Development of methodology for site-specific RNA modification using the Reactive Oligonucleotides
| Project/Area Number |
23790146
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Drug development chemistry
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| Research Institution | Sojo University |
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Principal Investigator |
IMOTO Shuhei 崇城大学, 薬学部, 講師 (20447189)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | RNA 修飾 / 架橋反応 / 核酸修飾 / 人工核酸 / RNA修飾 / クロスリンク |
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| Research Abstract |
RNA function is controlled by post-transcriptional modification. Therefore, there is increasing demand for methods of the site-specific modifications of RNA because of potential applications as biological tools and therapeutic methods. In this research, we are developing site-specific RNA modification tool using cross-linking forming oligonucleotide. We have found that the 6-vinylpurine newly designed in the current study serves well as a new nucleobase for increasing cross-link reactivity to target cytosine.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Production of truncated protein by the crosslink formation of mRNA with 2’-OMe oligoribonucleotide containing 2-amino-6-vinylpurine2012
Author(s)
Hagihara, S., Kusano, S., Lin, W.C., Chao, X.G., Hori, T., Imoto, S., Nagatsugi, F.
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Journal Title
Bioorg. Med. Chem. Lett.
Volume: 22
Issue: 12
Pages: 3870-3872
DOI
Related Report
Peer Reviewed
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