Analysis of Microsomal prostaglandin E synthase-1 (mPGES-1) in the facial nerve regeneration.

• Project/Area Number: 23790099
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Showa University
• Principal Investigator: KAMEI Daisuke 昭和大学, 薬学部, 講師 (80515651)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 脂質 / 薬理学 / プロスタグランジン / 顔面神経麻痺 / 末梢神経 / mPGES-1 / PGE2

Research Abstract

An effective therapy is not established for aggravation of the peripheral facial nerve palsy. Microsomal prostaglandin E synthase-1 (mPGES-1) is a stimulus-inducible enzyme that functions downstream of cyclooxygenase in the prostaglandin E2 (PGE2)-biosynthetic pathway. In this study, we examined the in vivo role of mPGES-1 in the facial nerve regeneration using a mouse model of facial nerve crush injury. In vivo studies showed that contribution of PGE2 and mPGES-1 was found in the process of regeneration of facial nerve after crush injury. This study suggested that inhibition of PGE2 was useful as a new therapy for peripheral facial nerve palsy.

Research Products

• [Journal Article] Microsomal prostaglandin E synthase-1 is induced in Alzheimer's disease and its deletion mitigates Alzheimer's disease-like pathology in a mouse model (2013)
  • Author(s): Akitake Y, Nakatani Y, Kamei D, Hosokawa M, Akatsu H, Uematsu S, Akira S, Kudo I, Hara S, Takahashi M
  • Journal Title: J. Neurosci. Res Volume: 91(7) Issue: 7 Pages: 909-919
  • DOI: 10.1002/jnr.23217
  • Peer Reviewed
• [Journal Article] Advanced curriculum for clinical assessment and skill in new age pharmacist education (2013)
  • Author(s): Kiuchi Y, Masuda Y, Kamei D, Kogo M, Nakamura A.
  • Journal Title: Yakugaku Zasshi Volume: 133(2) Pages: 231-41
  • NAID: 130003361909
  • Peer Reviewed
• [Journal Article] Deletion of microsomal prostaglandin E synthase-1 protects neuronal cells from cytotoxic effects of β-amyloid peptide fragment31-35 (2012)
  • Author(s): Kuroki Y, Sasaki Y, Kamei D, Akitake Y, Takahashi M, Uematsu S, Akira S, Nakatani Y, Kudo I, Hara S
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 424(3) Issue: 3 Pages: 409-413
  • DOI: 10.1016/j.bbrc.2012.06.121
  • Peer Reviewed
• [Journal Article] Microsomal prostaglandin E synthase-1 is involved in multiple steps of colon carcinogenesis (2012)
  • Author(s): Sasaki Y, Kamei D, Ishikawa Y, Ishii T, Uematsu S, Akira S, Murakami M, Hara S
  • Journal Title: Oncogene. Volume: 31(24) Pages: 2943-52
  • Peer Reviewed
• [Journal Article] Microsomal prostaglandin E synthase-1 is involved in multiple steps of colon carcinogenesis (2012)
  • Author(s): Sasaki Y, Kamei D, Ishikawa Y, Ishii T, Uematsu S, Akira S, Murakami M, Hara S
  • Journal Title: Oncogene Volume: 31(24) Issue: 24 Pages: 2943-2952
  • DOI: 10.1038/onc.2011.472
  • Peer Reviewed
• [Journal Article] Physiological roles of group X-secreted phospholipase A2 in reproduction, gastrointestinal phospholipid digestion, and neuronal function (2011)
