| Project/Area Number |
23790134
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Drug development chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
FUJITA Masaki 北海道大学, 創成研究機構, 特任助教 (30505251)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 天然物化学 / 生合成 / メタゲノム / 海洋天然物 / 異宿主生産 / シデロフォア |
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| Research Abstract |
More than 50 siderophore producing clones were found from the marine metagenomic library. Among them, DNA sequences of the four clones were determined. By chemical and spectroscopic methods, vibrioferrin and bisucaberin were identified as active products from the obtained clones. Especially, isolation yield of vibrioferrin was 40 times higher than that of the original producer. The other clone had a biosynthetic gene cluster whose total size was about 25 kbp which is one of the largest one found by functional metagenomic screening. These results suggested the promising potential of the functional metagenomic technique for cloning of biosynthetic genes and heterologous production of active molecules.
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