| Project/Area Number |
23790233
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | Jichi Medical University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 下垂体前葉 / 3次元培養 / 組織構築 / 細胞外マトリックス / 濾胞星状細胞 / コラーゲン / ラミニン / ３次元培養 / 周皮細胞 / ゴナドトロフ |
|---|
| Research Abstract |
Aims of this project are (1) to establish 3 dimensional (3D) cell culture system using rat anterior pituitary cells and (2) to explore novel functions of anterior pituitary cells using the system. Applicant has applied a hanging drop 3D cell culture method to conventional primary cell culture of rat anterior pituitary cells and has developed a in vitro experimental model that is faithfully reproduced cell and extracellular matrix organization of in vivo anterior pituitary. Utilizing the culture system, applicant further investigated the function of folliculostellate cells that do not secrete classical anterior pituitary hormones and discovered that folliculostellate cells are essential in extracellular matrix formation, such as collagens and laminin, in the anterior pituitary gland.
|
