In vivo imaging of the dynamics of the regulation of bone metabolism

• Project/Area Number: 23790248
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Osaka University
• Principal Investigator: FUJIMORI Sayumi 大阪大学, 免疫学フロンティア研究センター, 特任研究員(常勤) (20589717)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 骨芽細胞 / 破骨細胞 / 骨リモデリング / 細胞間相互作用 / イメージング

Research Abstract

Little is known about the cellular behavior of osteoblasts and osteoclasts in bone tissue in vivo. To elucidate the mechanisms of the maintenance of bone homeostasis and the pathogenesis of bone diseases by visualization of the dynamics of bone remodeling in live bone tissues, we performed intravital two-photon imaging of osteoblasts and osteoclasts in the mouse calvarial bones in the physiological and pathological conditions such as osteoporosis.

Research Products

• [Journal Article] Dynamic live imaging of bone: opening a new era with 'bone histodynametry' (2013)
  • Author(s): Ishii, M., Fujimori, S., Kaneko, T., and Kikuta, J.
  • Journal Title: J Bone Miner Metab Volume: (in press)
• [Journal Article] Dynamic live imaging of bone: opening a new era with 'bone histodynametry'. (2013)
  • Author(s): Masaru Ishii
  • Journal Title: Journal of Bone and Mineral Metabolism Volume: in press Issue: 5 Pages: 507-511
  • DOI: 10.1007/s00774-013-0437-x
  • Peer Reviewed
• [Presentation] In vivoimaging of bone remodeling in bone tissue of the osteoblast-osteoclast dual labeled mice. (2013)
  • Author(s): Sayumi Fujimori, Masaru Ishii
  • Organizer: 2nd Joint Meeting of the International Bone and Mineral Society and the Japanese Society for Bone and Mineral Research
  • Place of Presentation: Kobe
  • Year and Date: 2013-05-29
• [Presentation] In vivo imaging of bone remodeling in bone tissue of the osteoblast-osteoclast dual labeled mice (2013)
  • Author(s): Sayumi Fujimori
  • Organizer: 2nd Joint Meeting of the International Bone and Mineral Society and the Japanese Society for Bone and Mineral Research
  • Place of Presentation: 神戸コンベンションセンター・神戸ポートピアホテル
• [Presentation] 生体マウス骨組織における骨リモデリングのin vivoイメージング (2012)
  • Author(s): 藤森 さゆ美, 石井 優
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] 生体イメージングによる骨代謝制御のダイナミクス解析 (2012)
  • Author(s): 藤森 さゆ美
  • Organizer: 第9回Skeletal Research Meeting
  • Place of Presentation: 京都
  • Year and Date: 2012-11-10
• [Presentation] 生体マウス骨組織における骨リモデリングのin vivoイメージング (2012)
  • Author(s): 藤森 さゆ美
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