| Project/Area Number |
23790244
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Hokkaido University (2012)
Niigata University (2011) |
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Principal Investigator |
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| Research Collaborator |
HIBINO Hiroshi 新潟大学, 大学院・歯学総合研究科, 教授 (70314317)
YAMAGUCHI Kenichi 新潟大学, 大学院・医歯学総合研究科, 教授 (50108023)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 内耳 / 血管条 / 陽イオンチャネル / 重炭酸イオントランスポーター / NBCe1-B / 内耳蝸牛 |
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| Research Abstract |
The aim of this research is to clarify the system of pH regulation in stria vascularis in endocochlea. Using RT-PCR technique, transcripts of NBCe1-B, an electrogenic Na^+-HCO_3^- cotransporter, was shown to be expressed in stria vascularis. Results of immunostaining suggested that basal cells in stria vascularis expressed NBCe1-B. Application of DIDS, a NBCe1-B inhibitor, to perilymph reduced endocochlear potential measured from anesthetized guinea pigs. These results suggest that NBCe1-B plays an important role in pH regulation in stria vascularis.
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