Cholesterol sulfate as a potential inhibitor of hepatitis C virus NS3 helicase. (2013)
  • Author(s): Furuta A, Salam KA
  • Journal Title: J. Enzyme Inhib. Med. Chem., inpress.
  • Peer Reviewed
• [Journal Article] RNA ダイナミクスから迫 る生命現象 (2013)
  • Author(s): 秋光信佳
  • Journal Title: 薬事日報、3月特集号
• [Journal Article] The RNA degradation pathway regulates the function of GAS5 a non-coding RNA in mammalian cells (2013)
  • Author(s): Tani T.Torimura M. and Akimitsu N.
  • Journal Title: PLoS ONE. Volume: 8
  • Peer Reviewed
• [Journal Article] Identification of hundreds of novel UPF1 target transcripts by direct determining whole transcriptomestability in mammalian cells (2013)
  • Author(s): Tani H., Imamachi N., Salam KA., Mizutani R., Ijiri K., Irie T., Yada T., Suzuki Y., and Akimitsu N., (
  • Journal Title: RNA Biology Volume: 9 Pages: 1370-1379
  • Peer Reviewed
• [Journal Article] Inhibition of both protease and helicase activities of hepatitis C virus NS3 by an ethyl acetate extract of marine sponge Amphimedon sp. (2012)
  • Author(s): Fujimoto Y., Salam KA., Furuta A., Matsuda Y., Fujita O., Tani H., Ikeda M., Kato N., Sakamoto N., Maekawa S., Enomoto N., de Voogd NJ., Nakakoshi M., Tsubuki M., Sekiguchi Y., Tsuneda S.Akimitsu N., Noda N., Yamashita A., Tanaka J. and Moriishi K., (
  • Journal Title: PLoS ONE Volume: 7
  • Peer Reviewed
• [Journal Article] Genome-wide determination of RNA stability reveals hundreds of short-lived non-coding transcripts in mammals (2012)
  • Author(s): Tani H., Mizutani R., Salam KA. Tano K., Ijiri K., Wakamatsu A., Isogai T., Suzuki Y. and Akimitsu N.,
  • Journal Title: Genome Res. Volume: 22 Pages: 947-956
  • Peer Reviewed
• [Journal Article] Identification of cis-and trans-acting factors involved in the localization of MALAT-1 noncoding RNA tonuclear speckles. (2012)
  • Author(s): Miyagawa R., Tano K., Mizuno R., Nakamura Y., Ijiri K., Rakwal R., Shibato J., Masuo Y., Mayeda A., Hirose T. and Akimitsu N.
  • Journal Title: RNA. Volume: 18 Pages: 738-751
  • Peer Reviewed
• [Journal Article] Identification and characterization of novel genotoxic stress-inducible nuclear long noncoding RNAs in mammalian cells. (2012)
  • Author(s): Mizutani R., Wakamatsu A., Tanaka N., Yoshida H., Tochigi N., Suzuki Y., Oonishi T., Tani H., Tano K., Ijiri K., Isogai T. and Akimitsu N.
  • Journal Title: PLoS ONE Volume: 7
  • Peer Reviewed
• [Journal Article] Inhibition of hepatitis C virusreplication and viral helicase by ethyl acetate extract of the marine feather star Alloeocomatella polycladia. (2012)
  • Author(s): Yamashita A., Salam KA., Furuta A., Matsuda Y., Fujita O., Tani H., Fujita Y., Fujimoto Y., Ikeda M., Kato N., Sakamoto N., Maekawa S., Enomoto N., Nakakoshi M., Tsubuki M., Sekiguchi Y., Tsuneda S., Akimitsu N., Noda N., Tanaka J., Moriishi K.
  • Journal Title: Marine Drugs Volume: 10 Pages: 744-761
  • Peer Reviewed
• [Journal Article] Inhibition of hepatitis C virus NS3 helicase by Manoalide. (2012)
  • Author(s): Salam KA., Furuta A., Noda N., Tsuneda S., Sekiguchi Y., Yamashita A., Moriishi K., Nakakoshi M., Tsubuki M., Tani H., Tanaka J. and Akimitsu N.
  • Journal Title: J. Nat. Prod. Volume: 75 Pages: 650-654
  • Peer Reviewed
• [Journal Article] ゲノムワイドなRNA分解測定 (2012)
  • Author(s): 秋光信佳
  • Journal Title: 細胞工学別冊「次世代シークエンサー目的別アドバンストメソッド」 Pages: 100-108
• [Journal Article] ノンコーディング RNA から理解する「がん」のメカニズム (2012)
  • Author(s): 秋光信佳
  • Journal Title: 科学フォーラム Volume: 338 Pages: 36-39
• [Journal Article] 核内構造とエピジェネティック制御をつなぐ核内長鎖ノンコーディングRNA (2012)
  • Author(s): 秋光信佳
  • Journal Title: 細胞工学 Volume: 31 Pages: 917-922
• [Journal Article] RNA の安定性を指標にして機能不明な長鎖ノンコーディング RNAの性質を定義する (2012)
  • Author(s): 谷英典、水谷玲菜、鈴木穣、秋光信佳
  • Journal Title: 細胞工学 Volume: 31 Pages: 926-927
• [Journal Article] Identification of cis- and trans-acting factors involved in the localization of MALAT-1 noncoding RNA to nuclear speckles (2012)
  • Author(s): Miyagawa, R.
  • Journal Title: RNA Volume: 18 Issue: 4 Pages: 738-751
  • DOI: 10.1261/rna.028639.111
  • Peer Reviewed
• [Presentation] 核内RNA 分解機構を介した非コードRNA機能制御 (2013)
  • Author(s): 秋光信佳
  • Organizer: 日本薬学会113年会
  • Place of Presentation: 横浜市
• [Remarks]
  • URL: http://www.ric.u-tokyo.ac.jp/akimitsu/