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Development of methodology for site-specific RNA modification using the Reactive Oligonucleotides

Research Project

Project/Area Number 23790146
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Drug development chemistry
Research InstitutionSojo University

Principal Investigator

IMOTO Shuhei  崇城大学, 薬学部, 講師 (20447189)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
KeywordsRNA 修飾 / 架橋反応 / 核酸修飾 / 人工核酸 / RNA修飾 / クロスリンク
Research Abstract

RNA function is controlled by post-transcriptional modification. Therefore, there is increasing demand for methods of the site-specific modifications of RNA because of potential applications as biological tools and therapeutic methods. In this research, we are developing site-specific RNA modification tool using cross-linking forming oligonucleotide. We have found that the 6-vinylpurine newly designed in the current study serves well as a new nucleobase for increasing cross-link reactivity to target cytosine.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (17 results)

All 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (13 results) Remarks (1 results)

  • [Journal Article] Production of truncated protein by the crosslink formation of mRNA with 2’-OMe oligoribonucleotide containing 2-amino-6-vinylpurine2012

    • Author(s)
      Hagihara, S., Kusano, S., Lin, W.C., Chao, X.G., Hori, T., Imoto, S., Nagatsugi, F.
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 22 Issue: 12 Pages: 3870-3872

    • DOI

      10.1016/j.bmcl.2012.04.123

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fast DNA Interstrand Cross-linking Reaction by 6-Vinylpurine2012

    • Author(s)
      Imoto, S., Chikuni, T., Kansui, H, Kunieda, T. , Nagatsugi, F.
    • Journal Title

      Nucleosides Nucleotides Nucleic Acids.

      Volume: 31 Issue: 10 Pages: 752-762

    • DOI

      10.1080/15257770.2012.726756

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Imoto Induced cross-linking reactions to target genes using modified oligonucleotides2011

    • Author(s)
      F.Nagatsugi,and S.
    • Journal Title

      Organic & Biomolecular Chemistry

      Volume: 9 Issue: 8 Pages: 2579-2585

    • DOI

      10.1039/c0ob00819b

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 反応性核酸誘導体を用いたウイルス複製制御を目指した創薬研究2012

    • Author(s)
      岩本直生、草野修平、萩原伸也、佐々木要、河治久実、児玉栄一、井本修平、永次史
    • Organizer
      第12回多元物質科学研究所研究発表会
    • Place of Presentation
      東北大学、仙台
    • Year and Date
      2012-12-10
    • Related Report
      2012 Final Research Report
  • [Presentation] 架橋反応性核酸を用いた効率的蛋白質発現阻害2012

    • Author(s)
      永次史、萩原伸也、草野修平、井本修平
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学、札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] 6-ビニルプリンによるシトシンへの極めて速いDNA架橋反応2012

    • Author(s)
      井本修平、永次史、千國友子、寒水壽朗、國枝武久
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学、札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] 6-ビニルプリンによるシトシンへの極めて早い架橋反応2012

    • Author(s)
      井本 修平、千國 友子、永次 史、寒水 壽朗、國枝 武久
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report
  • [Presentation] 架橋反応性核酸を用いた効率的蛋白質発現阻害2012

    • Author(s)
      永次 史、萩原 伸也、草野 修平、井本 修平
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report
  • [Presentation] 架橋性オリゴ核酸による遺伝子発現制御2011

    • Author(s)
      萩原伸也、井本修平、堀常晃、ChaoXiao-guang、永次史
    • Organizer
      第6回日本ケミカルバイオロジー学会年会東京工業大学大岡山キャンパス
    • Related Report
      2012 Final Research Report
  • [Presentation] 新規ピリミジン誘導体を導入したオリゴ核酸の合成と架橋反応評価2011

    • Author(s)
      井本修平、永次史、寒水壽朗、國枝武久
    • Organizer
      第41回複素環化合物討論会崇城大学市民ホール
    • Place of Presentation
      熊本
    • Related Report
      2012 Final Research Report
  • [Presentation] Fumi Nagatsugi"2'-OMe RNA containing 2-amino-6-vinylpurine as a potent gene regulator2011

    • Author(s)
      Shinya Hagihara, Shuhei Imoto, Chao Xiao-guang, Tuneaki Hori, Shuhei Kusano
    • Organizer
      The 38th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      北海道大学、札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] Evaluation of the Antisense Effects Using the Reactive Oligonucleotides with High Selectivity to the Target Base2011

    • Author(s)
      Fumi Nagatsugi, Tuneaki Hori, Shuhei Kusano, Shuhei Imoto, Shinya Hagihara, Chao Xiao-Guang
    • Organizer
      8th AFMC International Medicinal Chemistry Symposium "Frontier of Medicinal Science
    • Place of Presentation
      京王プラザホテル、東京
    • Related Report
      2012 Final Research Report
  • [Presentation] グアニン6位酸素との架橋反応を目指した人工核酸塩基の開発2011

    • Author(s)
      井本修平、千國友子、永次史、寒水壽朗、國枝武久
    • Organizer
      第28回日本薬学会九州支部大会
    • Place of Presentation
      福岡大学、福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] 新規ピリミジン誘導体を導入したオリゴ核酸の合成と架橋反応評価2011

    • Author(s)
      井本 修平、永次 史、寒水 壽朗、國枝 武久
    • Organizer
      第41回複素環化学討論会
    • Place of Presentation
      熊本
    • Related Report
      2011 Research-status Report
  • [Presentation] 2'-OMe RNA containing 2-amino-6-vinylpurine as a potent gene regulator2011

    • Author(s)
      Shinya Hagihara, Shuhei Imoto, Xiau-guang Chao, Tsuneaki Hori, Shuhei Kusano, Fumi Nagatsugi
    • Organizer
      第38回国際核酸化学シンポジウム
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report
  • [Presentation] グアニン6位酸素との架橋反応を目指した人工核酸塩基の開発2011

    • Author(s)
      井本 修平、千國 友子、永次 史、寒水 壽朗、國枝 武久
    • Organizer
      第28回日本薬学会九州支部大会
    • Place of Presentation
      福岡
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.ph.sojo-u.ac.jp/~simoto/index.html

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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