Characterization of bacterial iron transporter for the antioxidantability in Helicobacter pylori

• Project/Area Number: 23790156
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental pharmacy
• Research Institution: Keio University
• Principal Investigator: TSUGAWA Hitoshi 慶應義塾大学, 医学部, 特任助教 (30468483)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 酸化ストレス / 抗酸化能 / 慢性感染 / 薬剤耐性 / 鉄トランスポーター / ピロリ菌 / 鉄イオン / Superoxide Dismutase / Fur / 活性酸素 / 耐性菌

Research Abstract

Helicobacter pylori encodes a single iron-cofactored superoxide dismutase(SodB),which is regulated by the ferric uptake regulator (Fur). Ferrous ion (Fe^<2+>) is necessary for theactivation of SodB. The activity of SodB is an important determinant of the

Research Products

• [Journal Article] The tumor suppressormicroRNA-29c is downregulated andrestored by celecoxib in human gastriccancer cells. (2013)
  • Author(s): Saito Y., Suzuki H., Imaeda H., Matsuzaki J.,Hirata k., Tsugawa H., Hibino S., Kanai Y.,Saito H., Hibi T.
  • Journal Title: Int. J. Cancer Volume: 15 Pages: 1751-1760
  • Peer Reviewed
• [Journal Article] ピロリ菌のもつがん蛋白質CagAは酸化ストレスにより誘導されるオートファジーにより分解され (2012)
  • Author(s): 津川仁, 鈴木秀和
  • Journal Title: ライフサイエンス新着論文レビュー
  • URL: http://first.lifesciencedb.jp/
• [Journal Article] Reactiveoxygen species-induced autophagicdegradation of Helicobacter pylori CagA isspecifically suppressed in cancer stem-likecells (2012)
  • Author(s): Tsugawa H., Suzuki H, Saya H,Hatakeyama M, Hirayama T, Hirata K,Nagano O, Matsuzaki J, Hibi T
  • Journal Title: Cell Host Microbe Volume: 13 Pages: 764-777
  • Peer Reviewed
• [Journal Article] Overexpression of miR-142-5p and miR-155in gastric mucosa-associated lymphoid tissue(MALT) lymphoma resistant to Helicobacterpylori eradication (2012)
  • Author(s): Saito Y., Suzuki H., Tsugawa H., Imaeda H.,Matsuzaki J., Hirata K., Hosoe N.,Nakamura M., Mukai M., Saito H., Hibi T.
  • Journal Title: PLoS One Volume: 7
  • Peer Reviewed
• [Journal Article] Development of a novelmicroRNA promoter microarray forChiP-on-chip assay to identify epigeneticallyregulated microRNAs (2012)
  • Author(s): Saito Y., Suzuki H., Taya T., Nishizawa M.,Tsugawa H., Matsuzaki J., Hirata K., SaitoH., Hibi T
  • Journal Title: Biochem. Biophys.Res. Commun. Volume: 14 Pages: 33-37
  • Peer Reviewed
• [Journal Article] ミトコンドリア由来ROS依存性autophagyによる胃粘膜上皮のH.pylori由来がん蛋白質CagA排除システム (2012)
  • Author(s): 津川仁, 鈴木秀和,畠山昌則,平山壽哉,松崎潤太郎,平田賢郎,福原誠一郎,岡田佐和子,日比紀文
  • Journal Title: G.I.Reserch Volume: 20 Pages: 98-99
• [Journal Article] FecA1, a bacterial irontransporter, determines the survival ofHelicobacter pylori in the stomach. (2012)
  • Author(s): Tsugawa H., Suzuki H., Matsuzaki J.,Hirata K., Hibi T
  • Journal Title: FreeRadic. Biol. Med. Volume: 52 Pages: 1003-1010
  • Peer Reviewed
• [Journal Article] Efficacy ofsitafloxacin-based rescue therapy forHelicobacter pylori after failures of firstand second-line therapies (2012)
  • Author(s): Matsuzaki J., Suzuki H., Nishizawa T.,Hirata K., Tsugawa H., Saito Y., Okada S.,Fukuhara S., Hibi T
  • Journal Title: Antimicrob.Agents Chemother Volume: 56 Pages: 1643-1645
  • Peer Reviewed
• [Journal Article] Molecular approaches and modern clinicalstrategies for the management ofHelicobacter pylori infection in Japan. (2012)
  • Author(s): Suzuki H., Nishizawa T., Tsugawa H., HibiT
  • Journal Title: KeioJ. Med Volume: 61 Pages: 109-119
  • Peer Reviewed
• [Journal Article] Roles of oxidative stressin stomach disorders (2012)
  • Author(s): Suzuki, H., Nishizawa, T., Tsugawa, H.,Mogami S., Hibi, T
  • Journal Title: J. Clin. Biochem.Nutr Volume: 50 Pages: 35-39
  • Peer Reviewed
• [Journal Article] Helicobacter pylori感染細胞のoncoprotein CagA排除システム (2012)
  • Author(s): 津川仁, 鈴木秀和,畠山昌則,平山壽哉,松崎潤太郎,平田賢郎,福原誠一郎,岡田佐和子,日比紀文
  • Journal Title: 日本ヘリコバクター学会誌 Volume: 13(2) Pages: 40-43
• [Journal Article] Reactive Oxygen Species-Induced Autophagic Degradation of Helicobacter pylori CagA Is Specifically Suppressed in Cancer Stem-like Cells (2012)
  • Author(s): Tsugawa H, Suzuki H, Saya H, Hatakeyama M, Hirayama T, Hirata K, Nagano 0, Matsuzaki J, et al.