  • Author(s): H. Sato, Y. Isogai, S. Masuda, Y. Taketomi, Y. Miki, D. Kamei, S. Hara, T. Kobayashi, Y. Ishikawa, T. Ishii, K. Ikeda,R. Taguchi, Y. Ishimoto, N. Suzuki, Y. Yokota, K. Hanasaki, T. Suzuki-Yamamoto, K. Yamamoto, M. Murakami
  • Journal Title: J Biol Chem Volume: 286 Pages: 1632-11648
  • Peer Reviewed
• [Journal Article] Analysis of two major intracellular phospholipase A2s in mast cells reveals crucial contribution of cPLA2{alpha}, not iPLA2{beta}, to lipid mobilization in proximal mast cells and distal fibroblasts (2011)
  • Author(s): N. Ueno, Y. Taketomi, K. Yamamoto, T. Hirabayashi, D. Kamei, Y. Kita, T. Shimizu, K. Shinzawa, Y. Tsujimoto, K. Ikeda, R. Taguchi, M. Murakami
  • Journal Title: J Biol Chem Volume: 286 Pages: 37249-37263
  • Peer Reviewed
• [Journal Article] Analysis of two major intracellular phospholipase A2s in mast cells reveals crucial contribution of cPLA2alpha, not iPLA2beta, to lipid mobilization in proximal mast cells and distal fibroblasts. (2011)
  • Author(s): Ueno, N., Taketomi, Y., Yamamoto, K., Hirabayashi, T., Kamei, D., Kita, Y., Shimizu, T., Shinzawa, K., Tsujimoto, Y., Ikeda, K., Taguchi, R., Murakami, M.
  • Journal Title: J. Biol. Chem. Volume: 186 Issue: 43 Pages: 37249-37263
  • DOI: 10.1074/jbc.m111.290312
  • Peer Reviewed
• [Journal Article] Physiological roles of group X secreted phospholipase A_2 in reproduction, gastrointestinal phospholipid digestion, and neuronal function (2011)
  • Author(s): Sato, H., Isogai, Y., Masuda, S., Taketomi, Y., Miki, Y., Kamei, D., Hara, S., Kobayashi, T., Ishikawa, Y., Ishii, T., Ikeda, K., Taguchi, R., Ishimoto, Y., Suzuki, N., Yokota, Y., Hanasaki, K., Suzuki-Yamamoto, T., Yamamoto, K., and Murakami, M.
  • Journal Title: J. Biol. Chem Volume: 286 Issue: 13 Pages: 11632-11648
  • DOI: 10.1074/jbc.m110.206755
  • Peer Reviewed
• [Presentation] 末梢性顔面神経麻痺の重症化における神経炎症の影響 (2012)
  • Author(s): 竹本伊織、亀井大輔、佐久間莉乃、丸山梨詠、江崎伸一、村上信吾、小島章嗣、岩井信市、増田豊
  • Organizer: 第56回 日本薬学会 関東支部大会
  • Place of Presentation: 東京
• [Presentation] NSAIDs投与によるマウス顔面神経麻痺モデル回復過程の解析 (2012)
  • Author(s): 佐久間莉乃、亀井大輔、竹本伊織、丸山梨詠、江崎伸一、村上信吾、増田豊
  • Organizer: 日本薬学会 第132年会
  • Place of Presentation: 札幌
• [Presentation] マウス末梢性顔面神経麻痺モデルの樹立と解析 (2012)
  • Author(s): 竹本伊織、亀井大輔、佐久間莉乃、丸山梨詠、江崎伸一、村上信吾、増田豊
  • Organizer: 日本薬学会 第132年会
  • Place of Presentation: 札幌
• [Presentation] 過敏性腸症候群の治療抵抗性因子に関する検討 (2012)
  • Author(s): 秋山加菜、亀井大輔、福本滋、竹内義明、米山啓一郎、増田豊
  • Organizer: 日本薬学会 第132年会
  • Place of Presentation: 札幌
• [Presentation] 膜結合型プロスタグランジンE合成酵素をターゲットとした新たな消炎鎮痛薬の可能性 (2011)
  • Author(s): 亀井大輔
  • Organizer: 第9回 昭和ペインクリニックシンポジウム
  • Place of Presentation: 東京
• [Presentation] プロスタグランジン最終合成酵素、mPGES-1およびPGIS二重欠損マウスの性状解析 (2011)
  • Author(s): 佐々木由香、亀井大輔、松本このみ、田村夏紀, 上山修平, 原俊太郎
  • Organizer: 日本脂質生化学会 第53年会
  • Place of Presentation: 東京
• [Presentation] プロスタグランジン最終合成酵素 mPGES-1およびPGIS二重欠損マウスの性状解析 (2011)
  • Author(s): 佐々木由香、亀井大輔、松本このみ、田村夏紀, 上山修平, 原俊太郎
  • Organizer: 日本脂質生化学会 第53年会
  • Place of Presentation: 東京
• [Presentation] 膜結合型プロスタグランジンE合成酵素をターゲットとした新たな消炎鎮痛薬の可能性 (2011)
  • Author(s): 亀井大輔
  • Organizer: 第９回 昭和ペインクリニックシンポジウム(招待講演)
  • Place of Presentation: 東京