Analysis of a novel prepro-BDNF expression and function

• Project/Area Number: 23790080
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: University of Toyama
• Principal Investigator: FUKUCHI Mamoru 富山大学, 大学院・医学薬学研究部(薬学), 助教 (40432108)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: BDNF / スプライシング / 神経活動 / BDNF前駆体

Research Abstract

In this study, I investigated the mechanism of mRNA expression of brain-derived neurotrophic factor （BDNF), which is involved in expressing a variety of neuronal functions. I found that RNA splicing of BDNF exon I-IX, which is one of alternative BDNF transcripts, was enhanced by Ca2+-CaMK signal evoked by neuronal activity. Moreover, a novel prepro-BDNF protein was generated from BDNF exon I-IX mRNA. These results provide a novel insight into BDNF expression in neurons

Research Products

• [Journal Article] Deltamethrin, a type II pyrethroid insecticide, has neurotrophic effects on neurons with continuous activation of the Bdnf promoter (2012)
  • Author(s): Ihara D., Fukuchi M., Honma D., Takasaki I., Ishikawa M., Tabuchi A., and Tsuda M.
  • Journal Title: Neuropharmacology Volume: 62 Pages: 1091-1098
  • Peer Reviewed
• [Journal Article] Synthesis and biological evaluation of pyrethroid insecticide-derivatives as a chemical inducer for Bdnf mRNA expression in neurons. (2012)
  • Author(s): Matsuya Y., Ihara D., Fukuchi M.,Honma D., Itoh K., Tabuchi A., Nemoto H., and Tsuda M.
  • Journal Title: Bioorg. Med. Chem. Volume: 20 Pages: 2564-2571
  • Peer Reviewed
• [Journal Article] Synthesis and biological evaluation of pyrethroid insecticide-derivatines as a chenical inducer for Bdnf mRNA expression in neurons (2012)
  • Author(s): Matsuya, Y., Ihara, D., Fukuchi, M., Honma, D., Itoh, K., Tabuchi, A., Nemoto, H., Tsuda, M.
  • Journal Title: Bioorg.Med.Chem. Volume: 20 Issue: 8 Pages: 2564-2571
  • DOI: 10.1016/j.bmc.2012.02.048
  • Peer Reviewed
• [Journal Article] Deltamethrin, a type II pyrethroid insecticide, has neurotrophic effects on neurons with continuous activation of the Bdnf promoter (2011)
  • Author(s): Ihara, D., Fukuchi, M., Honma, D., Takasaki, I., Ishikawa, M., Tabuchi, A.Tsuda, M.
  • Journal Title: Neuropharmacol Volume: 62 Issue: 2 Pages: 1091-1098
  • DOI: 10.1016/j.neuropharm.2011.10.023
  • Peer Reviewed
• [Presentation] 生物発光イメージングによる脳由来神経栄養因子BDNF発現変化のモニタリング (2013)
  • Author(s): 福地守, 和泉宏謙, 田中亜由美, 井上蘭, 森寿, 前畑陽佑, 津田正明
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
• [Presentation] 生物発光イメージングによる脳由来神経栄養因子BDNF発現変化のモニタリング (2013)
  • Author(s): 福地 守
  • Organizer: 日本薬学会第１３３年会
  • Place of Presentation: 横浜
• [Presentation] 生物発光を利用したマウス脳内における脳由来神経栄養因子BDNF遺伝子発現変化の解析 (2012)
  • Author(s): 福地守, 和泉宏謙, 田中亜由美, 井上蘭, 森寿, 前畑陽佑, 津田正明
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] G-protein-coupled receptor-mediated activity-dependent gene expression via NMDA receptors in neurons (2012)
  • Author(s): Fukuchi M., Tabuchi A., Takasaki I., Takemori H., and Tsuda M.
  • Organizer: Neuroscience 2012 (Society for Neuroscience's 42nd annual meeting)
  • Place of Presentation: New Orleans, USA
• [Presentation] BDNF誘導性Arc遺伝子転写制御におけるプロモーター近傍領域の関与 (2012)
  • Author(s): 福地守, 中島布久美, 田渕明子, 奥野浩行, 尾藤晴彦, 津田正明
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋.
• [Presentation] G-protein-coupled receptor-mediated activity-dependent gene expression via NMDA receptors in neurons. (2012)
  • Author(s): Fukuchi, Mamoru
  • Organizer: Neuroscience 2012 (Society for Neuroscience’s 42nd annual meeting)
  • Place of Presentation: New Orleans, USA
• [Presentation] BDNF誘導性Arc遺伝子転写制御におけるプロモーター近傍領域の関与. (2012)
  • Author(s): 福地 守
  • Organizer: 第３５回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] 生物発光を利用したマウス脳内における脳由来神経栄養因子BDNF遺伝子発現変化の解析. (2012)
  • Author(s): 福地 守
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] PACAPはNMDAレセプター活性化によるカルシニューリン-TORC1-CREB経路を介してBDNF遺伝子発現を誘導する (2011)
  • Author(s): 福地守, 桑名由紀, 竹森洋, 田渕明子, 津田正明
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Involvement of the promoter proximal region in brain-derived neurotrophic factor (BDNF)-induced Arc/Arg3.1 gene transcription (2011)
  • Author(s): Fukuchi M
  • Organizer: The 6th International Conference of Neurons and Brain Diseases
  • Place of Presentation: Toyama.
• [Remarks]
  • URL: http://www.pha.u-toyama.ac.jp/bioche1/index-j.html