Assessment of utility of saliva for lifestyle related disease screening

• Project/Area Number: 23790204
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Hoshi University
• Principal Investigator: IWASAKI Yusuke 星薬科大学, 薬学部, 助教 (10409360)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: 酸化ストレス / 糖尿病 / 唾液

Research Abstract

An automated online solid-phase extraction method for the determination of the reduced and oxidized forms of thiols in mouse serum was developed and validated. Analysis was performed with column-switching hydrophilic interaction chromatography coupled with mass spectrometry (CS-HILIC-MS). The proposed CS-HILIC-MS method enabled the simultaneous determination of reduced and oxidized thiols in mouse serum samples. In addition, interference from endogenous compounds was removed by means of the column-switching technique. We also compared the effects of derivatization before and after preparing serum from blood samples and found that it was necessary to perform the derivatization immediately before preparing serum from blood samples. Serum reduced and oxidaized glutathione levels were significantly increased in diabetes mice. Our method is expected to be useful for the assessment of the roles of reduced and oxidized glutathione in the oxidative state.

Research Products

• [Journal Article] Quantification of reduced and oxidized thiols in mouse serum by column-switching hydrophilic interaction chromatography coupled with mass spectrometry. (2011)
  • Author(s): Iwasaki Y, Nakano Y, Mochizuki K, OgawaT, Oda M, Ito R, Saito K, Nakazawa H
  • Journal Title: J. Pharm. Biomed. Anal. Volume: 56(1) Pages: 103-113
• [Journal Article] A new strategy for ionization enhancement by derivatization for mass spectrometry (2011)
  • Author(s): Iwasaki Y, Nakano Y, Mochizuki K, Nomoto M, Takahashi Y, Ito R, Saito K, Nakazawa H.
  • Journal Title: J. Chromatogr. B Volume: 879(17-18) Pages: 1159-1165
• [Journal Article] Quantification of reduced and oxidized thiols in mouse serum by column-switching hydrophilic interaction chromatography coupled with mass spectrometry. (2011)
  • Author(s): Iwasaki Y, Nakano Y, Mochizuki K, Ogawa T, Oda M, Ito R, Saito K, Nakazawa H.
  • Journal Title: J Pharm Biomed Anal. Volume: 56 Pages: 103-113
  • Peer Reviewed
• [Journal Article] A new strategy for ionization enhancement by derivatization for mass spectrometry. (2011)
  • Author(s): Iwasaki Y, Nakano Y, Mochizuki K, Nomoto M, Takahashi Y, Ito R, Saito K, Nakazawa H.
  • Journal Title: J Chromatogr B Analyt Technol Biomed Life Sci. Volume: 879 Pages: 1159-1165
  • Peer Reviewed
• [Presentation] (星薬大)質量分析法によるチオール化合物測定のためのマレイミド系誘導体化試薬の検討 (2012)
  • Author(s): 中野有紀、岩崎雄介、伊藤里恵、斉藤貢一、中澤裕之
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道
• [Presentation] 酸化および窒素化ストレスマーカー分析のための試料前処理方法の検討 (2012)
  • Author(s): 岩崎雄介
  • Organizer: 第23回クロマトグラフィー科学会議
  • Place of Presentation: 岐阜県
• [Presentation] (星薬大)酸化ストレス評価のための血清中チオール化合物の分析 (2011)
  • Author(s): 中野有紀、岩崎雄介、伊藤里恵、斉藤貢一、中澤裕之
  • Organizer: 第55回日本薬学会関東支部会
  • Place of Presentation: 千葉県
  • Year and Date: 2011-10-08
• [Presentation] (星薬大)親水性相互作用クロマトグラフィー/質量分 析法による唾液中グルタチオンの分析 (2010)
  • Author(s): 岩崎雄介、中野有紀、伊藤里恵、斉藤 貢 一、中澤裕之
  • Organizer: 日本分析化学会 第59年会