Search for metastasis-related proteins in colorectal cancer by proteome analysis

• Project/Area Number: 23790216
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: 独立行政法人医薬基盤研究所
• Principal Investigator: NAGANO Kazuya 独立行政法人医薬基盤研究所, 創薬基盤研究部, 研究員 (40548301)
• Co-Investigator(Renkei-kenkyūsha): TSUNODA Shin-ichi (独)医薬基盤研究所, バイオ創薬プロジェクト, プロジェクトリーダー (90357533) ; KAMADA Haruhiko (独)医薬基盤研究所, バイオ創薬プロジェクト, サブプロジェクトリーダー (00324509)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 医療薬剤学 / プロテオミクス / 癌 / 蛋白質 / 生体分子 / 薬学 / バイオマーカー / 転移

Research Abstract

Since metastasis is one of the most important prognostic factors in colorectal cancer, development of novel diagnostic and preventive methods for metastasis is highly desirable. However, the molecular mechanism of the metastatic phenotype has not been well elucidated. In this regard, we searched for metastasis-related proteins in colorectal cancer by analyzing the differential expression of proteins between primary and metastasis focus-derived colorectal tumor cells using a proteomics-based study. In order to validate these candidate proteins, we analyzed the expression profile of these proteins in tissue microarray. As a result, Rho GDP-dissociation inhibitor (Rho GDI) was identified as a metastasis-related protein in colon cancer patients. Consequently, Rho GDI may be useful for a diagnostic or preventive target against colon cancer metastasis.

Research Products

• [Journal Article] Rho GDP-dissociation inhibitor alpha is associated with cancer metastasis in colon and prostate cancer (2012)
  • Author(s): Yamashita T., Okamura T., Nagano K., Imai S., Abe Y., Nabeshi H., Yoshikawa T., Yoshioka Y., Kamada H., Tsutsumi Y., Tsunoda S
  • Journal Title: Pharmazie Volume: 67(3) Pages: 253-255
• [Journal Article] Rho GDP-dissociation inhibitor alpha is associated with cancer metastasis in colon and prostate cancer. (2012)
  • Author(s): Takuya Yamashita
  • Journal Title: Pharmazie Volume: 67 Pages: 253-255
  • Peer Reviewed
• [Presentation] Expression of Rho GDP dissociation inhibitor correlates positively with lymph node metastasis in colorectal cancer (2012)
  • Author(s): Nagano K., Okamura T., Yamashita T., Kanasaki S., Maeda Y., Inoue M., Abe Y., Kamada H., Tsutsumi Y., Tsunoda S
  • Organizer: EACR-22
  • Place of Presentation: Barcelona (Spain)
• [Presentation] 大腸がん転移診断・治療法の開発を目指した転移関連たんぱく質の探索 (2012)
  • Author(s): 長野一也, 岡村賢孝, 山下琢矢, 金崎聡一郎, 前田祐香, 古屋 剛, 井上雅己, 鍋師裕美, 吉川友章, 吉岡靖雄, 阿部康弘, 鎌田春彦, 堤 康央, 角田慎一
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌(北海道)
• [Presentation] Expression of Rho GDP dissociation inhibitor correlates positively with lymph node metastasis in colorectal cancer. (2012)
  • Author(s): Kazuya Nagano
  • Organizer: EACR-22
  • Place of Presentation: Barcelona (Spain)
• [Presentation] Proteomic profiling of proteins associated with metastasis in colorectal cancer cell lines (2011)
  • Author(s): Yamashita T., Nagano K., Yoshikawa T., Yoshioka Y., Itoh N., Abe Y., Kamada H., Tsutsumi Y., Tsunoda S
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋(愛知)
• [Presentation] プロテオミクスによる大腸がん転移関連たんぱく質の探索 (2011)
  • Author(s): 長野一也, 岡村賢孝, 山下琢矢, 堤 康央, 角田慎一
  • Organizer: 第20回日本がん転移学会学術集会・総会
  • Place of Presentation: 浜松(静岡)
• [Presentation] Proteomic profiling of proteins associated with metastasis in colorectal cancer cell lines. (2011)
  • Author(s): Yamashita T., Nagano K., Yoshikawa T., Yoshioka Y., Itoh N., Abe Y., Kamada H., Tsutsumi Y., Tsunoda S.
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋(愛知)