Molecular mechanism of CYP1A2 gene expression in male regulated bygrowth hormone for personalized medicine

• Project/Area Number: 23790177
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: University of Toyama
• Principal Investigator: KAWASAKI Yuki 富山大学, 大学院・医学薬学研究部(薬学), 助教 (30432107)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: CYP1A2 / 遺伝子発現制御 / 性差 / 遺伝子発現調節 / 転写因子

Research Abstract

To clarify the molecular mechanism regulating the CYP1A2 sex-specific expression, the effect of the growth hormone was examined. The continuous females specific growth hormone pattern treatment led the decreased CYP1A2 mRNA expression in the hepatocytes. The binding of Fra1 and C/EBPα to the Cyp1a2 gene 5’-flanking region was revealed.

Research Products

• [Journal Article] Udomsuk L., Jarukamjorn K., PutalunW., Sakuma T., Kawasaki Y., Nemoto N. (2011)
  • Author(s): Udomsuk L., Jarukamjorn K., PutalunW., Sakuma T., Kawasaki Y., Nemoto N.
  • Journal Title: J. Ethnopharmacol. Volume: 137 Pages: 902-908
  • Peer Reviewed
• [Journal Article] Modified expression of aryl hydrocarbon receptor-related genes by deoxymiroestrol, a phytoestrogen, in mouse hepatocytes in primary culture. (2011)
  • Author(s): Latiporn Udomsuk, Kanokwan Jarukamjorn, Waraporn Putalun, Tsutomu Sakuma, Yuki Kawasaki and Nobuo Nemoto.
  • Journal Title: Journal of Ethnopharmacology Volume: 137 Pages: 902-908
  • Peer Reviewed
• [Presentation] Constitutive androstane receptor リガンド誘導性発現調節を担うマウスCyp1a2 遺伝子の5'-上流域 (2013)
  • Author(s): 河崎優希, 古野幸美, 後藤雄真, 佐久間勉, 櫻井宏明,根本信雄
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
• [Presentation] Regulatory Elements for Transcriptional Activation of the mouse Cyp1a2 gene by Constitutive Androstane Receptor-Ligand (2012)
  • Author(s): Kawasaki Y., Furuno Y., Goto Y., Sakuma T., Sakurai H., Nemoto N.
  • Organizer: 50th Anniversary Symposium on Cytochrome P450 in Fukuoka
  • Place of Presentation: 福岡
• [Presentation] Constitutive androstane receptor アクチベーターによる誘導性発現調節を担うマウスCyp1a2遺伝子5'-上流域 (2012)
  • Author(s): 古野幸美, 河崎優希, 後藤雄真, 佐久間勉, 櫻井宏明, 根本信雄
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] Constitutive androstane receptor アクチベーターによる誘導性発現調節を担うマウスCyp1a2遺伝子5’-上流域 (2012)
  • Author(s): 古野幸美、河崎優希、後藤雄真、佐久間勉、櫻井宏明、根本信雄
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌市
• [Presentation] Constitutive androstane receptor responsive region in the 5'-flanking region of the mouse Cyp1a2 gene (2011)
  • Author(s): Kawasaki Y., Goto Y., Furuno Y., Sakuma T., Sakurai H., Nemoto N
  • Organizer: 第26回日本薬物動態学会年会
  • Place of Presentation: 広島
• [Presentation] PERIODIC FLUCTUATION OF CYP3A GENE EXPRESSION IN THE MOUSE LIVER (2011)
  • Author(s): Tsutomu Sakuma, Satomi Ikematsu, Yuka Kurimoto, Yuki Kawasaki and Nobuo Nemoto
  • Organizer: 第26回日本薬物動態学会年会
  • Place of Presentation: 広島市
• [Presentation] Constitutive androstane receptor responsive region in the 5’-flanking region of the mouse Cyp1a2 gene (2011)
  • Author(s): Yuki Kawasaki, Yuma Goto, Akira Honryo, Yukimi Furuno, Tsutomu Sakuma, Nobuo Nemoto
  • Organizer: 第26回日本薬物動態学会年会
  • Place of Presentation: 広島市
• [Presentation] Regulatory Elements for Transcriptional Activation of the mouse Cyp1a2 gene by Constitutive Androstane Receptor-Ligand
  • Author(s): Yuki Kawasaki, Yukimi Furuno, Yuma Goto, Tsutomu Sakuma, Hiroaki Sakurai and Nobuo Nemoto
  • Organizer: 50th Anniversary Symposium on Cytochrome P450 in Fukuoka
  • Place of Presentation: 九州大学（福岡市）
• [Presentation] Constitutive androstane receptor リガンド誘導性発現調節を担うマウスCyp1a2遺伝子の5’-上流域
  • Author(s): 河崎優希、古野幸美、後藤雄真、佐久間勉、櫻井宏明、根本信雄
  • Organizer: 日本薬学会第133回年会
  • Place of Presentation: パシフィコ横浜（横浜市）