  • Journal Title: Cell Host & Microbe Volume: 12 Issue: 6 Pages: 764-777
  • DOI: 10.1016/j.chom.2012.10.014
  • Peer Reviewed
• [Journal Article] FecA1, a bacterial iron transporter, determines the survival of Helicobacter pylori in the stomach (2012)
  • Author(s): Tsugawa H, Suzuki H, Matsuzaki J, et al
  • Journal Title: Free Radic Biol Med Volume: 52 Issue: 6 Pages: 1003-1010
  • DOI: 10.1016/j.freeradbiomed.2011.12.011
  • Peer Reviewed
• [Journal Article] Molecular approaches and modern clinical strategies for the management of Helicobacter pylori infection in Japan (2012)
  • Author(s): Suzuki H., Nishizawa T., Tsugawa H., Hibi T.
  • Journal Title: Keio J. Med. Volume: 61 Pages: 109-119
  • NAID: 10031140763
  • Peer Reviewed
• [Journal Article] Efficacy of sitafloxacin-based rescue therapy for Helicobacter pylori after failures of first- and second-line therapies. (2012)
  • Author(s): Matsuzaki J., Suzuki H., Nishizawa T., Hirata K., Tsugawa H., Saito Y., Okada S., Fukuhara S., Hibi T.
  • Journal Title: Antimicrob. Agents Chemother. Volume: 56 Pages: 1643-1645
  • Peer Reviewed
• [Journal Article] Helicobacter pylori resistance to rifabutin inthe last 7 Years (2011)
  • Author(s): Nishizawa T., Suzuki H., Matsuzaki J.,Muraoka H., Tsugawa H., Hirata K. Hibi, T
  • Journal Title: Antimicrob. AgentsChemother Volume: 55 Pages: 5374-5375
  • Peer Reviewed
• [Journal Article] Enhancement ofamoxicillin resistance after unsuccessful Helicobacter pylori eradication (2011)
  • Author(s): Nishizawa T, Suzuki H, Tsugawa H,Muraoka H, Matsuzaki J, Hirata K, Ikeda F,Takahashi M, Hibi T.
  • Journal Title: Antimicrob.Agents Chemother. Volume: 55 Pages: 3012-3014
  • Peer Reviewed
• [Journal Article] 酸化ストレス研究により明らかになったHelicobacter pylori 薬剤耐性獲得機構 (2011)
  • Author(s): 津川仁, 鈴木秀和, 日比紀文
  • Journal Title: G.I.Reserch Volume: 19 Pages: 38-43
• [Journal Article] Roles of oxidative stress in stomach disorders (2011)
  • Author(s): Suzuki, H., et al.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 50 Issue: 1 Pages: 35-39
  • DOI: 10.3164/jcbn.11-115SR
  • NAID: 130004466668
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Enhanced bacterial efflux system is the first step to the development of metronidazole resistance in Helicobacter pylori. (2011)
  • Author(s): Tsugawa H., Suzuki H., Muraoka H., Ikeda F., Hirata K., Matsuzaki J., Saito Y., Hibi T.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 14 Issue: 2 Pages: 656-660
  • DOI: 10.1016/j.bbrc.2010.12.034
  • Peer Reviewed
• [Presentation] Specific accumulation ofCagA oncoprotein in CD44v9 expressingcancer stem cells (2013)
  • Author(s): 津川 仁、鈴木 秀和、佐谷 秀行、松崎潤太郎、平田 賢郎、福原 誠一郎、岡田佐和子、正岡 建洋、畠山 昌則、平山 壽哉、日比 紀文
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張
  • Year and Date: 2013-03-20
• [Presentation] Accumulation of Helicobacterpylori-derived CagA oncoprotein inCD44-variant expressing cancer stem-like cellsby escaping autophagy. (2013)
  • Author(s): Tsugawa H., Suzuki H., Saya H., HatakeyamaM., Hirayama T., Hirata K., Matsuzaki J., HibiT.
