Efficient generation of functional hepatocytes from human induced pluripotent stem cells and isolation of hepatic progenitor cells
| Project/Area Number |
23790193
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Tohoku Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2012 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 薬物動態 / 代謝学 / iPS 細胞 / 肝分化誘導法 / CYP / 肝特異的転写因子 / HNF / iPS細胞 / トランスポーター / 転写因子 |
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| Research Abstract |
The purpose of this study was to establish the generation of functional hepatocytes from human induced pluripotent stem cells (iPSCs). It revealed that the hepatic differentiation method using human HNF6-expressing adenovirus contributed to the efficient development of hepatocytes from iPSCs. HNF6 was introduced into the iPSHCs, CYP3A4 expression dramatically increased and the cells had the capability to metabolize testosterone. Our study proved for the first time that HNF6 transactivates the CYP3A4gene in hepatocyte-like cells differentiated from iPSCs.
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Report
(3 results)
Research Products
(34 results)
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[Journal Article] Association between Cancer Risk and Drug-metabolizing Enzyme Gene (CYP2A6, CYP2A13, CYP4B1, SULT1A1, GSTM1, and GSTT1) Polymorphisms in Cases of Lung Cancer in Japan2011
Author(s)
Tamaki, Y., Arai, T., Sugimura, H., Sasaki, T., Honda, M., Muroi, Y., Matsubara, Y., Kanno, S., Ishikawa, M., Hirasawa, N., Hiratsuka, M.
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Journal Title
Drug Metabolism and Pharmacokinetics
Volume: 26
Issue: 5
Pages: 516-522
DOI
NAID
ISSN
1347-4367, 1880-0920
Related Report
Peer Reviewed
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