| Project/Area Number |
23790153
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental pharmacy
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| Research Institution | Oita University of Nursing and Health Sciences |
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Principal Investigator |
YOSHIDA Seiichi 大分県立看護科学大学, 看護学部, 准教授 (40360060)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 浮遊粒子状物質 / 次世代影響 / 免疫系 / 胎仔 / 遺伝子発現 / サイトカイン / 胎仔期曝露 / マウス / ケモカイン / マクロファージ / 好酸球 / Ahレセプター |
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| Research Abstract |
Prenatal exposure of concentrated airbone particulate matters (CAPs) has been shown to have an aggravate effect on immune system in male offspring. However, little is known about the mechanisms. In the present study we created a bronchial asthma mouse model by exposing offspring to ovalbumin (OVA), then we sequentially evaluated the effects of fetal exposure to two type of particles (CAPs and Asian sand dust) on worsening of allergic manifestations in OVA-exposed male offspring. We administered particle (200μg/animal) by intratracheal injection into dams during pregnancy on days 7 and 14 of gestation. Nine and twenty-four week-old male offspring were injected intratracheally with OVA (4 times at 2-week intervals). CAPs and Asian sand dust significantly increased the level of cytokine and chemokine levels in BALF. Our results suggest that fetal exposure to CAPs and Asian sand dust and postnatal intratracheal administration of OVA enhance allergic lung inflammation by activating the Th2-associated immune response through increase in cytokines and chemokines, which play an important role in bronchial asthma. Our findings suggest that the inhalation of CAPs and Asian sand dust by pregnant women has various effects on the fetus and may be involved in the increased prevalence of childhood allergies.
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