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Development of a highly efficient method for generating functional antibodies based on grafting natural ligands

Research Project

Project/Area Number 23790136
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Drug development chemistry
Research InstitutionOsaka City University

Principal Investigator

NAKANISHI Takeshi  大阪市立大学, 大学院・工学研究科, 講師 (20422074)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords抗体 / ペプチド移植 / タンパク質工学 / 受容体 / 分子認識 / リガンド
Research Abstract

In this study, to develop a method for generating functional antibodies, we grafted the peptide fragment, which was derived from the proteinous ligand, onto the variable region of a human antibody. As the results, we succeeded in preparing the peptide-gra

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (27 results)

All 2013 2012 2011 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (11 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Development of an affinity-matured humanized anti-epidermal growth factor receptor antibody for cancer immunotherapy2013

    • Author(s)
      Takeshi Nakanishi, Takamitsu Maru, Kazuhiro Tahara, Hideaki Sanada, Mitsuo Umetsu, Ryutaro Asano, and Izumi Kumagai
    • Journal Title

      Protein Eng. Des. Sel

      Volume: 26 Pages: 113-122

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of an affinity-matured humanized anti-epidermal growth factor receptor antibody for cancer immunotherapy.2013

    • Author(s)
      Takeshi Nakanishi, et al.
    • Journal Title

      Protein Engineering Design and Selection

      Volume: 26 Issue: 2 Pages: 113-122

    • DOI

      10.1093/protein/gzs088

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A high-affinity gold-binding camel antibody: Antibody engineering for one-pot functionalization of gold nanoparticles as biointerface molecules2012

    • Author(s)
      Takamitsu Hattori, Mitsuo Umetsu, Takeshi Nakanishi, Satoko Sawai, Shinsuke Kikuchi, Ryutaro Asano, and Izumi Kumagai
    • Journal Title

      Bioconjug. Chem

      Volume: 23 Pages: 1934-1944

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Construction and humanization of a functional bispecific EGFR × CD16 diabody using a refolding system2012

    • Author(s)
      Ryutaro Asano, Makoto Nakayama, Hiroko Kawaguchi, Tsuguo Kubota, Takeshi Nakanishi, Mitsuo Umetsu, Hiroki Hayashi, Yu Katayose, Michiaki Unno, Toshio Kudo, and Izumi Kumagai
    • Journal Title

      FEBS J

      Volume: 279 Pages: 223-233

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A high-affinity gold-binding camel antibody: Antibody engineering for one-pot functionalization of gold nanoparticles as biointerface molecules2012

    • Author(s)
      Takamitsu Hattori, Mitsuo Umetsu, Takeshi Nakanishi, Satoko Sawai, Shinsuke Kikuchi, Ryutaro Asano, and Izumi Kumagai
    • Journal Title

      Bioconjugate Chemistry

      Volume: 23(9) Issue: 9 Pages: 1934-1944

    • DOI

      10.1021/bc300316p

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction and humanization of a functional bispecific EGFR × CD16 diabody using a refolding system.2012

    • Author(s)
      Ryutaro Asano
    • Journal Title

      FEBS J.

      Volume: 279 Issue: 2 Pages: 223-233

    • DOI

      10.1111/j.1742-4658.2011.08417.x

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] In vitro and in vivo antitumor effects of recombinant bispecific antibodies based on humanized anti-EGFR antibody2011

    • Author(s)
      Yasuhiro Watanabe, Ryutaro Asano, Kyoko Arai, Ippei Shimomura, Hiromi Ogata, Hiroko Kawaguchi, Hiroki Hayashi, Hideo Ohtsuka, Hiroshi Yoshida, Yu Katayose, Shinichi Egawa, Takeshi Nakanishi, Mitsuo Umetsu, Hiroshi Yasui, Tadao Ishida, Kohzoh I
    • Journal Title

      Oncol. Rep

      Volume: 26 Pages: 949-955

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biomimetic engineering of modular bispecific antibodies for biomolecule immobilization2011

    • Author(s)
      Hideki Watanabe, Kengo Kanazaki, Takeshi Nakanishi, Hidenori Shiotsuka, Satoru Hatakeyama, Masaru Kaieda, Takeshi Imamura, Mitsuo Umetsu, and Izumi Kumagai
    • Journal Title

      Langmuir

      Volume: 27 Pages: 9656-9661

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cytotoxic enhancement of a bispecific diabody (Db) by format conversion to tandem single-chain variable fragment (taFv): THE CASE OF THE hEx3 DIABODY.2011

