| Project/Area Number |
23790251
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Kagoshima University |
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Principal Investigator |
MINOBE Etsuko 鹿児島大学, 大学院・医歯学総合研究科, 助教 (00448581)
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| Research Collaborator |
MORI Masayuki 京都大学, 大学院・工学研究科, 准教授 (80342640)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | カルシウムチャネル / カルモジュリン / パッチクランプ法 / inside-out / ATP / run-down / リン酸化 / Cav1.2 / オカダ酸 / カルシウムイオン / 細胞内カルシウム |
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| Research Abstract |
Properties of the L-type Ca^channel and its mutants were explored using inside-out patch-clamp technique. Activity of these channels was induced by calmodulin plus ATP. A protein phosphatase inhibitor okadaic acid could substitute for ATP in wild-type channels, but it not in the mutant channels. It was suggested that the effects of ATP and okadaic acid, although apparently similar, were mediated by different mechanisms.
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