  • Organizer: The 6th InternationalGastrointestinal Consensus Symposium(IGICS)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2013-01-26
• [Presentation] H. pylori 感染細胞内ROS依存性autophagy発現によるCagA分解機序. (2012)
  • Author(s): 津川 仁, 鈴木 秀和, 畠山 昌則, 平山 壽哉, 松崎 潤太郎, 平田 賢郎, 日比 紀文
  • Organizer: 第85回日本生化学学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-15
• [Presentation] m1VacA依存性ROS 産生亢進によるoncoproteinCagA分解性autophagy発動機構 (2012)
  • Author(s): 津川 仁, 鈴木 秀和, 畠山 昌則, 平山 壽哉, 松崎 潤太郎, 平田 賢郎, 福原 誠一郎, 岡田 佐和子, 日比 紀文
  • Organizer: 第18回日本ヘリコバクター学会
  • Place of Presentation: 岡山
  • Year and Date: 2012-06-29
• [Presentation] H. pylori 感染宿主の細胞内ROS亢進依存的がん蛋白CagA排除メカニズム (2012)
  • Author(s): 津川 仁, 鈴木 秀和, 畠山 昌則, 平山 壽哉, 松崎 潤太郎, 平田 賢郎, 福原 誠一郎, 岡田 佐和子, 日比 紀文
  • Organizer: 第65回日本酸化ストレス学会
  • Place of Presentation: 徳島
  • Year and Date: 2012-06-07
• [Presentation] Specificamino acid mutation of p53 enhanced thestability of intracellular CagA by inhibitingautophagy induction (2012)
  • Author(s): Tsugawa, H., Suzuki, H., Hatakeyama, M., Hirayama, T., Matsuzaki, J., Hirata, K.,Fukuhara, S., Okada, S., Hibi, T.
  • Organizer: Digestive Disease Week2012, Research forum
  • Place of Presentation: San Diego, USA
  • Year and Date: 2012-05-21
• [Presentation] Degradation response of oncoproteinCagA by autophagy in host cell (2012)
  • Author(s): 津川 仁、鈴木 秀和、松崎 潤太郎、平田 賢郎、福原 誠一郎、岡田 佐和子、日比 紀文
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2012-03-28
• [Presentation] Degradation response of oncoprotein CagA by autophagy in host cell. (2012)
  • Author(s): 津川 仁、鈴木 秀和、松崎 潤太郎、平田 賢郎、福原 誠一郎、岡田 佐和子、日比 紀文.
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール （長崎県）
• [Presentation] Essential role of FecA1,iron-uptake transporter, in Helicobacterpylori for host colonization andmetronidazole resistance (2011)
  • Author(s): Tsugawa, H., Suzuki, H., Kanekawa, M.,Matsuzaki, J., Hirata, K., Okada, S., Fukuhara,S., Saito, Y., Hibi, T
  • Organizer: International Unionof Microbiological Societies (IUMS) 2011Congress
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2011-09-07
• [Presentation] Essentialrole of FecA1, iron-uptake transporter, inHelicobacter pylori for antioxidant abilityassociated with survival within human stomach. (2011)
  • Author(s): Tsugawa, H., Suzuki, H., Matsuzaki, J., Hirata,K., Okada, S., Fukuhara, S., Hibi, T.
  • Organizer: 5th Biennial Meeting of Society forFree Radical Research-Asia
  • Place of Presentation: Kagoshima,Japan
  • Year and Date: 2011-09-03
• [Presentation] 病原細菌の慢性感染解除論の新機軸構築に向けたH. pyloriのSodB発現維持システム解析 (2011)
  • Author(s): 津川 仁、鈴木 秀和、松崎 潤太郎、平田賢郎、福原 誠一郎、岡田 佐和子、日比 紀文
  • Organizer: 第64回日本酸化ストレス学会
  • Place of Presentation: 北海道ルスツリゾート
  • Year and Date: 2011-07-02
• [Presentation] Helicobacter pylori 感染細胞のoncoproteinCagA排除システム (2011)
  • Author(s): 津川 仁、鈴木 秀和、畠山 昌則、平山 壽哉、松崎 潤太郎、平田 賢郎、福原 誠一郎、岡田 佐和子、日比 紀文
  • Organizer: 第17回日本ヘリコバクター学会
  • Place of Presentation: 富山
  • Year and Date: 2011-06-25
• [Presentation] 酸化ストレス型宿主異物排除応答に抵抗するH.pyloriの鉄奪取機構 (2011)
  • Author(s): 津川 仁、鈴木 秀和、日比 紀文
  • Organizer: 第97回日本消化器病学会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-13
• [Presentation] The iron-uptake system for theantioxidant ability of Helicobacter pyloriregulated by Ferric uptake regulator (Fur). (2011)
  • Author(s): Tsugawa, H., Suzuki, H., Hirata, K.,Matsuzaki, J., Okada, S., Fukuhara, S., Saito,Y., Hibi, T
  • Organizer: Digestive Disease Week 2011
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-05-08
• [Presentation] pylori-derived oncoprotein,CagA is degraded by autophagy in human gastric epithelial cells (2011)
  • Author(s): Tsugawa, H., Suzuki, H., Hatakeyama, M.,Matsuzaki, J., Hirata, K., Okada, S., Fukuhara,S., Hibi, T. H.
