Regulation of intra- or extracellular glucocorticoid concentration by Lipocalin2 as a candidate marker for steroid dosage.

Research Project

Project/Area Number	23790195
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Medical pharmacy
Research Institution	Josai International University
Principal Investigator	OZAWA Mika 城西国際大学, 薬学部, 助手 (40398558)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords	プロテオミクス / バイオマーカー / ステロイド / タンパク質 / 相互作用
Research Abstract	The aim of this study is to examine the hypothesis that lipocalin-2 binds to glucocorticoids, and regulates the concentration of intra- or extracellular free glucocorticoids. To examine the hypothesis, we established an assay for interaction between lipocalin -2 and glucocorticoids using surface plasmon resonance system. Our surface plasmon resonance analysis showed that lipocalin-2 interacts with dexamethasone dose-dependently , while the interaction of lipocalin-2 with prednisolone was not detected.. These results suggested that lipocalin-2 has a possibility of binding to specific glucocorticoids. We will examine the physiological role of lipocalin-2.

Report

(3 results)