The molecular mechanism of membrane transport on the polarized epitherial cells.

• Project/Area Number: 23790226
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Osaka University
• Principal Investigator: YOSHIMURA Shinichiro 大阪大学, 医学系研究科, 助教 (60584521)
• Co-Investigator(Renkei-kenkyūsha): HARADA Akihiro 大阪大学, 大学院・医学系研究科, 教授 (40251441)
• Research Collaborator: NAKAJO Atsuhiro 大阪大学, 医学部医学科, 4回生
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: Rab8 / 上皮細胞 / Rab

Research Abstract

We identified a novel effector protein for Rab8, namely Rab8BP using yeast two-hybrid method. Rab8BP was co-localized with Rab8 in intracellular compartments. When Rab8BP was knocked-down by RNAi, the localization of Rab8 was altered. The results suggest that Rab8BP regulates the activity of Rab8 by helping its proper localization.

Research Products

• [Journal Article] Uncovering genes required for neuronal morphology by morphology-based gene trap screeningwith a revertible retrovirus vector. (2012)
  • Author(s): Y. Hashimoto, K. Muramatsu, M. Kunii, S. Yoshimura, M. Yamada, T. Sato, Y. Ishida, R. Harada, A. Harada.
  • Journal Title: FASEB J.
• [Journal Article] Rab14 and its exchange factor FAM116 link endocytic recycling and adherens junction stability inmigrating cells. (2012)
  • Author(s): A. Linford, S. Yoshimura, R. Nunes Bastos, L. Langemeyer, A. Gerondopoulos, D. J. Rigden, F.A. Barr.
  • Journal Title: Dev Cell. Volume: 5 Pages: 952-66
• [Journal Article] TBC1D14 regulates autopahgosomeformation via Rab11 and ULK1 positive recycling endosome (2012)
  • Author(s): A. Longatti, C.A. Lamb, M. Razi, S.Yoshimura, F.A. Barr, and S.A. Tooze.
  • Journal Title: J. Cell Biol. Volume: 197 Pages: 659-75
• [Journal Article] TBC1D14 regulates autophagosome formation via Rab11- and ULK1-positive recycling endosomes. (2012)
  • Author(s): Longatti A, Lamb CA, Razi M, Yoshimura S, Barr FA, Tooze SA.
  • Journal Title: Journal of Cell Biology Volume: 197 Issue: 5 Pages: 659-675
  • DOI: 10.1083/jcb.201111079
  • Peer Reviewed
• [Journal Article] Rab14 and its exchange factor FAM116 link endocytic recycling and adherens junction stability in migrating cells. (2012)
  • Author(s): Linford A, Yoshimura S, Nunes Bastos R, Langemeyer L, Gerondopoulos A, Rigden DJ, Barr FA.
  • Journal Title: Developmental Cell Volume: 22 Pages: 952-966
  • Peer Reviewed
• [Journal Article] Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector. (2012)
  • Author(s): Hashimoto Y, Muramatsu K, Kunii M, Yoshimura S, Yamada M, Sato T, Ishida Y, Harada R, Harada A.
  • Journal Title: FASEB Journal Volume: 26 Pages: 4662-4674
  • Peer Reviewed
• [Journal Article] The Role of VAMP7/TI-VAMP in Cell Polarity and Lysosomal Exocytosis invivo. (2011)
  • Author(s): M. Sato, S. Yoshimura, R. Hirai, A. Goto, M. Kunii, N. Atik, T. Sato, K. Sato, R. Harada, J. Shimada, T.Hatabu, H. Yorifuji, A. Harada.
  • Journal Title: Traffic Volume: 12 Pages: 1383-93
• [Journal Article] Analysis of Rab GTPase-Activating Proteins Indicatesthat Rab1a/b and Rab43 Are Important for Herpes Simplex Virus 1 Secondary Envelopment. (2011)
  • Author(s): H.L. Zenner, S. Yoshimura, F.A. Barr, C.M. Crump.
  • Journal Title: J Virol Volume: 85 Pages: 8012-21
• [Journal Article] Loss-of-function mutations in RAB18 cause Warburg micro syndrome. (2011)
  • Author(s): D.Bem , S. Yoshimura , R. Nunes-Bastos, F.C. Bond, M.A. Kurian, F. Rahman, M.T. Handley , Y.Hadzhiev, I.Masood, A.A Straatman-Iwanowska, A.R. Cullinane, A. McNeill, S .S. Pasha, G.A. Kirby, K.Foster, Z. Ahmed, J.E. Morton, D. Williams, J.M. Graham , W.B.
  • Journal Title: Am J Hum Genet Volume: 88 Pages: 499-507
• [Journal Article] Analysis of Rab GTPase-activating proteins indicates that Rab1a/b and Rab43 are important for herpes simplex virus 1 secondary envelopment. (2011)
  • Author(s): Zenner HL, Yoshimura S, Barr FA, Crump CM.
  • Journal Title: Journal of Virology Volume: 85 Pages: 8012-8021
  • Peer Reviewed
• [Journal Article] Loss-of-function mutations in RAB18 cause Warburg micro syndrome. (2011)
  • Author(s): Bem D, Yoshimura S, et. al.
  • Journal Title: The American Journal of Human Genetics Volume: 88 Pages: 499-507
  • Peer Reviewed
• [Journal Article] The role of VAMP7/TI-VAMP in cell polarity and lysosomal exocytosis in vivo. (2011)
  • Author(s): Sato M, Yoshimura S, Hirai R, Goto A, Kunii M, Atik N, Sato T, Sato K, Harada R, Shimada J, Hatabu T, Yorifuji H, Harada A.
  • Journal Title: Traffic Volume: 12 Pages: 1383-1393
  • Peer Reviewed
• [Presentation] 新規Rab8 結合タンパク質の機能解明 (2013)
  • Author(s): 吉村信一郎
  • Organizer: 第 118 回日本解剖学会総会・全国学術集会
  • Place of Presentation: 香川県高松市
• [Presentation] Rab とその調節因子によるメンブレントラフィックの制御 (2012)
  • Author(s): 吉村信一郎
  • Organizer: 第117回日本解剖学会総会・全国学術集会
• [Presentation] Rabとその調節因子によるメンブレントラフィックの制御 (2012)
  • Author(s): 吉村信一郎
  • Organizer: 第117回 日本日本解剖学会総会・全国学術集会(招待講演)
  • Place of Presentation: 山梨大学（甲府市）
• [Presentation] Rab タンパク質の一次繊毛形成への役割 (2011)
  • Author(s): 吉村信一郎
  • Organizer: 繊毛研究会 2011
  • Year and Date: 2011-06-29