  • Organizer: Digestive DiseaseWeek 2011, Research forum
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-05-07
• [Presentation] Essential role of FecA1, iron-uptake transporter, in Helicobacter pylori for host colonization and metronidazole resistance. (2011)
  • Author(s): Tsugawa, H., Suzuki, H., Kanekawa, M., Matsuzaki, J., Hirata, K., Okada, S., Fukuhara, S., Saito, Y., Hibi, T.
  • Organizer: International Union of Microbiological Societies (IUMS) 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター （北海道）
• [Presentation] Essential role of FecA1, iron-uptake transporter, in Helicobacter pylori for antioxidant ability associated with survival within human stomach. (2011)
  • Author(s): Tsugawa, H., Suzuki, H., Matsuzaki, J., Hirata, K., Okada, S., Fukuhara, S., Hibi, T.
  • Organizer: 5th Biennial Meeting of Society for Free Radical Research-Asia
  • Place of Presentation: Kagoshima Citizens' Culture Hall （鹿児島県）
• [Presentation] 病原細菌の慢性感染解除論の新機軸構築に向けたH. pyloriのSodB発現維持システム解析 (2011)
  • Author(s): 津川 仁、鈴木 秀和、松崎 潤太郎、平田 賢郎、福原 誠一郎、岡田 佐和子、日比 紀文.
  • Organizer: 第64回日本酸化ストレス学会
  • Place of Presentation: 北海道ルスツ （北海道）
• [Presentation] H. pylori-derived oncoprotein, CagA is degraded by autophagy in human gastric epithelial cells (2011)
  • Author(s): Tsugawa, H., Suzuki, H., Hatakeyama, M., Matsuzaki, J., Hirata, K., Okada, S., Fukuhara, S., Hibi, T.
  • Organizer: Digestive Disease Week 2011
  • Place of Presentation: Chicago, USA
• [Presentation] The iron-uptake system for the antioxidant ability of Helicobacter pylori regulated by Ferric uptake regulator (Fur). (2011)
  • Author(s): Tsugawa, H., Suzuki, H., Hirata, K., Matsuzaki, J., Okada, S., Fukuhara, S., Saito, Y., Hibi, T.
  • Organizer: Digestive Disease Week 2011
  • Place of Presentation: Chicago, USA
• [Presentation] H. pylori感染宿主の細胞内ROS亢進依存的がん蛋白CagA排除メカニズム
  • Author(s): 津川仁
  • Organizer: 第65回日本酸化ストレス学会
  • Place of Presentation: 徳島
• [Presentation] m1VacA依存性ROS産生亢進によるoncoprotein CagA分解性autophagy発動機構
  • Author(s): 津川仁
  • Organizer: 第18回日本ヘリコバクター学会
  • Place of Presentation: 岡山
• [Presentation] H. pylori 感染細胞内ROS依存性autophagy発現によるCagA分解機序
  • Author(s): 津川仁
  • Organizer: 第85回日本生化学学会
  • Place of Presentation: 福岡
• [Presentation] Specific amino acid mutation of p53 enhanced the stability of intracellular CagA by inhibiting autophagy induction
  • Author(s): Tsugawa Hitoshi
  • Organizer: Digestive Disease Week 2012
  • Place of Presentation: San Diego, USA
• [Presentation] Accumulation of Helicobacter pylori-derived CagA oncoprotein in CD44-variant expressing cancer stem-like cells by escaping autophagy
  • Author(s): Tsugawa Hitoshi
  • Organizer: The 6th International Gastrointestinal Consensus Symposium (IGICS)
  • Place of Presentation: Tokyo
• [Book] ピロリ菌の薬剤耐性機序とその対策. AnnualReview消化器 (2012)
  • Author(s): 鈴木 秀和, 津川 仁, 日比 紀文
• [Book] ヘリコバクター・ピロリ感染症の徹底検証. H.pylori の病原・薬剤耐性機序～慢性持続性感染による宿主応答から～ (2012)
  • Author(s): 鈴木 秀和, 津川 仁, 日比 紀文
  • Publisher: ライフ・サイエンス
• [Remarks]
  • URL: http://www.keio-med.jp/gastro/ugt/member/tugawa.html
• [Remarks] 研究代表者の所属研究室のwebページアドレス
  • URL: http://www.keio-med.jp/gastro/ugt/index.html