Assessment of utility of saliva for lifestyle related disease screening
| Project/Area Number |
23790204
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Hoshi University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | 酸化ストレス / 糖尿病 / 唾液 |
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| Research Abstract |
An automated online solid-phase extraction method for the determination of the reduced and oxidized forms of thiols in mouse serum was developed and validated. Analysis was performed with column-switching hydrophilic interaction chromatography coupled with mass spectrometry (CS-HILIC-MS). The proposed CS-HILIC-MS method enabled the simultaneous determination of reduced and oxidized thiols in mouse serum samples. In addition, interference from endogenous compounds was removed by means of the column-switching technique. We also compared the effects of derivatization before and after preparing serum from blood samples and found that it was necessary to perform the derivatization immediately before preparing serum from blood samples. Serum reduced and oxidaized glutathione levels were significantly increased in diabetes mice. Our method is expected to be useful for the assessment of the roles of reduced and oxidized glutathione in the oxidative state.
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Report
(3 results)
Research Products
(8 results)
