| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Research Abstract |
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide involved in a variety of brain function. We demonstrated that PACAP regulates stathmin1 expression. Shumyatsky GP. et al. reported that mice without stathmin1 have deficiency in innate and learned fear. However, the detailed function and regulatory mechanism of stathmin1 in the central nervous system are unclear. In this study, we found that stathmin1 was phosphorylated during perinatal stage in the cortex. Phosphorylated stathmin1 was localized in the marginal zone of cortex, forming dot like fibers. In primary cultured neuron study, we detected dot-like phosphorylated stathmin1. And we indicated that BDNF was related to phosphorylation of stathmin1. PC12 cells over-expressed stathmin1 constitutive active form had longer thick neurites and numerous slight neurites as a filopodia. These results show that phosphorylated stathmin1 is related to filopodia or synapse constitution during perinatal period.
|
