| Project/Area Number |
23790079
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ASAOKA Yoichi 東京医科歯科大学, 難治疾患研究所, 助教 (10436644)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 発生生物学 / Hippoシグナル伝達系 / 蛍光イメージング / 小型魚類 / YAP / モルフォリノ / 細胞張力 / 細胞間張力 / 変異体 / トランスジェニック / FRET / Fucci |
|---|
| Research Abstract |
To monitor the cell-cycle progression in the developing medaka embryos, we adapted the fluorescent ubiquitylation-based cell cycle indicator (Fucci) system. We observed that proliferating cells were concentrated in several organs, including the retina and brain during later morphogenesis. Next, we investigated whether the Hippo signaling pathway affects later morphogenesis during early zebrafish development. Knockdown of the Mst2, one of the major components of the Hippo pathway, induced morphological defects in the zebrafish eye, implying that the Hippo pathway plays a critical role inthe retinal cell proliferation and differentiation.
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