A novel cholinergic enhancer and its mechanism of action

• Project/Area Number: 23790097
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Keio University
• Principal Investigator: OKUDA Takashi 慶應義塾大学, 薬学部, 講師 (00322040)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: コリン作動性神経 / アセチルコリン / コリントランスポーター / トラフィッキング / トポロジー / オリゴマー / エンドサイトーシス

Research Abstract

The high-affinity choline transporter (CHT1) is responsible for choline uptake,the rate-limiting step in acetylcholine synthesis at the cholinergic presynaptic terminals. Little is known about the regulation of the CHT1 trafficking or the structure-func

Research Products

• [Journal Article] Transmembrane topology and oligomeric structure of the high-affinity choline transporter (2012)
  • Author(s): Okuda, T., Osawa, C., Yamada, H., Hayashi, K., Nishikawa, S., Ushio, T., Kubo, Y., Satou, M., Ogawa, H., and Haga, T
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 42826-42834
  • Peer Reviewed
• [Journal Article] Osteopontin is an alpha motor neuron marker in the mouse spinal cord. (2012)
  • Author(s): Misawa, H., Hara, M., Tanabe S., Niikura, M., Moriwaki, Y., and Okuda, T
  • Journal Title: J. Neurosci. Res Volume: 90 Pages: 732-742
  • Peer Reviewed
• [Journal Article] Osteopontin is an alpha motor neuron marker in the mouse spinal cord (2012)
  • Author(s): Misawa H., et al., 他5名
  • Journal Title: J.Neurosci.Res. Volume: 90 Issue: 4 Pages: 732-742
  • DOI: 10.1002/jnr.22813
  • Peer Reviewed
• [Journal Article] Transmembrane topology and oligomeric structure of the high-affinity choline transporter. (2012)
  • Author(s): Okuda, T.
  • Journal Title: The Journal of biological chemistry Volume: 287 Issue: 51 Pages: 42826-42834
  • DOI: 10.1074/jbc.m112.405027
  • Peer Reviewed
• [Journal Article] Substrate-induced internalization of the high-affinity choline transporter (2011)
  • Author(s): Okuda, T., Konishi, A., Misawa, H., and Haga, T
  • Journal Title: J. Neurosci. Volume: 87 Pages: 3024-3032
  • Peer Reviewed
• [Journal Article] Substrate-induced internalization of the high-affinity choline transporter (2011)
  • Author(s): Okuda T., et al., 他3名
  • Journal Title: J.Neurosci. Volume: 31 Issue: 42 Pages: 14989-14997
  • DOI: 10.1523/jneurosci.2983-11.2011
  • Peer Reviewed
• [Presentation] Substrate-induced internalization A novel screen for endocytic motifs of vesicular neurotransmitter transporters (2012)
  • Author(s): 小山沙織、美濃田茜、神保美紗子、三澤日出巳、奥田隆志
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] A novel screen for endocytic motifs of vesicular neurotransmitter transporters (2012)
  • Author(s): Koyama S, Minoda A, Jimbo M, Misawa H, Okuda T
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] シナプス小胞トランスポーターの細胞内移行モチーフの探 (2011)
  • Author(s): 神保美紗子、三澤日出巳、奥田隆志
  • Organizer: 第124回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-04
• [Presentation] シナプス小胞トランスポーターの細胞内移行シグナル配列の探索 (2011)
  • Author(s): 神保美紗子、三澤日出巳、奥田隆志
  • Organizer: 第124回日本薬理学会関東部会
  • Place of Presentation: 東京大学
• [Book] コリン作動性神経機能素子の制御機構、基礎老化研究36 (3) (2012)
  • Author(s): 奥田隆志
• [Book] 高親和性コリントランスポーターの局在と機能、Clinical Neuroscience 30 (2012)
  • Author(s): 奥田隆志
• [Book] 神経変性疾患と創薬、新しい薬学事典 (2012)
  • Author(s): 奥田隆志
  • Publisher: 朝倉書店