    • Author(s)
      Ryutaro Asano, Keiko Ikoma, Ippei Shimomura, Shintaro Taki, Takeshi Nakanishi, Mitsuo Umetsu, and Izumi Kumagai
    • Journal Title

      J. Biol. Chem

      Volume: 286 Pages: 1812-1818

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cytotoxic Enhancement of a Bispecific Diabody by Format Conversion to Tandem Single-chain Variable Fragment (taFv) THE CASE OF THE hEx3 DIABODY2011

    • Author(s)
      Ryutaro Asano
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 286 Issue: 3 Pages: 1812-1818

    • DOI

      10.1074/jbc.m110.172957

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] In vitro and in vivo antitumor effects of recombinant bispecific antibodies based on humanized anti-EGFR antibody2011

    • Author(s)
      Watanabe Y, Yasui H, et al
    • Journal Title

      Oncol Rep

      Volume: 26(4) Pages: 949-55

    • DOI

      10.3892/or.2011.1382

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] チロシン残基に着目した抗ムチン抗体Nd2 の抗原認識機構の解析2012

    • Author(s)
      大崎 智弘,中西 猛,星 裕孝,澤田 鉄二,木村 健二郎,内田 素行,平川 弘聖,北村 昌也
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Year and Date
      2012-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 単一ドメイン抗体可変領域を用いて設計した RNase 融合抗体の作製と機能評価2012

    • Author(s)
      工藤 光代,中西 猛,北村 昌也
    • Organizer
      第12回日本蛋白質科学会年会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2012-06-21
    • Related Report
      2012 Final Research Report
  • [Presentation] 安定性を高めたヘテロ会合ドメインの利用による抗体の高機能化2012

    • Author(s)
      大崎 智弘,藤澤 真吾,北口 将大,北村 昌也,中西 猛
    • Organizer
      第12回日本蛋白質科学会年会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2012-06-21
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] ペプチド移植による抗体様RNA 分解酵素の作製2012

    • Author(s)
      水巻 耕輔,北村 昌也,中西 猛
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-24
    • Related Report
      2012 Final Research Report
  • [Presentation] 単一ドメイン抗体可変領域を用いて設計したRNase融合抗体の作製と機能評価2012

    • Author(s)
      工藤 光代
    • Organizer
      第12回日本蛋白質科学会年会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] チロシン残基に着目した抗ムチン抗体Nd2の抗原認識機構の解析2012

    • Author(s)
      大崎 智弘
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] ペプチド移植による抗体様RNA分解酵素の作製2012

    • Author(s)
      水巻 耕輔
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Related Report
      2011 Research-status Report
  • [Presentation] がん関連抗原を標的とするリボヌクレアーゼ融合抗体の作製と評価2011

    • Author(s)
      中西 猛,工藤 光代,北村 昌也
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会館
    • Year and Date
      2011-09-22
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] EGFRを標的とした RNase 融合抗体の作製2011

    • Author(s)
      工藤 光代,北村 昌也,中西 猛
    • Organizer
      第11回日本蛋白質科学会
    • Place of Presentation
      ホテル阪急エキスポパーク
    • Year and Date
      2011-06-08
    • Related Report
      2012 Final Research Report
  • [Presentation] ヘテロ会合ペプチドの融合による低分子化抗体の多機能化2011

    • Author(s)
      中西 猛,藤澤 真吾,北村 昌也
    • Organizer
      第11回日本蛋白質科学会
    • Place of Presentation
      ホテル阪急エキスポパーク
    • Year and Date
      2011-06-08
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] EGFRを標的としたRNase融合抗体の作製2011

    • Author(s)
      工藤 光代
    • Organizer
      第11回日本蛋白質科学会
    • Place of Presentation
      ホテル阪急エキスポパーク
    • Related Report
      2011 Research-status Report
  • [Book] 次世代医薬開発に向けた抗体工学の最前線(ファージ提示系による親和性の向上)2012

    • Author(s)
      中西 猛,真田 英明,熊谷 泉
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Final Research Report
  • [Book] 次世代医薬開発に向けた抗体工学の最前線(ファージ提示系による親和性の向上)2012

    • Author(s)
      中西 猛
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.bioa.eng.osaka-cu.ac.jp/bic/

    • Related Report
      2012 Final Research Report
  • [Remarks] 北村研究室ホームページ

    • URL

      http://www.bioa.eng.osaka-cu.ac.jp/bic/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.bioa.eng.osaka-cu.ac.jp/bic/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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