Photoaffinity labeling of sphingosine 1-phosphate transporters

• Project/Area Number: 23790093
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Teikyo Heisei University
• Principal Investigator: KOBAYASHI Naoki 帝京平成大学, 薬学部, 助教 (90532250)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: スフィンゴシン１リン酸 / 輸送体 / トランスポーター / 脂質メディエーター / S1P / スフィンゴシン1リン酸 / 赤血球 / スフィンゴシン / 脂質

Research Abstract

Sphingosine 1-phosphate (S1P) is a bioactive signal transmitter present in blood. Blood plasma S1P plays important roles in immune system and angiogenesis. Erythrocytes and platelets synthesize and secrete S1P. However, the S1P transporters of erythrocytes and platelets have not been identified. The S1P transporters are attractive candidates for drug target. In this study, we performed photoaffinity labeling of erythrocyte and platelet proteins with photoreactive S1P analogs. We showed that the S1P transporter, SPNS2 is selectively labeled by the photoreactive S1P analog.

Research Products

• [Journal Article] β-Lactam Selectivity of Multidrug Transporters AcrB and AcrD Resides in the Proximal Binding Pocket (2014)
  • Author(s): Kobayashi,N., Tamura,N., van Veen, HW., Murakami,S. and Yamaguchi,A.
  • Journal Title: Journal of Biological Chemistry Volume: 289 Issue: 15 Pages: 10680-10690
  • DOI: 10.1074/jbc.m114.547794
  • Peer Reviewed
• [Journal Article] Molecular and physiological functions of sphingosine 1-phosphate transporters (2014)
  • Author(s): Nishi, T., Kobayashi, N., Hisano, Y., Kawahara, A. and Yamaguchi, A
  • Journal Title: Biochimica et Biophysica Acta Volume: 1841 Issue: 5 Pages: 759-765
  • DOI: 10.1016/j.bbalip.2013.07.012
  • Peer Reviewed
• [Journal Article] Mouse SPNS2 functions as a sphingoshine 1-phosphate transporter in vascular endothelial cells (2012)
  • Author(s): Hisano, Y., Kobayashi, N., Yamaguchi, A., Nishi T.
  • Journal Title: PloS ONE Volume: 7 Issue: 6 Pages: 38941-38941
  • DOI: 10.1371/journal.pone.0038941
  • Peer Reviewed
• [Presentation] 光反応性アナログを用いたスフィンゴシン1リン酸(S1P)輸送体の探索 (2014)
  • Author(s): 小林直木、山口明人、西毅
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
  • Year and Date: 2014-03-28
• [Presentation] 光反応性アナログを用いたスフィンゴシン１リン酸（S1P）輸送体の探索 (2014)
  • Author(s): 小林直木、山口明人、西毅
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
• [Presentation] 光反応性分子を用いたスフィンゴシン1リン酸輸送体の探索 (2012)
  • Author(s): 小林直木、山口明人、西毅
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-16
• [Presentation] 光反応性分子を用いたスフィンゴシン１リン酸輸送体の探索 (2012)
  • Author(s): 小林直木、山口明人、西毅
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡（福岡県）
• [Presentation] Characterization of the ATP-dependent sphingosine 1-phosphate (S1P) transporter in rat erythrocytes (2011)
  • Author(s): Kobayashi, N., Kobayashi, N., Adachi, H., Yamaguchi, A. and Nishi, T
  • Organizer: The 30th Naito Conference on Membrane Dynamics and Lipid Biology [II]
  • Place of Presentation: Domains, Droplets and Diseases (Sapporo)
• [Presentation] Characterization of the ATP-dependent sphingosine 1-phosphate (S1P) transporter in rat erythrocytes (2011)
  • Author(s): Kobayashi, N., Kobayashi, N., Adachi, H., Yamaguchi, A. and Nishi, T.
  • Organizer: The 30th Naito Conference on Membrane Dynamics and Lipid Biology [II], Domains, Droplets and Diseases
  • Place of Presentation: CHATERAISE Gateaux Kingdom SAPPORO (Hokkaido)