| Project/Area Number |
23790247
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Mie University |
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Principal Investigator |
WANG Shujie 三重大学, 大学院・医学系研究科, 助教 (90567926)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 細胞運動 / 形態形成 / 細胞間相互作用 / 細胞極性 / 細胞遊走 / シグナル伝達 / Rhoファミリー |
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| Research Abstract |
Major purposes in this research are to reveal the regulatory mechanisms administrating a directional migration in motile cells. At the front of migrating cells, the higher activity of small GTPase Rac1 control the formation of focal adhesions and maintain adhesion sites toward substratum. Furthermore, Tiam1, the GDP/GTP exchange factor for Rac1, participates in polarized cell migration with the PAR complex of PAR3, PAR6, and atypical protein kinase C (aPKC) at adhesion sites. This research shows that Tiam1 interacts directly with talin, which binds and activates integrins to mediate their signaling, and also demonstrates that Tiam1 accumulates at adhesion sites in a dependent on talin and the PAR complex. Furthermore, this research also demonstrates the regulatory mechanisms controlling Rac1 activation and adhesion turnover for the polarized cell migration.
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